An immunoassay device for use in quantitatively measuring an amount of an analyte in a fluid sample, employs reagents that include particle pairs comprising a) one of an antigen and antibody coupled with a label, and b) a magnetic particle coupled with the other of the antigen and antibody. A transport which moves a set of reaction wells along a path and a dispenser dispenses respective ones of the reagents into the reaction wells. Prior to magnetic separation and optical analysis, a controller that coordinates movement of the transport with operation of the pipette modules operates the transport to reciprocate the set of reaction wells along the path for mixing the fluid sample with the reagents.

A digital microfluidic chip and a digital microfluidic system. The digital microfluidic chip comprises: an upper substrate and a lower substrate arranged opposite to each other; multiple driving circuits and multiple addressing circuits disposed between the lower substrate and the upper substrate; and a control circuit, electrically connected to the driving circuits and the addressing circuits. The control circuit is configured to apply, in a driving stage, a driving voltage to each driving circuit, such that a droplet is controlled to move inside a droplet accommodation space according to a set path, measure, in a detection stage, after a bias voltage is applied to each addressing circuit, a charge loss amount of each addressing circuit, and to determine the position of the droplet according to the charge loss amount. The charge loss amount of each addressing circuit is related to the intensity of received external light.

The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.

Systems, methods, and apparatuses are provided for self-contained nucleic acid preparation, amplification, and analysis.

Micro-Organosphers, including Patient-Derived Micro-Organospheres (PMOSs), apparatuses and methods of making them, and apparatuses and methods of using them. Also described herein are methods and systems for screening a patient using these Patient-Derived Micro-Organospheres, including personalized therapies.

A sensing device includes a sample loading chamber configured to receive a sample, a detection antibody drying or lyophilization chamber configured to receive a first portion of the sample, one or more substrate drying or lyophilization chambers configured to receive a second portion of the sample, and one or more reaction chambers connected to the detection antibody drying or lyophilization chamber and the one or more substrate drying or lyophilization chambers. The detection antibody drying or lyophilization chamber and one or more substrate drying or lyophilization chambers are placed in parallel between the sample loading chamber and the one or more reaction chambers.

Provided is an amplification module with a gas moving passage and an extract moving passage, more particularly an amplification module in which, when an extract is input from a genome extraction device in which the amplification module is installed, a quantitative amount of the extract is input to each accommodating portion so that the accuracy of detection can be increased.

A microfluidic device includes a bottom electrode, a dielectric layer on the bottom electrode, one or more top electrodes on a region of the dielectric layer, Each of the one or more top electrodes has a sidewall that forms a sidewall angle with an outer surface of the dielectric layer that is less than 180 degrees. The sidewall of each of the one or more top electrodes and a portion of the outer surface of the dielectric layer adjacent to the sidewall define a microchannel region for transporting an open microchannel of a fluid. Such microfluidic devices may enable transport of small microchannels using low voltages.

Fluidic devices, systems, and methods for analyzing an analyte are described. In an embodiment, the fluidic devices include a housing defining a lysis chamber shaped to receive a biological sample; a lysis buffer storage chamber disposed within the housing and carrying a lysis buffer configured to lyse cells of the biological sample; a cap configured to cooperatively couple to the housing; a compressor configured to compress the lysis buffer storage chamber and expel the lysis buffer from the lysis buffer storage chamber and into the lysis chamber when the cap is uncoupled from the housing; and a porous membrane in selective fluidic communication with the lysis chamber.

A method of conducting a biological assay, comprises obtaining data corelative to a temperature of a reagent, mixing the reagent with a sample to provide a mixture, receiving from the mixture a signal indicative of an amount of an analyte in the sample, and correcting the amount based on the obtained data and on a type of the reagent.