Patent classifications
B03C1/002
Systems and methods for high gradient magnetic separation of biological material
Described herein are methods for purification of biological material using high gradient magnetic separation. For example, a method includes: providing a ferromagnetic matrix surrounded by a separation column, the separation column including an elongate body defining a lumen having an inlet and an outlet; applying an external magnetic field to the separation column; saturating unspecific binding sites in the ferromagnetic matrix by applying a buffer solution to the ferromagnetic matrix; and introducing biological material into the lumen of the separation column. In some embodiments, the ferromagnetic matrix is uncoated, and the buffer solution includes at least one macromolecule. In some embodiments, the method further includes incubating the ferromagnetic matrix with the buffer solution for at least three minutes to equilibrate the ferromagnetic matrix.
Preparing antigen-specific T cells using a self-enclosed processing system that contains both a centrifuge and a magnetic separation column
The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.
Centrifuge and method for loading and centrifuging a reaction vessel unit
A centrifuge for cleaning a reaction vessel unit, having a rotor for holding at least one reaction vessel unit with its opening(s) directed outwardly, a motor for rotating the rotor around a rotation axis, and a loading mechanism with a beam for loading and unloading the centrifuge with the at least one reaction vessel unit.
Centrifuge including a magnetic element and method for centrifuging a reaction vessel unit
A centrifuge for cleaning a reaction vessel unit, having a rotor for holding at least one reaction vessel unit with its opening(s) directed outwardly, a motor for rotating the rotor around a rotation axis, a housing having a substantially cylindrical inner surface, wherein a drain is provided for discharging fluid expelled from the reaction vessel unit, wherein a gap is provided between the inner surface and the rotor so that by rotating the rotor a wind is generated which drives the expelled fluid on the inner surface to the drain wherein an aspiration pump is connected to the drain for discharging fluid.
Devices and disposables for patient-specific cell therapy manufacturing
The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.
Method for integrated processing of finely dispersed metal-containing waste
A method for integrated processing of finely dispersed metal-containing waste, wherein the method includes mixing metal-containing waste with magnetic field activated water into a pulp, subjecting the pulp in a reaction chamber to a rotating magnetic field generated by rotating ferromagnetic elements, carried out in a vortex layer with a rotation speed of the ferromagnetic elements being at least 2800 rpm, until a magnetostriction effect and subsequent reduction of metal oxides occurs, and carrying out hydrocyclone separation of the metal oxides.
CENTRIFUGE AND METHOD FOR CENTRIFUGING A REACTION VESSEL UNIT
A centrifuge for cleaning a reaction vessel unit, having a rotor for holding at least one reaction vessel unit with its opening(s) directed outwardly, a motor for rotating the rotor around a rotation axis, a housing having a substantially cylindrical inner surface, wherein a drain is provided for discharging fluid expelled from the reaction vessel unit, wherein a gap is provided between the inner surface and the rotor so that by rotating the rotor a wind is generated which drives the expelled fluid on the inner surface to the drain wherein an aspiration pump is connected to the drain for discharging fluid.
LITHIUM COMPOUND, METHOD FOR PRODUCING THE LITHIUM COMPOUND, AND METHOD FOR PRODUCING POSITIVE ACTIVE MATERIAL FOR NONAQUEOUS ELECTROLYTE SECONDARY BATTERY
A lithium compound is provided, with which a positive active material containing a decreased amount of magnetically attractable substances can be easily obtained while shortening the total time for production of a positive electrode for a nonaqueous electrolyte secondary battery. The lithium compound is used for producing a positive active material for a nonaqueous electrolyte secondary battery, with which a lithium transition metal composite oxide can be obtained by mixing the lithium compound with a transition metal composite hydroxide or the like obtained by crystallization reaction, and then firing the mixture obtained by mixing, wherein when water and a magnet are added to and mixed and stirred with the lithium compound, the amount of magnetically attractable substances captured by the magnet is 0.0007 mass % or less with respect to the amount of lithium in the lithium compound. Therefore, a positive active material in which the amount of magnetically attractable substances contained is 0.02 mass ppm or less can be easily obtained while shortening the total time for production of the positive active material.
METHOD FOR PRODUCING NANOMAGNETITE
The application relates to a method for preparing magnetite, comprising steps of:
a) reaction at a temperature of 100 to 500 C. of a material containing wstite with water, in order to obtain a solid comprising magnetite, and then
b) recovery of the magnetite in the form of particles wherein more than 25% by weight are of nanometric size.
CENTRIFUGE AND METHOD FOR CENTRIFUGING A REACTION VESSEL UNIT
A centrifuge for cleaning a reaction vessel unit, having a rotor for holding at least one reaction vessel unit with its opening(s) directed outwardly, a motor for rotating the rotor around a rotation axis, a housing having a substantially cylindrical inner surface, wherein a drain is provided for discharging fluid expelled from the reaction vessel unit, wherein a gap is provided between the inner surface and the rotor so that by rotating the rotor a wind is generated which drives the expelled fluid on the inner surface to the drain wherein an aspiration pump is connected to the drain for discharging fluid.