A fluidic system that includes a reagent manifold comprising a plurality of channels configured for fluid communication between a reagent cartridge and an inlet of a flow cell; a plurality of reagent sippers extending downward from ports in the manifold, each of the reagent sippers configured to be placed into a reagent reservoir in a reagent cartridge so that liquid reagent can be drawn from the reagent reservoir into the sipper; at least one valve configured to mediate fluid communication between the reservoirs and the inlet of the flow cell. The reagent manifold can also include cache reservoirs for reagent re-use.

A method and system are provided for extracting a target analyte from a sample using acoustic ejection technology. The method involves applying focused acoustic energy to a fluid reservoir housing a fluid composition that contains a target analyte and comprises an upper region and a lower region, where the concentration of the target analyte in the upper region differs from that in the lower region. The focused acoustic energy is applied in a manner that is effective to result in the ejection of a fluid droplet from the fluid composition into a droplet receiver, wherein the concentration of the analyte in the droplet corresponds to either the concentration of the analyte in the upper region or the concentration of the analyte in the lower region, and wherein the concentration of the analyte is substantially uniform throughout the droplet. The fluid composition may comprise an ionic liquid, used in the extraction of ionic target analytes. Related methods and an acoustic extraction system are also provided.

A droplet generating apparatus, system, and method are disclosed. Based on a relative vibration between a micro-pipe and a container containing a second liquid, a first liquid flowing out from an outlet end of the micro-pipe can be detached from the micro-pipe by a fluid shear force of the second liquid to form droplets in the second liquid. Multitudinous quantitative and uniform droplets can be generated precisely and accurately in the present disclosure.

A fluid handling device has an introduction port, a first flow channel which is connected to the introduction port and in which a droplet can move when a fluid including the droplet is caused to flow therein, a first chamber for capturing the droplet moving through the first flow channel, and a second chamber through which the droplet captured by the first chamber can move via the first flow channel. The liquid handling device is capable of switching between a first state in which a droplet moving through the first flow channel is captured by the first chamber, and a second state in which the droplet captured by the first chamber moves to the second chamber via the first flow channel.

Provided are microfluidic systems and methods for detecting, sorting, and dispensing of low abundance events such as single cells and particles, including a variety of eukaryotic and bacterial cells, for a variety of bioassay applications. The systems and methods described herein, when implemented in whole or in part, will make relevant microfluidic based tools available for a variety of applications in biotechnology including antibody discovery, immuno-therapeutic discovery, high-throughput single cell analysis, target-specific compound screening, and synthetic biology screening.

The present invention relates to a loading well (320) comprising a lateral wall part (3211) in cross-section parallel to the base plan (x/y) and/or a bottom wall part comprising at least one sloped bottom section (32121). The present invention also relates to a microfluidic chip comprising the same; systems comprising the same configured to reduce the dead volume of a drop of sample to be loaded in the microfluidic chip and/or to trap a drop in a defined location; and methods using the same.

The present disclosure provides a microfluidic chip and a droplet separation method, and belongs to the field of biological chip technology. The microfluidic chip includes a first liquid tank and a second liquid tank opposite to each other and a channel layer therebetween. The channel layer includes a plurality of microfluidic channels separated from each other, first ends of the microfluidic channels are communicated with the first liquid tank, and second ends are communicated with the second liquid tank. The first liquid tank contains sample solution to be detected, and the second liquid tank contains encapsulating liquid. The sample solution to be detected entering the first liquid tank may be separated into a plurality of sample droplets through the microfluidic channels, the separated sample droplets enter the second liquid tank, so that the encapsulating liquid is encapsulated on a surface of each of the plurality of sample droplets.

The present disclosure relates to a microfluidic chip and a control method thereof, a droplet generation device and a microsphere preparation device. The microfluidic chip includes a matrix (3), and a first flow channel (1) and a second flow channel (2) provided in the matrix (3), wherein the first flow channel (1) and the second flow channel (2) intersect to form an intersection area, sheared phase fluid can flow in from the first flow channel (1), shearing phase fluid can flow in from the second flow channel (2) so as to separate the sheared phase fluid into discrete droplets in the intersection area, and the cross-sectional areas of the first flow channel (1) and the second flow channel (2) range from 0.1 mm.sup.2 to 1 mm.sup.2. The microfluidic chip can increase the flow rate and improve the efficiency of forming droplets; and the efficiency of generating the droplets is increased on the basis of ensuring the cell activity in order to meet the requirements of 3D biological printing.

A microfluidic apparatus for delivering droplets of a first fluid to droplets of a second fluid, comprising a main channel, with a carrier fluid carrying droplets of the second fluid, an auxiliary channel, fluidly coupled to the main channel at a first intersection via a first orifice with a first fluid interface, and at a second intersection downstream to the first intersection via a second orifice with a second fluid interface, wherein a flow of the carrier fluid induces a difference of pressure between the first and second orifice generating a balance condition such that a meniscus of the second fluid interface is maintained in the auxiliary channel, at the vicinity of the second orifice, wherein a balance deviation triggers a release of a volume of the first fluid from the second fluid interface into the main channel.

A method and system are provided for extracting a target analyte from a sample using acoustic ejection technology. The method involves applying focused acoustic energy to a fluid reservoir housing a fluid composition that contains a target analyte and comprises an upper region and a lower region, where the concentration of the target analyte in the upper region differs from that in the lower region. The focused acoustic energy is applied in a manner that is effective to result in the ejection of a fluid droplet from from the fluid composition into a droplet receiver, wherein the concentration of the analyte in the droplet corresponds to either the concentration of the analyte in the upper region or the concentration of the analyte in the lower region, and wherein the concentration of the analyte is substantially uniform throughout the droplet. The fluid composition may comprise an ionic liquid, used in the extraction of ionic target analytes. Related methods and an acoustic extraction system are also provided.