In one example, a flow cell includes a plurality of inlet ports sized to receive a flow of reagent from one of a plurality of reagents into the flow cell. An outlet port of the flow cell is sized to pass each flow of reagent out of the flow cell. A flow channel of the flow cell is positioned between, and in fluid communication with, each inlet port and the outlet port. The flow channel includes a manifold section and a detection section. The manifold section has a plurality of manifold branches in fluid communication with a common line, wherein each branch is connected to one of each inlet port. The detection section is in fluid communication with the common line and the outlet port. The detection section is operable to perform a plurality of different chemical reactions between the plurality of reagents and analytes positioned in the detection section.

A specimen mixing and transfer device adapted to receive a sample is disclosed. The specimen mixing and transfer device includes a housing, a material including pores that is disposed within the housing, and a dry anticoagulant powder within the pores of the material. In one embodiment, the material is a sponge material. In other embodiments, the material is an open cell foam. In one embodiment, the material is treated with an anticoagulant to form a dry anticoagulant powder finely distributed throughout the pores of the material. A blood sample may be received within the specimen mixing and transfer device. The blood sample is exposed to and mixes with the anticoagulant powder while passing through the material.

A specimen mixing and transfer device adapted to receive a sample is disclosed. The specimen mixing and transfer device includes a housing, a material including pores that is disposed within the housing, and a dry anticoagulant powder within the pores of the material. In one embodiment, the material is a sponge material. In other embodiments, the material is an open cell foam. In one embodiment, the material is treated with an anticoagulant to form a dry anticoagulant powder finely distributed throughout the pores of the material. A blood sample may be received within the specimen mixing and transfer device. The blood sample is exposed to and mixes with the anticoagulant powder while passing through the material.

An apparatus includes a flow cell body, a plurality of electrodes, an integrated circuit, and an imaging assembly. The flow cell body defines one or more flow channels and a plurality of wells. Each flow channel is configured to receive a flow of fluid. Each well is fluidically coupled with the corresponding flow channel. Each well is configured to contain at least one polynucleotide. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are operable to effect writing of polynucleotides in the corresponding wells. The integrated circuit is operable to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells to thereby generate polynucleotides representing machine-written data in the wells. The imaging assembly is operable to capture images indicative of one or more nucleotides in a polynucleotide.

A specimen transfer device adapted to receive a blood sample is disclosed. The specimen transfer device includes a housing and an actuation member. A deformable material is disposed within the housing and is deformable from an initial position in which the material is adapted to hold the sample to a deformed position in which at least a portion of the sample is released from the material. A viscoelastic member is disposed within the housing between the material and the housing and between the material and the actuation member. The viscoelastic member is engaged with the actuation member and the material such that movement of the actuation member from a first position to a second position deforms the material from the initial position to the deformed position.

Example embodiments relate to fast sample loading microfluidic reactors and systems. One embodiment includes a microfluidic device. The microfluidic device includes a reaction chamber allowing reacting of at least one fluid material. The microfluidic device also includes at least two fluidic channels coupled to the reaction chamber for providing a fluid to and exiting a fluid from, respectively, the reaction chamber. Each fluidic channel includes an inlet and an outlet. Each fluidic channel is configured such that when a first fluid is provided in the reaction chamber via that fluidic channel, the first fluid exits the reaction chamber via the outlet of at least one other fluidic channel when the reaction chamber is filled, thereby preventing a second fluid from the at least one other fluidic channel, when present in the inlet, from diffusing into the reaction chamber.

The present invention provides a microchannel structure loaded with a bead having a particle size for detecting whether a biological substance exists in a sample. The microchannel structure includes a structure body for passing a sample through the microchannel structure to have a test or a treatment. The structure body includes a sample entrance having a first aperture to allow the sample passing therethrough, a resistance-increasing section connected with the sample entrance, and having a second aperture being smaller than the first aperture, a detecting section connecting with the resistance-increasing section, and a bead mooring structure coupled to the second end for mooring the bead in the detecting section. The present invention can be used to capture rare cells in a biological system, such as human blood.

A microfluidic device (100) comprises: a reaction chamber (102); at least a first and a second supply channel (110a, 110b) for allowing transport of a first fluid and a second fluid, respectively, from a fluid supply source (112a, 112b) into the reaction chamber (102), wherein each of the first and the second supply channels (110a, 110b) comprises a side drain (114a, 114b) connected to the supply channel (110a, 110b) between the fluid supply source (112a, 112b) and the reaction chamber (102), wherein the side drain (114a, 114b) is configured to prevent undesired diffusion of the fluid in the supply channel (110a, 110b) into the reaction chamber (102); at least a first and a second outlet (120a, 120b) connected to the reaction chamber (102) for allowing transport of fluid from the reaction chamber (102), wherein the first and second outlets (120a, 120b) have different dimensions to provide different hydraulic resistance.

A microfluidic biochip for detecting disease antigens using gold nano interdigitated electrode circuit under a controlled self-driven flow condition is disclosed. The biochip incorporates hydrophilic microchannels for controlled self-driven flow and gold nano interdigitated electrodes for capacitive sensing with enhanced sensitivity. The biochip's microchannel has a surface treated with oxygen plasma to control microchannel surface hydrophilicity and flow rate of the biofluid sample. Carbon Nanotubes (CNTs) are utilized as an intermediate layer to enhance the binding capability to nano electrodes to enhance sensitivity. Due to the carboxylic groups of the CNTs, covalent bond binding between the antibodies and the CNTs allows the antibodies to adhere more readily on the surface of the electrodes. The quantity of antibodies attaching to the surface is increased due to the high surface to area ratio in CNTs.

An apparatus includes a flow cell body, a plurality of electrodes, an integrated circuit, and an imaging assembly. The flow cell body defines one or more flow channels and a plurality of wells. Each flow channel is configured to receive a flow of fluid. Each well is fluidically coupled with the corresponding flow channel. Each well is configured to contain at least one polynucleotide. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are operable to effect writing of polynucleotides in the corresponding wells. The integrated circuit is operable to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells to thereby generate polynucleotides representing machine-written data in the wells. The imaging assembly is operable to capture images indicative of one or more nucleotides in a polynucleotide.