A method and device performing the method for estimation of cell count, such as sperm cell count, is disclosed. The device may be a kit including a cartridge configured to hold fluid, such as seminal fluid, and an instrument configured to centrifuge the cartridge. The cartridge and instrument are configured such that, during operation or centrifugation, they are securely attached to each other. The cartridge has a component with a defined cross-sectional volume. The defined cross-sectional volume is used to mark the component with markings, allowing a user of the device to read the markings and estimate cell volume and, thus, concentration. Various embodiments of the device are disclosed.

Embodiments for a portable and compact centrifugation and thermal management system capable of separating and transporting biological samples while maintaining sample quality for periods of shipment time are described. A compact, automatic centrifuge holding exactly one sample tube is inside an insulating and thermally managed container suitable for standard shipping. A rotor to retain a sample tube is pre-balanced. An electronic controller starts, times and stops centrifugation automatically, responsive to placement of a lid. Thermal management may comprise a phase change material. Embodiments are free of user controls. Embodiments are free of the need for external power or external control.

Embodiments for a portable and compact centrifugation and thermal management system capable of separating and transporting biological samples while maintaining sample quality for periods of shipment time are described. A compact, automatic centrifuge holding exactly one sample tube is inside an insulating and thermally managed container suitable for standard shipping. A rotor to retain a sample tube is pre-balanced. An electronic controller starts, times and stops centrifugation automatically, responsive to placement of a lid. Thermal management may comprise a phase change material. Embodiments are free of user controls. Embodiments are free of the need for external power or external control.

A liquid handling device having an axis of rotation about which the device can be rotated to drive liquid flow. The device includes a vented upstream chamber having an outlet port and an unvented chamber including an inlet port to receive liquid from the outlet port of the upstream chamber and an outlet port radially outward the inlet port. The device further includes a vented downstream chamber having an inlet port to receive liquid from the outlet port of the unvented chamber. A downstream conduit connects the outlet port of the unvented chamber to the inlet port of the downstream chamber and includes a bend radially inward of the outlet port of the unvented chamber. An upstream conduit connects the outlet port of the upstream chamber to the inlet port of the unvented chamber.

A liquid handling device having an axis of rotation about which the device can be rotated to drive liquid flow. The device includes a vented upstream chamber having an outlet port and an unvented chamber including an inlet port to receive liquid from the outlet port of the upstream chamber and an outlet port radially outward the inlet port. The device further includes a vented downstream chamber having an inlet port to receive liquid from the outlet port of the unvented chamber. A downstream conduit connects the outlet port of the unvented chamber to the inlet port of the downstream chamber and includes a bend radially inward of the outlet port of the unvented chamber. An upstream conduit connects the outlet port of the upstream chamber to the inlet port of the unvented chamber.

A method for operating a centrifugal separator associated with a prime mover and/or a working machine. The centrifugal separator comprises at least one rotatably mounted rotor that can be rotated at a variable speed via a drive controlled by a control unit. The method is characterized in that the speed of the rotor is controlled according to the sound emissions of the prime mover and/or according to the sound emissions of the working machine.

A method for operating a centrifugal separator associated with a prime mover and/or a working machine. The centrifugal separator comprises at least one rotatably mounted rotor that can be rotated at a variable speed via a drive controlled by a control unit. The method is characterized in that the speed of the rotor is controlled according to the sound emissions of the prime mover and/or according to the sound emissions of the working machine.

A method for clarifying a flowable starting product of solids using a self-emptying centrifuge in continuous operation in a centrifugal field in the rotating drum. A continuous draining of at least one clarified liquid phase occurs and solids emptying occurs repeatedly via outlet openings that are intermittently opened and closed. The emptying of solids from the drum of the centrifuge is initiated, the speed of the drum of the centrifuge is reduced, the solids are emptied from the solids collection chamber of the drum after lowering the speed of the drum, and the emptying of the solids from the drum is ended.

A method for clarifying a flowable starting product of solids using a self-emptying centrifuge in continuous operation in a centrifugal field in the rotating drum. A continuous draining of at least one clarified liquid phase occurs and solids emptying occurs repeatedly via outlet openings that are intermittently opened and closed. The emptying of solids from the drum of the centrifuge is initiated, the speed of the drum of the centrifuge is reduced, the solids are emptied from the solids collection chamber of the drum after lowering the speed of the drum, and the emptying of the solids from the drum is ended.

Provided herein are devices and methods of processing a sample that include, in several embodiments, rotating one or more microfluidic chips that are mounted on a support plate using a motor driven rotational chuck. By spinning one or more of the microfluidic chips about a common center of rotation in a controlled manner, high flow rates (and high shear forces) are imparted to the sample in a controlled manner. Each microfluidic chip can be rotated 180° on the support plate so that the sample can be run back-and-forth through the microfluidic devices. Because the support plate can be driven at relatively high RPMs, high flow rates are generated within the microfluidic chips. This increases the shear forces on the sample and also decreases the processing time involved as the sample can quickly pass through the shear-inducing features of the microfluidic chip(s).