Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.

The invention provides a method of thawing a sample comprised in a container, the method comprising the steps of: a) calculating an agitation program as a function of either or both of the sample volume and the type of the container, and the ice fraction in the sample, and optionally the thermal conductivity of the sample container; b) agitating said sample according to the program to agitate at least one region of the sample; and c) changing the agitation program applied to at least one region of the sample in response to changes in the sample volume and/or sample ice fraction. The invention further provides a method of reducing shearing to cells during a method of agitation, and methods for thawing a sample wherein a sample container is differentially heated. An apparatus for use in the methods is also provided, as is an apparatus for thawing and/or cooling a sample which comprises a resilient vessel wall.

Variable temperature analytical assemblies are provided and/or methods for changing temperatures of a mass are provided. Low thermal conductance components and/or assemblies are also provided. Methods for thermally isolating a mass from a support structure are also provided.

The invention provides an alternating current electrospray technology that can generate micron sized droplets in oil at very high throughput for emulsion or digital PCR (Polymerase Chain Reaction). This technology outperforms the throughput of the current gold standard in droplet generation using flow-focusing technology by at least a factor of 100. The design is simple and can generate a billion to a trillion monodispersed droplets in about one hour. This is much faster than flow-focusing which is limited to a few million droplets per hour. The droplet size and generation rate can also be easily adjusted by changing the voltage of the AC electric field. The range of produced droplet sizes is about 1-100 microns, wherein the droplets are monodispersed in size.

Systems and methods for light based heating of light absorbing sources for modification of nucleic acids through fast thermal cycling of polymerase chain reaction are described.

A laboratory device includes an outer surface section. The laboratory device further includes a display apparatus that displays a respective temperature state of the outer surface section. In addition, the laboratory device utilizes at least also heat of the outer surface section for the energy supply of the display apparatus.

A test sensor heating system is disclosed that provides the desired and different temperatures to at least two different reaction zones based on a fixed potential. The measurement device does not alter the potential applied to the heating system in response to temperature feedback information. The heating system provides the desired and different temperatures to the different reaction zones of the test sensor by varying heating element spacing and/or the resistivity of an associated resistive layer of the test sensor to provide the desired temperature in response to the fixed potential. The system also may provide two or more different temperature zones to the test sensor by using different heating element spacing and/or resistive layer resistivity at different locations of the test sensor.

A reaction processor includes: a vessel installation unit for installing a reaction processing vessel provided with a channel formed in a substrate; a high temperature heater and a medium temperature heater for adjusting the temperature of the channel of the reaction processing vessel; a vessel alignment mechanism for adjusting the position of the reaction processing vessel 10; and a housing that has a housing main unit and a cover portion capable of being opened and closed with respect to the housing main unit and that houses the vessel installation unit, the high temperature heater, the medium temperature heater, and the vessel alignment mechanism. In conjunction with the state of the cover portion being changed from an open state to a closed state, the vessel alignment mechanism aligns the reaction processing vessel such that the reaction processing vessel can be heated by the high temperature heater and the medium temperature heater.

A test sensor heating system is disclosed that provides the desired and different temperatures to at least two different reaction zones based on a fixed potential. The measurement device does not alter the potential applied to the heating system in response to temperature feedback information. The heating system provides the desired and different temperatures to the different reaction zones of the test sensor by varying heating element spacing and/or the resistivity of an associated resistive layer of the test sensor to provide the desired temperature in response to the fixed potential. The system also may provide two or more different temperature zones to the test sensor by using different heating element spacing and/or resistive layer resistivity at different locations of the test sensor.

A fluid testing device may include a fluid interaction element and a fluid chamber to contain a fluid to be sensed by the fluid interaction element. The fluid chamber may form a first gap through which fluid is to be wicked to a second gap that is opposite the fluid interaction element and less than the first gap.