A nucleic acid amplification reaction method includes subjecting a reaction mixture containing a nucleic acid amplification reaction reagent to be used for amplifying a nucleic acid to a thermal cycle for amplifying the nucleic acid, wherein in the thermal cycle, a heating time for an annealing reaction and an elongation reaction is 1 sec or more and 10 sec or less, the nucleic acid amplification reaction reagent contains a forward primer, a reverse primer, a polymerase, and a fluorescently labeled probe, the concentration of the forward primer is 0.4 μM or more and 3.2 μM or less, the concentration of the reverse primer is 0.4 μM or more and 3.2 μM or less, the amount of the polymerase is 0.5 U or more and 4 U or less, and the concentration of the fluorescently labeled probe is 0.15 μM or more and 1.2 μM or less.

A detection device for detecting a marker in a liquid, comprising a reaction chamber, provided with a thermosensitive sensor, wherein said reaction chamber comprises an photopolymer capable of releasing or generating a chemical species that is capable of undergoing or initiating an exothermic or endothermic chemical reaction with a marker present in the liquid.

Provided are methods and devices for the detection of bacteriophages.

The disclosed system and method concentrates and enriches a chemical sample while removing water and/or CO2 prior to analysis, improving detection limits and repeatability of quantitative chemical analysis without the need for cryogenic or sub-ambient cooling. The system can include a valve system, a dewpoint control zone, and a multi-capillary column trapping system (MCCTS). During a first time period, the valve system can couple the dewpoint control zone to the MCCTS. During a second time period, the valve system can couple the MCCTS to the chemical separation column such the dewpoint control zone is bypassed. Excess water included in the sample can condense in the dewpoint control zone as the sample transfers to the dewpoint control zone and MCCTS. When the sample is transferred from the MCCTS to the chemical separation column, the condensed water in the dewpoint control zone is not transferred to a chemical separation column.

A fluid analyzer manifold for facilitating flow of a fluid through at least one surface mounted component for analysis by a fluid analyzer. The exemplary fluid analyzer manifold can include an analysis chamber for connection with the fluid analyzer, a first flow channel having a first surface opening and a second flow channel having a second surface opening on the fluid analyzer manifold, and a mounting area on the fluid analyzer manifold. The mounting area can include the first and second surface openings of the first and second flow channels, and facilitates surface mounting the at least one surface mounted component to the fluid analyzer manifold.

The invention consists of a method for determining Total Dietary Fiber (TDF) and its sub-fractions, Insoluble Dietary Fiber (IDF) and Soluble Dietary Fiber (SDF) in food and feed samples which utilizes flexible reaction/filtration containers that can be divided into one or more sections for capturing the IDF and SDF fractions separately or for capturing TDF in its entirety. Each container is fashioned as a bag that can be temporarily sealed in multiple locations to create multiple sections and is made of non-porous and porous material. Use of these containers eliminates the need for problematic transfers of mixtures from beaker to filter, and vastly improves the filtration process.

A continuous throughput microfluidic system includes an input system configured to provide a sequential stream of sample plugs; a droplet generator arranged in fluid connection with the input system to receive the sequential stream of sample plugs and configured to provide an output stream of droplets; a droplet treatment system arranged in fluid connection with the droplet generator to receive the output stream of droplets in a sequential order and configured to provide a stream of treated droplets in the sequential order; a detection system arranged to obtain detection signals from the treated droplets in the sequential order; a control system configured to communicate with the input system, the droplet generator, and the droplet treatment system; and a data processing and storage system configured to communicate with the control system and the detection system.

Disclosed is a sample processing method for processing a target component in a sample by use of a sample processing chip having a storage portion and a droplet forming flow path, the sample processing method including: storing, in the storage portion, a mixture of the target component and a predetermined amount of a diluent for causing the target component to be encapsulated by one molecule or by one particle into a droplet; heating the mixture in the storage portion to cause thermal convection in the storage portion thereby to mix the target component and the diluent together; and in the droplet forming flow path, forming droplets in a dispersion medium, each droplet containing the diluted target component and a reagent that reacts with the target component.

A biological sample analysis system including a sample preparation system forming droplets of segmented sample separated by a carrier fluid immiscible with the sample. The droplets include reaction mixtures for amplification of at least one target nucleic acid. A thermal cycling device having a sample block having a plurality of controlled thermal zones, and a containment structure in thermal communication with the plurality of controlled thermal zones. The containment structure receives and contains the droplets of segmented sample separated by the immiscible carrier fluid from the sample preparation system. A controller for controlling a temperature in each thermal zone of the sample block. A detection system detects electromagnetic radiation emitted from each of the droplets individually from the queue of droplets as they flow past the detection system. A positioning system to facilitate moving a queue of the droplets in the thermal cycling device relative to the detection system.

A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block configured to receive the sample holder, a cover lid configured to move in a direction toward the sample block from an open position to a closed position, a heated cover operatively coupled to the cover lid and configured to move in a direction toward the sample block from a raised position to a first lowered position, in which the heated cover contacts the sample holder when the sample holder is received by the sample block, and a drive assembly including a motion guide configured to move in a direction toward the sample block from a first position, wherein the cover lid is in the open position and the heated cover is in the raised position, to a second position, wherein the cover lid is in the closed position.