The present invention relates to a microfluidic device (100) for mixing liquids, wherein the microfluidic device (100) comprises a plurality of device inlets (110), each device inlet (110) for receiving a liquid; a chamber assembly (120) comprising a set of chamber inlets (122) in fluid communication with the device inlets (110); a mixing chamber (124) for receiving the liquids through the chamber inlets (122); and a plurality of chamber outlets (126) for communicating the liquids away from the mixing chamber (124); and a set of device outlets (130) in fluid communication with the chamber outlets (126), wherein the chamber outlets (126) are spaced around the mixing chamber (124) such that the mixing chamber (124) facilitates uniform mixing of the liquids communicating from the chamber inlets (122) to the chamber outlets (126). The invention also relates to a method of additive manufacturing a product comprising the microfluidic device as well as a liquid control system for controlling liquids in a microfluidic device.

A fluidic component intended to be associated with a heating module and wherein there is created a fluidic circuit which includes an inlet channel and an outlet channel, the fluidic component including a fluidic valve mechanism including: a fluidtight reservoir intended to be filled with a volume of gas capable of expanding, a deformable membrane closing the reservoir in a fluidtight manner, the membrane being able to deform by expansion of the volume of gas, between a first position wherein it forms a passage between the inlet channel and the outlet channel so as to allow a fluid to pass, and a second position wherein it obstructs the passage.

Disclosed is a pressure determining unit configured for determining a pressure of a fluid. The pressure determining unit comprises a body structure and a deformation detector. The body structure has a fluidic path configured for conducting the fluid, wherein the body structure has a first surface in a first dimension and in a second dimension, and a thickness in a third dimension. The deformation detector is configured for responding to an elongation into the second dimension of the first surface of the body structure by generating a signal indicative of a value of the pressure of the fluid in the body structure. The fluidic path of the body structure comprises one or more first channel segments, each first channel segment having a height into the third dimension being at least twice of its width into the second dimension.

The present disclosure provides a cartridge for use in nucleic acid extraction and a nucleic acid extraction method which can simplify a nucleic acid extraction process and can be applicable to a point-of-care testing (POCT). The cartridge includes a chamber module, an air valve module, and a liquid valve module. The chamber module includes a plurality of chambers for extracting nucleic acids from a sample including a pretreatment chamber in which the sample is crushed and homogenization, cell disruption, and purification are performed. The air valve module is installed on the chamber module to control a pressure required to move a fluid between the plurality of chambers. The liquid valve module is installed below the chamber module to move the fluid between the plurality of chambers.

A nucleic acid extraction system, comprising: a nucleic acid extraction plate, and a pipetting device and positive pressure device that are arranged side by side, the pipetting device comprising: an infusion device, an infusion device mounting frame, an infusion device driving unit, and a plurality of infusion units, each of the infusion units comprising: a reagent supply device and a reagent flow controller; the positive pressure device comprises: a plug, an air pipe, a positive pressure provider and a positive pressure driving device. The nucleic acid extraction system is highly efficiency and low-cost when extracting nucleic acid.

The present disclosure relates to devices, systems, and methods for modeling ocular translaminar pressure gradients ex vivo. A first fluid pressure level can be applied to a first side of a wall of donor eye cup (e.g., a posterior human eye cup) to simulate intracranial pressure (ICP), for example around the optical nerve head (ONH), and a second fluid pressure level can be applied to a second side of the wall of the donor eye cup to simulate intraocular pressure (IOP). These devices, systems, and methods are unique in that they allow ex vivo modeling of dynamic changes in translaminar pressure gradients.

A fluid control device includes an inlet configured to be placed directly or indirectly in fluid communication with a bodily fluid source and an outlet configured to be placed in fluid communication with a fluid collection device. The fluid control device has a first state in which a negative pressure differential produced from an external source such as the fluid collection device is applied to the fluid control device to draw an initial volume of bodily fluid from the bodily fluid source, through the inlet, and into a sequestration portion of the fluid control device. The fluid control device has a second state in which (1) the sequestration portion sequesters the initial volume, and (2) the negative pressure differential draws a subsequent volume of bodily fluid, being substantially free of contaminants, from the bodily fluid source, through the fluid control device, and into the fluid collection device.

A specimen chamber for transmitted light microscopy includes a chamber body having a specimen space that is sealed off in a transmitted light direction on opposite sides by transparent first and second observation windows, respectively, a seal being interposed in each case. First and second clamping elements are configured to fix the two observation windows to the specimen space. The clamping elements comprise observation openings into the specimen chamber. The first observation window comprises a first plane-parallel shoulder that protrudes into the first observation opening of the first clamping-element so as to fit exactly. The second observation window comprises a second plane-parallel shoulder that protrudes into the second observation opening of the second clamping element so as to fit exactly. The two seals are resistant to high pressure. The observation windows and the seals each consist of a plastomer.

Disclosed is an analysis system for analyzing water and wastewater, comprising an analysis device that includes a device housing which accommodates device components and which has an inlet on a housing surface, said inlet being designed as an injection port through which a substance to be analyzed can be introduced into a device component when the device housing is closed, and comprising a syringe that includes an injection needle outlet, the surface normal of which is congruent with the longitudinal axis; and/or the syringe includes an automatic ejection element for ejecting a predetermined amount of substance within a predetermined injection period.

Disclosed here are kits comprising pre-packed stabilizing solutions for stabilizing combinations of biomarkers at room temperature. Such kits can be better adapted for sample collection at a subject's dwelling, thus easing the burdensome requirement of sample collection.