The present invention provides a medical device and a method for the selective separation of a biological sample of a mammal into a first portion and a second portion. It comprises a first layer comprising a first reservoir for receiving the biological sample and for retaining the first portion of the sample, and a second layer comprising a second reservoir for receiving the second portion of the sample. Between the first layer and the second layer a third layer is provided, wherein the third layer comprises a plurality of channels configured to provide a fluid communication between the first reservoir and the second reservoir. Furthermore, between the first layer and the second layer a fourth layer adjacent to the third layer is provided, wherein the fourth layer comprises and/or is configured as a separation layer. At least the third and fourth layer are configured to selectively separate the biological sample between the first reservoir and the second reservoir into the first portion and the second portion of the sample. According to the invention, the layers are to be understood to be stackable in a substantially vertical plane, forming a three-dimensional layered structure.

Fluid processing assemblies and methods are provided for mononuclear cell collection. Mononuclear cells are separated from red blood cells in a blood separation chamber, with the mononuclear cells and then the red blood cells exiting the chamber via an outlet port. The mononuclear cells and then the red blood cells enter an outlet flow path that is in fluid communication with a mononuclear cell collection container. The outlet flow path includes a visual indicium, which an operator may use to determine the position of the red blood cells within the outlet flow path and when to end mononuclear cell collection by preventing fluid communication between the outlet flow path and the mononuclear cell collection container.

A method of performing a separation of a sample of a disperse fluid comprises the steps of: i. providing a sample of a disperse fluid comprising particles dispersed in a fluid, wherein the particles comprises at least a first type of particle and at least a second type of particles, wherein the absolute value of the acoustic contrast of the first type of particle, relative to the fluid, is lower than the absolute value of the acoustic contrast of the second type of particle relative to the fluid, and wherein the first and second type of particle either both have a positive acoustic contrast, or alternatively a negative acoustic contrast, relative to the fluid, ii. positioning the sample in a microfluidic cavity, iii. subjecting the sample, in the microfluidic cavity, to an acoustic standing wave configured for causing the first and second type of particle to congregate in at least one first region of the cavity, thereby causing the fluid to occupy at least one second region of the cavity, and thereby defining at least one interface between the first region and the second region, and iv. collecting at least a portion of the first region adjacent and along the at least one interface to obtain the first type of particles. A system is also disclosed.

Provided herein is a semen processing device. The device is a collection cup with a top portion encompassing a collection cavity into which the semen sample is deposited and a bottom portion encompassing a processing chamber and containing the processing unit for the collected semen. A flow control valve or a serpentine channel may be disposed as part of the processing unit to regulate the flow rate of semen from the collection cavity into the processing unit. A harvesting dock is disposed through the surface of the bottom portion. The semen flows from the collection cavity into the processing unit and is harvested through the harvesting dock.

A bioprocessing filtration experiment system for filtering a liquid test medium as part of a filtration experiment in a filtration experiment section of the filtration experiment system, which filtration experiment section runs from a receptacle for holding the test medium to be filtered to a fluid outlet for the filtered test medium, wherein the filtration experiment system is designed to ascertain, as part of the filtration experiment, sensor data as experiment data for at least one filter, said experiment data being able to be taken as a basis for selecting and/or dimensioning the filter of a target system according to predetermined scaling criteria. It is proposed that the filtration experiment system can be preassembled on an at least partially programming-related and/or circuit-related, at least partially fluidics-related and/or at least partially sensor-related basis.

Disclosed herein are microfluidic devices and methods to deliver concentration gradients to biological material such as oocytes and embryos for the purpose of cryopreparation, cryopreservation, or thawing. Cryopreservation methods, such as vitrification, involve the use of cryoprotectants to reduce formation of damaging ice crystals in cells during freezing. Microfluidic devices and methods described herein improve cell viability and efficiency during handling and cryopreservation of biological materials.

A technique relates to a fluidic cell configured to hold a nanofluidic chip. A first plate is configured to hold the nanofluidic chip. A second plate is configured to fit on top of the first plate, such that the nanofluidic chip is held in place. The second plate has at least one first port and at least one second port. The second plate has an entrance hole configured to communicate with an inlet hole of the nanofluidic chip. The second port is angled above the first port, such that the first port and second port intersect to form a junction. The second port is formed to have a line-of-sight to the entrance hole, such that the second port is configured to receive input for extracting air trapped at a vicinity of the entrance hole.

Systems for processing a fluid sample to facilitate analysis with a semiconductor detection chip are provided herein. Such systems can include a sample processing cartridge coupleable with a chip carrier device configured for transport of the processed fluid sample from the sample cartridge. The chip carrier device can include one or more fluid channels extending between fluid-tight couplings attachable to transfer ports of the sample processing cartridge. The chip carrier device can include multiple portions or adapters, including a fluid sample portion, a flowcell portion and a chip carrier. Also provided are methods of preparing and transporting a fluid sample from a sample cartridge into a chip carrier device for analysis with a semiconductor detection chip carried within the chip carrier device.

Described here are systems and methods for a reusable, aseptic connector. A connector system provides aseptic fluid connection. The connector system has an inlet connector assembly having an inlet fluid passageway and an inlet valve configured to seal the inlet fluid passageway when in a disconnected state. The connector also has an outlet connector assembly having an outlet fluid passageway and an outlet valve configured to seal the outlet fluid passageway when in a disconnected state. The inlet valve is configured to couple to the outlet valve to form an aseptic fluid connection between the inlet and outlet fluid passageways when in a connected state.

A pipetting device includes a housing, a valve assembly, and an actuator assembly. The housing includes a tip for receiving a capillary tube. The valve assembly includes a shuttle valve having a filling position and a dispensing position and a valve rod. The actuator assembly includes an actuator, a valve trigger, a piston mount, and an indexing mechanism. The actuator extends from the housing and has a push button coupled to a push rod. The valve trigger being configured to engage the valve assembly and includes an aperture configured to receive the piston mount therein. The indexing mechanism is configured to index a rotated position of the push button to a predefined volume of dispensed fluid.