A method of electroporation of a biological object, comprises: introducing a carrier particle into a medium containing the biological object; applying a first electric field to the medium to induce trapping of the biological object by the carrier particle; and varying at least one parameter of the first electric field so as to induce electroporation of the biological object.

Systems, methods, and devices are described herein for identifying, monitoring, isolating, or selecting a cell having a predefined characteristic in a mixed population of cells utilizing a combination of any one or more of iDEP, a region of localized field enhancement, a variable frequency electric field, a wide bandwidth amplifier, and/or an imaging apparatus.

The present invention provides devices and methods to separate and concentrate target protein species at a microliter scale and to generate reagents to those variants with exquisite selectivity for specific protein isoforms using only picograms of target material.

A machine or apparatus featuring a first processor and a second processor. The first processor is configured to receive a mixture of fluid, valuable material and unwanted material and a functionalized polymer coated member configured to attach to the valuable material in an attachment rich environment, and provide an enriched functionalized polymer coated member having the valuable material attached thereto. The second processor is configured to receive a fluid and the enriched functionalized polymer coated member in a release rich environment to release the valuable material, and provide the valuable material released from the enriched functionalized polymer coated member.

A machine or apparatus featuring a first processor and a second processor. The first processor is configured to receive a mixture of fluid, valuable material and unwanted material and a functionalized polymer coated member configured to attach to the valuable material in an attachment rich environment, and provide an enriched functionalized polymer coated member having the valuable material attached thereto. The second processor is configured to receive a fluid and the enriched functionalized polymer coated member in a release rich environment to release the valuable material, and provide the valuable material released from the enriched functionalized polymer coated member.

In an example of the disclosure, carrier liquid is supplied to a container with a set of walls defined at least partially by a surface of an electrode. The carrier liquid is caused to move through a container via a carrier liquid flow path to sequentially encounter a set of accumulation elements. Each accumulation element is situated between adjacent walls. A voltage is applied to the electrode to generate an electric field between the electrode surface and the set of accumulation elements. The electric field causes contaminant from the carrier liquid to adhere to the set of accumulation elements.

In an example of the disclosure, carrier liquid is supplied to a container with a set of walls defined at least partially by a surface of an electrode. The carrier liquid is caused to move through a container via a carrier liquid flow path to sequentially encounter a set of accumulation elements. Each accumulation element is situated between adjacent walls. A voltage is applied to the electrode to generate an electric field between the electrode surface and the set of accumulation elements. The electric field causes contaminant from the carrier liquid to adhere to the set of accumulation elements.

An apparatus for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The electric field is applied to at least one electrode having a plurality of concentric rings or concentric arcs extending radially outwards from a center point, electrically connected to a voltage source such that when voltage is applied to the at least one electrode, the concentric rings or concentric arcs alternate in voltage potential.

A fluid entrained particle separator may include an inlet passage to direct particles entrained in a fluid, a first separation passage branching from the inlet passage, a second separation passage branching from the inlet passage and electrodes to create electric field exerting a dielectrophoretic force on the particles to direct the particles to the first separation passage or the second separation passage, wherein the first separation passage, the second separation passage, the electric field and the dielectrophoretic force extend in a plane.

An object trapping device enables efficiently trapping a plurality of objects in a specific combination. Each of a first electrode pair (13), a second electrode pair (14), and a third electrode pair (15) in an electrode pair group (3) is applied with an individual AC voltage and traps an object by dielectrophoresis generated in accordance with the AC voltage that is applied.