A method for real-time quantitative detection of single-type, target nucleic acid sequences amplified using a PCR in a microwell, comprising introducing in the microwell a sample comprising target nucleic acid sequences, magnetic primers, and labelling probes; performing an amplification cycle to form labelled amplicons; attracting the magnetic primers to a surface through a magnetic field to form a layer including labelled amplification products and free magnetic primers; and detecting the labelled amplification products in the layer with a surface-specific reading method.

Systems and methods for light based heating of light absorbing sources for modification of nucleic acids through fast thermal cycling of polymerase chain reaction are provided. The system includes a polymeric fluidic device comprising one or more reaction wells. A first light absorbing material is disposed on a first support to define a reaction well and first and second ports are coupled to the reaction wells. The first and second ports are configured to allow input of a fluidic sample into the reaction well. A lyophilized reagent is pre-loaded in the reaction well. A light source is configured to illuminate the first light absorbing material. A first portion of light illuminated onto the first light absorbing material is absorbed into the first light absorbing material and is configured to elevate the temperature of the first light absorbing material to heat the fluidic sample within the reaction well.

Method of analysis. In the method, a microfluidic device defining a flow path extending from an inlet to an outlet may be selected. A sample-containing fluid may be introduced into the flow path via the inlet. Volumes of the sample-containing fluid may be isolated from one another on the flow path. A two-dimensional monolayer of the volumes may be imaged. The two-dimensional monolayer may be formed along the flow path between the inlet and the outlet.

The present disclosure relates to an automated assay system adapted to receive consumables in the conduct of an assay. The assay system includes at least one of a loading cart, a door support system, a waste storage unit, and a robotic subsystem. The loading cart is configured to organize and transport consumables necessary for the performance of the assay system. The door support system is configured to maintain stability of doors of the automated assay system. The waste storage unit is configured to provide convenient disposal of consumables associated with the automated assay system. The robotic subsystem is configured to transport consumables within the automated assay system.

The present disclosure provides microfluidic test platforms, systems, and methods for manufacturing the disclosed test platforms. The present disclosure further provides uses of the disclosed microfluidic test platforms in personalized medicine. Specifically, in providing prognostic and therapeutic methods for determining drug sensitivity and optimizing treatment regimen for subjects suffering from a pathologic disorder, specifically, cancer.

The present disclosure relates to a sample assessment device. By way of example, the sample assessment device may include a substrate including a sample application region; an amplification region comprising a plurality of amplification reagents; a waste region comprising an entrance fluidically coupled to the amplification region and extending away from the amplification region; and a detection region spaced apart from the amplification region. The sample assessment device may also include a valve coupled to the substrate and configured to separate the amplification region from the detection region in a closed configuration, wherein the amplification region and the valve are positioned on the sample assessment device between the sample application region and the detection region and wherein the sample assessment device is configured to permit lateral flow from the amplification region to the detection region when the valve is in an open configuration.

Disclosed is a system with (a) a microplate comprising a metallic material for heating one or more samples during thermocycling. The microplate comprises one or more barcodes on a side of the microplate. The system may comprise a barcode reader for reading the one or more barcode. The system may also comprise a first temperature block configured to receive the microplate and/ or provide a denaturing temperature to the one or more samples contained in the microplate, during the thermocycling. The system may also comprise a second temperature block configured to receive the microplate and/or provide an extension temperature to the one or more samples contained in the microplate, during the thermocycling. The system may also comprise a conveyor configured to move the microplate between a sensing device, the first temperature block, and the second temperature block, during the thermocycling.

Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid together with a target compound having a water solubility equal to or greater than 0.01 mg per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes.

A housing for a simulation chamber having a chamber body, a door arranged on the chamber body, and a controller housing, wherein the controller housing is arranged on the chamber body and comprises an outer surface, an inner surface, and at least one side surface, in that the door comprises an outer surface, an inner surface, and at least five side surfaces, wherein the controller housing is arranged on the chamber body in such a manner that, when the door is closed, at least one of the side surfaces of the door is arranged facing at least one of the side surfaces of the controller housing.

A test microfluidic strip or cartridge comprising a blood sample collection zone, red blood cell isolation zone, lysis zone, reagents mixing zone, and sensing zone for measuring the enzymatic activity thiopurine methyltransferase is disclosed. The test strip or cartridge is disclosed to be operably connected to a metering device comprising a processor, a display and a memory card.