A fluid entrained particle separator may include an inlet passage to direct particles entrained in a fluid, a first separation passage branching from the inlet passage, a second separation passage branching from the inlet passage and electrodes to create electric field exerting a dielectrophoretic force on the particles to direct the particles to the first separation passage or the second separation passage, wherein the first separation passage, the second separation passage, the electric field and the dielectrophoretic force extend in a plane.

An object trapping device enables efficiently trapping a plurality of objects in a specific combination. Each of a first electrode pair (13), a second electrode pair (14), and a third electrode pair (15) in an electrode pair group (3) is applied with an individual AC voltage and traps an object by dielectrophoresis generated in accordance with the AC voltage that is applied.

Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

An interdigitated electrode biosensor includes an insulating layer configured to fully cover a sensor forming region of a substrate, a first interdigitated microelectrode configured such that a plurality of first protruding electrodes is arranged in a shape of a comb on the substrate, a second interdigitated microelectrode configured such that a plurality of second protruding electrodes is arranged in a shape of a comb and each interdigitates with the plurality of first protruding electrodes, and a plurality of receptors that is immobilized in a space between the first interdigitated microelectrode and the second interdigitated microelectrode and reacts specifically to target biomaterials. Different voltages are uniformly or nonuniformly applied to the first interdigitated microelectrode and the second interdigitated microelectrode to generate a dielectrophoretic force by a nonuniform electric field, improving the sensor by increasing the probability of specific reaction with the target biomaterials using the concentration effect through dielectrophoresis.

Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

A biosensing chip is provided, including a substrate having a photoelectric conversion material, and an electrode disposed on the substrate and including two contact portions and an electrode pattern, wherein the photoelectric conversion material is a monocrystalline silicon material, and the electrode pattern includes micro-electrodes in the form of interdigitated sawtooth. The biosensing chip and the method using the same may distinguish a lesion site of cancer cells and the degree of cancer lesions.

A microchamber array device having built-in reaction microchambers, in which the dilution ratio can be greatly increased at the same time as dramatically raising cell recovery efficiency, and an inspection object analysis method using said device are provided. This microchamber array device is provided with: a microchamber array 1 for cell capture by electrophoresis comprising an arrangement of a substrate 2, electrodes 3 and photoresists 4; and a reaction microchamber array 6 which is separated from the capture microchamber array 1, and which is formed from reaction microchamber 8 comprising micro channels 7 arranged so as to be opposite of the aforementioned microchamber array 1.

Microfluidic devices in which electrokinetic mechanisms move droplets of a liquid or particles in a liquid are described. The devices include at least one electrode that is optically transparent and/or flexible.

A target substance detection method includes forming a complex by causing a target substance and a dielectric particle to bind to each other, the dielectric particle being modified with a substance having a property of specifically binding to the target substance; separating the complex and an unbound particle from each other in a liquid by dielectrophoresis, the unbound particle being a dielectric particle not constituting the complex; and detecting the target substance included in the separated complex by using an imaging element.

A target substance detection method includes forming a complex by causing a target substance and a dielectric particle to bind to each other, the dielectric particle being modified with a substance (for example, an antibody) having a property of specifically binding to the target substance; subjecting a bound particle and an unbound particle to dielectrophoresis in a liquid, the bound particle being the dielectric particle constituting the complex, the unbound particle being a dielectric particle not constituting the complex; and detecting the target substance in the complex, based on a difference in motion between the bound particle and the unbound particle caused by the dielectrophoresis.