Provided are a fluorescent testing system, a dielectrophoresis device, and a molecular testing method that measure only fluorescence emitted from a test object without separating excitation light and the fluorescence by an optical filter and that are able to prevent reduction of an application range of a type of the fluorescence. A fluorescent testing system (1) includes: an excitation light source (23) that radiates excitation light (L1) to a test object (M) flowing in a microfluidic channel (22); a silicon integrated circuit (10) provided with a photon detection unit (13) that detects light by a photodiode (12); a dielectrophoresis electrode pair (16) that generates an electric field (EF) to draw the test object (M) onto the photodiode (12) by dielectrophoresis; and a control unit (24) that causes the excitation light source (23) to radiate the excitation light (L1) to the test object (M) that is drawn and causes the photon detection unit (13), after extinguishment of the excitation light (L1), to detect fluorescence (L2) emitted from the test object (M).

Embodiments of the present invention relate to a method for using a device for the positioning of molecules, the devise including a semiconductor substrate including a semiconductor layer and an insulating layer with a plurality of electrodes arranged on the insulating layer forming an electrode layer with a layer of 2-dimensional material arranged on the electrode layer. The method includes applying a first set of control signals to the plurality of electrodes to position a plurality of molecules in a first molecule arrangement and applying a second set of control signals to the plurality of electrodes to position the plurality of molecules in a second molecule arrangement, wherein the second set of control signals is different from the first set of control signals and wherein the device provides a first functionality in the first molecule arrangement and a second functionality in the second molecule arrangement.

In example implementations, an apparatus is provided. The apparatus includes a dielectrophoresis (DEP) separator, an electrical field generator, a tracking system, and a controller. The DEP separator is to separate a plurality of different particles. The electrical field generator is coupled to the DEP separator to apply a frequency to the DEP separator. The tracking system is to track a movement of a type of particles in the DEP separator. The controller is in communication with the electrical field generator to control the frequency and the tracking system to track the separation. The controller is to calculate a cross-over frequency from a cross-over frequency distribution for the type of particles based on a frequency sweep performed on the type of particles and the movement of the type of particles that is tracked.

A method and system for determining a concentration of one or more analytes in whole blood is provided. In one aspect of the invention, the system includes a channel configured to carry whole blood. The system further includes a light source configured to emit light on the channel. Additionally, the system includes an actuation module associable with the channel, wherein the actuation module is configured to generate a cell-free plasma layer in the channel. Furthermore, the system includes an optical module associable with the channel.

In a detection method, first dielectric particles each capable of being bound to a first target substance and second dielectric particles each capable of being bound to a second target substance are caused to react with a sample that contains a first target substance and a second target substance, the second dielectric particles having a different dielectrophoretic property from the first dielectric particles, a first composite particle to which the first target substance is bound is separated from the other first dielectric particle, and a second composite particle to which the second target substance is bound is separated from the other second dielectric particle by causing dielectrophoresis in the sample after the reaction, and the first target substance contained in the separated first composite particle and the second target substance contained in the second composite particle are each detected.

A microfluidic system which enables singular confinement of cells at the capturing stations and impedance measurements of single cells at these stations. The microfluidic system includes an inlet, a dielectrophoretic separation site, a waste outlet I, a connection pad, a hydrodynamic flow resistance. Collective measurements can also be obtained by measuring up to twenty singular cells at capturing stations simultaneously.

A method of sampling and testing for SARS-COV-2 virus in nasal and nasopharyngeal fluid using a plurality of microfluidic channels with a plurality of integrated electrodes in the microfluidic channels to detect the virus. In one example embodiment, a plurality of antibodies are fixed on a surface of at least one electrode by positive dielectrophoresis that increases the sensitivity of detection. Viral antigens bind to the antibodies separating from the fluid thereby signally that the virus is present as evidenced by the detection of the antigens. Sampling by microfluidic channels is more comfortable to a patient because microfluidic channels are soft, flexible and narrow compared to swabs. Another example embodiment of a method using microfluidic channels for collecting tears or saliva to determine blood glucose levels using a smartphone that has been modified to incorporate external filters quantitate glucose levels is also described.

A method for continuously separating components from a sample includes providing a field-flow fractionation device including: a channel coupled to a flow generator for translocating the sample components along the channel in a first direction, an actuator for translocating the sample components in a second direction, at an angle with the first direction, and an array of electrodes electrically or capacitively connected to an AC power source, operating the actuator so as to translocate the sample components in a second direction at an angle with the first direction, operating the AC power source so as to generate an AC electric field between adjacent rows, and operating the flow generator, collecting sample components from the sample outlets.

A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.

The present disclosure describes methods, devices and systems comprising materials comprising dielectrics. In various aspects, electrodes layered or imbedded with these dielectrics provide enhanced properties for a wide range of applications, such as the enhanced separation of analytes, such as biological molecules or particles (nucleic acids, viruses) with an electrokinetic field.