The present invention relates to a microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, the present invention relates to an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, the present invention relates to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets. The microfluidic device comprises a transfer conduit comprising a first transfer conduit part having a first affinity for water; and a collection conduit comprising a first collection conduit part having a second affinity for water being different from the first affinity for water. A well section and a microfluidic section of the microfluidic device are fixedly connected to each other.

A flow stabilized chip includes a chip mainbody, a buffering chamber and two fluid delivery ports. The chip mainbody has a pipe-connection surface. The buffering chamber is disposed in the chip mainbody. The two fluid delivery ports are disposed on the pipe connection surface and connected to the buffering chamber. The chip mainbody includes, in order from the pipe-connection surface to a bottom of the chip mainbody, a first base plate, a first elastic membrane, a second base plate, a second elastic membrane and a third base plate. The first base plate includes a first opening. The second base plate includes a second opening. The third base plate includes a third opening. The first elastic membrane, the second base plate and the second elastic membrane are stacked in sequence to form the buffering chamber.

Embodiments herein relate to non-invasive bladder cancer detection systems and methods. In an embodiment, a method for detecting a disease state in a subject is included. The method includes obtaining a liquid biological sample from the subject and placing it into a container and contacting the liquid biological sample with a first chemical sensor element, where the first chemical sensor element can include a plurality of discrete graphene varactors. The method can include sensing and storing capacitance of each of the discrete graphene varactors to obtain a first sample data set. Other embodiments are also included herein.

In an embodiment, a device includes: an electrode configured to change a contact angle of a liquid droplet above the electrode when a first voltage is applied to the electrode; a sensing film overlaying the electrode, wherein the electrode is configured for assessment of a state of the liquid droplet based on a second voltage sensed at the electrode; a reference electrode above the electrode, the reference electrode configured to provide a reference voltage; and a microfluidic channel between the electrode and the reference electrode, wherein the microfluidic channel is configured to manipulate the liquid droplet using the electrode.

A microfluidic chip and a driving method thereof are provided. The microfluidic chip includes a base substrate, a driving circuit array, a first decoding circuit, and a second decoding circuit, the driving circuit array, the first decoding circuit, and the second decoding circuit are all integrated on the base substrate; the first decoding circuit is configured to generate and output a target scan driving signal to the driving circuit array; the second decoding circuit is configured to generate and output a target driving voltage signal to the driving circuit array; and the driving circuit array is configured to control an operation of a liquid droplet over the driving circuit array based on the target scan driving signal and the target driving voltage signal.

Provided are methods of engulfing particles into droplets. The methods use a microfluidic device comprising a droplet generator and a chamber, the chamber comprising a liquid medium disposed on a voltage supply electrode and a ground electrode. The methods comprise dispensing a particle and a droplet into the liquid medium, and dielectrophoretically trapping the particle and the droplet using the voltage supply electrode and the ground electrode. The methods further comprise engulfing the particle into the droplet, wherein the engulfing comprises increasing a supply voltage between the voltage supply electrode and the ground electrode, thereby moving the droplet toward the voltage supply electrode and engulfing the particle into the droplet. In certain embodiments, the methods further comprise modifying the engulfed particle, assessing the engulfed particle, or both. Devices that find use in practicing the methods of the present disclosure are also provided.

In an embodiment, a device includes: an electrode configured to change a contact angle of a liquid droplet above the electrode when a first voltage is applied to the electrode; a sensing film overlaying the electrode, wherein the electrode is configured for assessment of a state of the liquid droplet based on a second voltage sensed at the electrode; a reference electrode above the electrode, the reference electrode configured to provide a reference voltage; and a microfluidic channel between the electrode and the reference electrode, wherein the microfluidic channel is configured to manipulate the liquid droplet using the electrode.

The present invention relates to a test system or an assay system (detection system) and test method preferably for use in the Point-of-Care (PoC) field.

Examples include microfluidic devices. Example microfluidic devices include a first microfluidic channel, a second microfluidic channel, and a third microfluidic channel fluidly coupled to the first microfluidic channel and the second microfluidic channel via a fluid junction. A fluid actuator is disposed in the third microfluidic channel to actuate to thereby pump a first fluid and a second fluid into the third microfluidic channel.

In an embodiment, a device includes: an electrode configured to change a contact angle of a liquid droplet above the electrode when a first voltage is applied to the electrode; a sensing film overlaying the electrode, wherein the electrode is configured for assessment of a state of the liquid droplet based on a second voltage sensed at the electrode; a reference electrode above the electrode, the reference electrode configured to provide a reference voltage; and a microfluidic channel between the electrode and the reference electrode, wherein the microfluidic channel is configured to manipulate the liquid droplet using the electrode.