An amphoteric dissociation ion exchange separation medium, the surface of which is an amphoteric dissociationcovalently-modified layer. When an environmental pH value is lower than the isoelectric point, pIm, of the covalently-modified layer, the type of net charges on the surface of the covalently-modified layer is positive and the separation medium has the properties of an anion exchanger; when the environmental pH value is higher than the pIm, the type of net charges on the covalently-modified layer surface is negative and the separation medium has the properties of acation exchanger. The separation medium has the properties of an anion exchanger and a cation exchanger at both sides of the pIm, respectively. The pH of an eluent can be adjusted to allow the separation medium surface and the target substance to have the same type of net charges, so that the target substance can be released by electrostatic repulsion.

The present invention provides a method for the production of whey proteins in a single step process using combination of chromatography and membrane filtration technique, comprising treating cotton cloth with a mixture of chlorosulphonic acid and chloroform and then subsequently treating it with chloroform, dilute NaOH, glycine and water to recover modified cotton cloth as the product, thereafter fixing product in a membrane filtration device equipped with modified flow pattern and then equilibrating it with equilibration buffer, followed by loading of whey for adsorption of protein on the product and washing of the product with equilibration buffer, thereafter elution of adsorbed proteins with elution buffer, and then regeneration of the product by treating it with dilute HCl and water to reuse the product.

The present invention provides a method for the production of whey proteins in a single step process using combination of chromatography and membrane filtration technique, comprising treating cotton cloth with a mixture of chlorosulphonic acid and chloroform and then subsequently treating it with chloroform, dilute NaOH, glycine and water to recover modified cotton cloth as the product, thereafter fixing product in a membrane filtration device equipped with modified flow pattern and then equilibrating it with equilibration buffer, followed by loading of whey for adsorption of protein on the product and washing of the product with equilibration buffer, thereafter elution of adsorbed proteins with elution buffer, and then regeneration of the product by treating it with dilute HCl and water to reuse the product.

The present invention describes a method for isolating extracellular vesicles (EVs) from different biological fluids, said nickel-based isolation method (NBI) is fast, scalable and allows for the purification of dimensionally heterogeneous EVs at physiological pH, preserving their integrity and stability in solution.

The present invention describes a method for isolating extracellular vesicles (EVs) from different biological fluids, said nickel-based isolation method (NBI) is fast, scalable and allows for the purification of dimensionally heterogeneous EVs at physiological pH, preserving their integrity and stability in solution.

Disclosed is an anion exchange porous medium, e.g., a porous membrane, that includes a porous support and a crosslinked cationic polymer coating disposed thereon, wherein the cationic polymer of the crosslinked cationic polymer coating comprises polymerized monomer (A) and polymerized monomer (B) wherein A and B are as defined herein. Also disclosed are methods of preparing the anion exchange porous medium and of treating a fluid containing a biologic.

Disclosed is an anion exchange porous medium, e.g., a porous membrane, that includes a porous support and a crosslinked cationic polymer coating disposed thereon, wherein the cationic polymer of the crosslinked cationic polymer coating comprises polymerized monomer (A) and polymerized monomer (B) wherein A and B are as defined herein. Also disclosed are methods of preparing the anion exchange porous medium and of treating a fluid containing a biologic.

Hybrid sorbent on the base of anion-exchange polymeric matrix with HFO for selective sorption of arsenic characterized in that, HFO exists in matrix as particles, which at most are amorphous ferrihydrite, fraction of which is not less than 80%, preferably more than 90% from total mass of HFO.

The object of the invention and the technical result achieved with the use of the invention is to develop new hybrid sorbent with HFO with increased sorption kinetics of two arsenic forms As(III) and As(V) simultaneously.

Hybrid sorbent on the base of anion-exchange polymeric matrix with HFO for selective sorption of arsenic characterized in that, HFO exists in matrix as particles, which at most are amorphous ferrihydrite, fraction of which is not less than 80%, preferably more than 90% from total mass of HFO.

The object of the invention and the technical result achieved with the use of the invention is to develop new hybrid sorbent with HFO with increased sorption kinetics of two arsenic forms As(III) and As(V) simultaneously.

An adsorbent for phosphoric acid-based anionic substances, that contains foamed glass, and that has a Ca2p concentration of 7.5 atom % or more or a Na1s concentration of 5.0 atom % or less at the surface thereof as measured by XPS analysis, and a half-width of Si2p peak of 2.4 eV or more. The adsorbent can also have a specific surface area of 45 m.sup.2/g or more or a pore volume of 2.5 cm.sup.3/g or more as measured by mercury intrusion.