An exemplary mobile impedance-based flow cytometer is developed for the diagnosis of sickle cell disease. The mobile cytometer may be controlled by a computer (e.g., smartphone) application. Calibration of the portable device may be performed using a component of known impedance value. With the developed portable flow cytometer, analysis may be performed on two sickle cell samples and a healthy cell sample. The acquired results may subsequently be analyzed to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals may be observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.

A molecular diagnostics apparatus is provided. The molecular diagnostics apparatus is adapted to perform DNA chain replication to one sample. The molecular diagnostics apparatus includes a bracket, a central control module, a motor, a magnetic unit, a rotational carrier, a detection module and at least one power supply coil. The central control module is disposed on the bracket. The motor is disposed on the bracket, wherein the central control module drives the motor. The magnetic unit is disposed on the bracket, wherein the magnetic unit provides a magnetic field. The motor is adapted to rotate the rotational carrier. The rotational carrier is rotated relative to the bracket. The sample is disposed on the rotational carrier. The detection module is disposed on the rotational carrier. The power supply coil is coupled to the detection module, and disposed on the rotational carrier. The molecular diagnostics apparatus of the embodiment has a simpler structure and better reliability.

Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and can be contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

Limit size lipid nanoparticles, methods for using the lipid nanoparticles, and methods and systems for making limit size lipid nanoparticles.

A disposable syringe for use with a pneumatic driver. The syringe has an elongated barrel with a cap secured to the proximal end that has a through bore. A filter is secured to the cap interior of the barrel that covers an opening in the through bore interior of the barrel. The pneumatic driver includes a source of pressurized air; a support for receiving and locating the syringe; and a supply block configured to receive pressurized air from the source and having an outlet for delivery of pressurized air. The supply block is movable between a first position spaced apart from the cap of the syringe and a second position in contact with the cap with fluid communication being established between the outlet of the supply block and the through-bore in the cap.

The present invention relates generally to the field of chemical and biological sensors and in particular to micro electro-mechanical systems (MEMS) sensors for measuring fluid viscosity and detection of minute amounts of chemicals and biological agents in fluids. It is an object of the present invention to provide a sensor that will work in disposable cartridges with remote sensing that can measure dynamic changes of the functionalized cantilevers in liquid and gas environment.

The present invention provides methods and devices for simple, low power, automated processing of biological samples through multiple sample preparation and assay steps. The methods and devices described facilitate the point-of-care implementation of complex diagnostic assays in equipment-free, non-laboratory settings.

The present disclosure provides a cartridge for use in nucleic acid extraction and a nucleic acid extraction method which can simplify a nucleic acid extraction process and can be applicable to a point-of-care testing (POCT). The cartridge includes a chamber module, an air valve module, and a liquid valve module. The chamber module includes a plurality of chambers for extracting nucleic acids from a sample including a pretreatment chamber in which the sample is crushed and homogenization, cell disruption, and purification are performed. The air valve module is installed on the chamber module to control a pressure required to move a fluid between the plurality of chambers. The liquid valve module is installed below the chamber module to move the fluid between the plurality of chambers.

A bench top incubator is described. The bench top incubator includes a first tray stack designed to retain microscope slides in a plurality of slide trays and a second tray stack designed to retain multi-well plates in a plurality of plate trays. The incubator is relatively simple and small in design and can be conveniently located to carry out temperature processing of biological samples such as fixed cells and tissues, biological fluids, and so forth.

A fluid sampler includes: a sample cell that includes: a substrate comprising: a first port; a second port in fluid communication with the first port; a viewing reservoir in fluid communication with the first port and the second port and that receives the fluid from the first port and communicates the fluid to the second port, the viewing reservoir including: a first view membrane; a second view membrane; and a pillar interposed between the first view membrane and second view membrane, the pillar separating the first view membrane from the second view membrane at a substantially constant separation distance such that a volume of the viewing reservoir is substantially constant and invariable with respect to a temperature and invariable with respect to a pressure to which the sample cell is subjected.