Systems and methods for flow cells are provided. Flow cells may encompass a range of fluidic devices for various applications ranging from microfluidic systems to bulk phase flow systems. Flow cells may comprise one or more components for passive or active fluid transfer. Descriptions are provided for advantageous methods of fabricating flow cells for particular applications such as biological assays. Provided is a composition, comprising a first substrate comprising a first covalently-bound ligand; and a second substrate comprising a second covalently-bound ligand; wherein the first covalently-bound ligand and the second covalently-bound ligand are covalently bonded to form a heterocyclic compound. Also provided is a flow cell device, comprising: a first substrate comprising a microfabricated surface; and a second substrate comprising a non-patterned surface; wherein the first substrate is joined to the second substrate to form an enclosure; and wherein the microfabricated surface comprises at least one chamber, wherein the chamber comprises a microarray of active sites with specific functionalization separated by an optically resolvable distance and a functionalized surface comprising a passivating group or a blocking group; and wherein each active site of the microarray of active sites comprises a capture agent.

The present invention provides a magnetic sorting microfluidic chip, including a substrate, a chip model material layer, a micro-channel unit and a magnetic sorting unit, where the chip model material layer is disposed on the substrate, and the micro-channel unit and the magnetic sorting unit are both disposed in the chip model material layer; the micro-channel unit includes a sorting channel and magnetic pole channels; the sorting channel is provided with a plurality of sorting channel inlets and a plurality of sorting channel outlets; and the magnetic sorting unit includes permanent magnets, high-permeability alloys, and magnetic pole arrays disposed in the magnetic pole channels, where the high-permeability alloys are configured to conduct magnetic fields of the permanent magnets to the magnetic pole arrays, so that the magnetic pole arrays generate magnetic fields having opposite polarities on left and right positions of the sorting channel.

In one example, a method of preparing a fluidic channel includes covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover. The covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel.

In an example of a method for making a flow cell, a metal material is sputtered over a transparent substrate including depressions separated by interstitial regions to form a metal film having a first thickness over the interstitial regions and having a second thickness over the depressions, the second thickness being about 30 nm or less and being at least ⅓ times smaller than the first thickness. A light sensitive material is deposited over the metal film; and the metal film is used to develop the light sensitive material through the transparent substrate to define an altered light sensitive material at a first predetermined region over the transparent substrate. The altered light sensitive material is utilized to generate a functionalized layer at the first predetermined region or at a second predetermined region over the transparent substrate.

A heat sealing product suitable for seating one or more individual containers, said heat sealing product comprising: (i) a plurality of individual heat seals set out in a configuration substantially corresponding to the shape and configuration of the container(s) to be sealed, the size and shape of the individual heat seals corresponding substantially to the size and shape of the tops of the individual container(s) to be sealed; (ii) a peelable support film layer coated on one side with a low tack adhesive, the low tack adhesive serving to hold the individual heat seals in place on the support film layer in the desired configuration prior to the sealing process; (iii) alignment points in the sealing product adapted to enable the heat sealing product and therefore the individual heat seals of the heat sealing product to be aligned substantially exactly with respect to the individual containers to be sealed.

Double bag having a first compartment containing a composition to be distributed and a second compartment for receiving a used fluid, a first bag connector communicating with the first compartment and serving to empty the latter, and a second bag connector communicating with the second compartment and serving to fill the latter.

A liquid discharging device to be used with a liquid dispensing apparatus includes a discharging portion configured to discharge a liquid based on a control signal from the liquid dispensing apparatus on which the liquid discharging device is mounted, and a sheet material having a characteristic configured to be changed by the liquid dispensing apparatus after a discharge of the liquid by the discharging portion.

An apparatus includes a fluid reservoir and a laminate fluidic circuit positioned above the fluid reservoir. The laminate fluidic circuit includes two or more layers laminated together to define a substantially planar substrate and one or more channels defined within the substrate. The laminate fluidic circuit includes a flexible conduit defined by a portion of the substrate encompassing an extent of at least one of the channels that is partially separated or separable from the remainder of the substrate. The flexible conduit is deflectable with respect to the planar substrate toward the fluid reservoir such that the flexible conduit fluidly connects the at least one channel to the fluid reservoir.

A microfluidic arrangement for manipulating fluids is provided. The microfluidic arrangement comprises a substrate, a first fluid and a second fluid, which is immiscible with the first fluid. The first fluid is arranged to be at least partially covered by the second fluid. The first fluid is arranged in a desired shape on an unpatterned surface of the substrate. The first fluid is retained in said shape by a fluid interface between the first and second fluids. A microfluidic arrangement comprising an array of drops is also provided. The microfluidic arrangement comprises a substrate, a first fluid and a second fluid, which is immiscible with the first fluid. The first fluid is arranged to be at least partially covered by the second fluid. The first fluid is arranged to be covered at least partially by the second fluid. The first fluid is arranged in a given array of drops on an unpatterned surface of the substrate. Each drop cross section area having a (height:width) aspect ratio of (1:2) or less. A method of fabricating a microfluidic arrangement for manipulating fluids is also provided. The method comprises arranging a first fluid on an unpatterned surface of a substrate in a desired shape. The method also comprises arranging a second fluid, which is immiscible with the first fluid, to cover the first fluid at least partially. The first fluid is retained in said shape by a fluid interface between the first and second fluids. The method also comprises drying the first fluid to form a residue in said shape on the substrate.

A fluidic device for processing a fluid or species therein is described. The device comprises a 3D channel including an inlet for receiving a sample fluid and an outlet for outputting the sample fluid. The channel is adapted for guiding flow of the sample fluid in an axial direction from the inlet to the outlet. The channel includes at least two side walls. The device also has a controllable flow inducer having electrodes for inducing, when the sample fluid is flowing through the channel, a motion of the sample fluid in the channel in a plane substantially orthogonal to the axial direction. Along at least one of the side walls at least part of the electrodes are formed by alternatingly at least an electrically conducting portion, an electrically insulating portion and a further electrically conducting portion.