Aspects of the present disclosure include methods of producing a cellular suspension from a tissue sample by applying resonant acoustic energy to a container comprising the tissue sample in a manner sufficient to produce a cellular suspension from the tissue sample. Resonant acoustic mixers and kits for use in producing a cellular suspension from a tissue sample are also provided.

A liquid mixing system includes a support housing at least partially bounding a compartment. A mount is secured to the support housing. A drive motor assembly is configured to engage a drive shaft for moving the drive shaft within the compartment of the support housing. A four bar linkage system extends between the mount and the drive motor assembly, the four bar linkage system being movable between a first position wherein the drive motor assembly is disposed at a first elevation and a second position wherein the drive motor assembly is disposed at a second elevation that is different from the first elevation.

An aggregated cell mass dispersing device (100) of the present invention includes: a storage container (101) storing a liquid (L) containing an aggregated cell mass; a pipette (102) whose tip is pushed against an inner bottom surface (101a) of the storage container; and an elastic body (103) supporting an outer bottom surface of the storage container.

The present set of embodiments relate to a system, method, and apparatus for culturing cells within a cell culture vessel having a mixing element. The cell culture system includes a flexible portion and a mixing element disposed therein. The mixing element includes a suspended foldable portion. The system is configured to reduce shear stress on cells without compromising mixing efficiency. This reduction is accomplished by using a mixing element having a large surface area allowing for reduced rotational speeds. The system is collapsible for ease of transport and disposal. The flexible portion collapses and the foldable portion folds to minimize the volume of the system while not in operation.

A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface. The bag has a bottom end wall that includes a first sparger and a second sparger. The first sparger and the second sparger each have a flexible first sheet overlying a flexible second sheet, the first sheet and the second sheet being secured together so as to form a sparging area bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the sparging area being gas permeable so that gas can pass from the sparging area, through the first sheet, and into the chamber.

The insert shelf (1) is characterized in that it has at least one means (3) with which an incubation medium located in an incubation vessel placed on the insert shelf (1) can be set in motion during an incubation treatment or during an incubation process in an incubator (2). For this purpose, the at least one means (3) can be operated electrically and/or preferably moved mechanically.

Method for operating an agitating device and a digester wherein the digester (1) is filled with a substrate (7) and wherein an agitating device (10) controlled by a control device (50) is disposed in the digester (1). The following process steps are carried out. a) A target load curve (60) is lodged in the control device (50) b) the control device (50) prescribes a target speed of rotation (61) c) the control device (50) operates the agitating device (10) at an actual speed of rotation (71) corresponding to the prescribed target speed of rotation (61); d) the control device captures an actual measurement value (81) which is characteristic of the torque of the agitating device (10) at the actual speed of rotation (71); e) the control device (50) derives from the actual measurement value (81) an actual characteristic value (91) of the applied torque of the agitating device (10); f) the control device (50) compares the derived actual characteristic value (73) against the target characteristic value (63) of the substrate (7) resulting from the target load curve (60) at the prescribed target speed of rotation (61); g) the control device (50) controls the agitating device (10) in dependence on the result of comparison.

The present invention relates to a biological material extraction apparatus. According to the present invention, a tube in which a solution for use in extracting a biological material is contained is rotated to directly mix the solution, and cells where stirring is being conducted in each step are sealed to fundamentally block stirring-induced pollution between cells, thereby improving an extraction efficiency.

A blending tube (10), comprising: a tube body (20), the tube body (20) being used for accommodating a sample; and a first stirring member (30) and a second stirring member (40), which are arranged on an inner wall (200) of the tube body (20), wherein a width-to-thickness ratio of the first stirring member (30) is greater than a width-to-thickness ratio of the second stirring member (40).

A liquid mixing system includes a support housing at least partially bounding a compartment. A mount is secured to the support housing. A drive motor assembly is configured to engage a drive shaft for moving the drive shaft within the compartment of the support housing. A four bar linkage system extends between the mount and the drive motor assembly, the four bar linkage system being movable between a first position wherein the drive motor assembly is disposed at a first elevation and a second position wherein the drive motor assembly is disposed at a second elevation that is different from the first elevation.