The invention provides devices for generating pre-templated instant partitions. The devices may include a shearing mechanism, such as a vortexer, a holder for holding a vessel containing a liquid onto the vortexer, and a temperature control unit for modulating a temperature of the vessel by convection. The invention also provides methods of using such devices to process analyte inside the pre-templated instant partitions.

A microfluidic device for non-invasively and passively accessing interstitial fluid from a patient includes a substrate containing multiple vertical micro channels therethrough, wherein at a first end of each of the multiple vertical micro channels a microheater is formed for controllably ablating a portion of dry dead skin cells to access the interstitial fluid; and wherein at a second end of each of the multiple vertical micro channels is a horizontal micro channel for receiving accessed interstitial fluid from a vertical micro channel and guiding the accessed interstitial fluid to a common collection port.

A sensing chip with fluidic device includes a substrate with a first area and a second area, a field effect transistor is arranged in the second area of the substrate and is electrically connected with the field effect transistor. The fluidic device includes an insulation layer with a window to expose the surface of substrate in the second area. A second gate electrode is arranged in the window of the isolation layer on the second area of the substrate. The sample is placed in the fluidic device to contact with the second gate electrode, and the receptor(s) on the metal layer will capture the target object in the sample, so the voltage of the metal layer will change with amount of the target object captured by the receptor(s). Thus, the concentration of the target object(s) in the sample may obtain by the changes of the voltage of the metal layer.

A reaction processor includes: a reaction processing vessel including a channel in which a sample moves and a pair of air communication ports, a first air communication port and a second air communication port, provided at respective ends of the channel; a temperature control system that provides a medium temperature region and a high temperature region between the first air communication port and the second air communication port in the channel; and a liquid feeding system that discharges and sucks air in order to move and stop the sample inside the channel. One of the pair of air communication ports of the reaction processing vessel that is farther away from the high temperature region communicates with the liquid feeding system via a tube. One of the pair of air communication ports of the reaction processing vessel that is closer to the high temperature region is opened to atmospheric pressure.

An assembly for forming a microchamber for an inverted substrate is disclosed. The assembly can include a body having a chamber formed therein. A dispensing cavity can be provided to supply a reagent to the chamber. A slide support structure can be configured to support the slide such that the tissue sample faces the chamber when the slide is mounted to the slide support structure. The chamber and the slide support structure can be dimensioned such that, when the reagent is supplied to the dispensing cavity, the reagent is drawn to the chamber by way of capillary forces acting on the reagent.

Systems, methods, and apparatuses are provided for self-contained nucleic acid preparation, amplification, and analysis.

The purpose of the present invention is to provide a novel digital PCR analysis method. In the digital PCR analysis method disclosed herein, a method for detecting DNA is used, which includes the steps of: dividing a DNA solution containing a fluorescent-labeled probe or a DNA intercalator and a plurality of DNAs to be detected into a plurality of compartments; carrying out PCR in the compartments; measuring a fluorescence intensity in association with a change in temperature; calculating a melting temperature from a melting curve for a DNA double strand measured on the basis of a change in fluorescence intensity, which is associated with the change in temperature; and calculating a temperature difference between two points with a slope of a predetermined value on a melting curve indicating a change in the fluorescence intensity.

Provided herein are microfluidic devices for analyzing samples. In one aspect, the microfluidic device includes a body structure having a droplet compression chamber, a sieve structure in fluid communication with the droplet compression chamber, which sieve structure comprises an array of protrusions that extend from at least one surface of the body structure and define at least a portion of one or more fluidic circuits, and a port at least partially disposed in the body structure. Other aspects include kits, methods, systems, computer readable media, and related aspects for analyzing samples.

The present disclosure relates to a flow cell receiver. The flow cell receiver can include at least one platen, having a plurality of ports. The flow cell receiver can include magnets. The flow cell receiver can be configured to automatically align, secure, and retain a flow cell carrier containing a flow cell.

The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.