A phage and use thereof in soil remediation are disclosed. The phage φYSZPK has been deposited at the China Center for Type Culture Collection on Aug. 1, 2018 under Accession No. CCTCC M 2018516, and its taxonomic designation is Pseudomonas aeruginosa and Klebsiella phage φYSZPK. Biochar and the screened phage are combined and returned into contaminated soil to synergistically control and deeply track and inactivate transmission and spread of antibiotic resistance pathogenic bacteria and resistance genes in a soil-vegetable system. The combination of the biochar and the phage φYSZPK not only clearly improves the functional stability of microbial community in the soil-vegetable system, but also significantly alleviates the dissemination of the antibiotic resistance pathogenic bacteria in the soil-vegetable system to prevent secondary pollution, thereby providing a new solution for biological remediation and control of farmland soil contaminated by antibiotic resistance pathogenic bacteria in China.

Methods to remediate a contaminated material are provided. In one embodiment, a biocatalyst that digests hydrocarbon contaminants is activated with a nutrient and the activated biocatalyst is combined with the contaminated material and water to form a mixture. The mixture is incubated for a period of time, and the level of contaminant in the mixture is determined to ascertain whether to incubate further, add additional biocatalyst mix, or provide the remediated material for further processing. In one embodiment, the remediated material is provided for reuse or recycling with a second material, such as a construction aggregate. The method is particularly suited for remediation of drill cuttings, mine tailings, hydrocarbon-contaminated soil, and the like.

Methods to remediate a contaminated material are provided. In one embodiment, a biocatalyst that digests hydrocarbon contaminants is activated with a nutrient and the activated biocatalyst is combined with the contaminated material and water to form a mixture. The mixture is incubated for a period of time, and the level of contaminant in the mixture is determined to ascertain whether to incubate further, add additional biocatalyst mix, or provide the remediated material for further processing. In one embodiment, the remediated material is provided for reuse or recycling with a second material, such as a construction aggregate. The method is particularly suited for remediation of drill cuttings, mine tailings, hydrocarbon-contaminated soil, and the like.

The present invention discloses a strain of Achromobacter xylosoxidans with monomethylamine degradability and the application thereof. This strain, named Achromobacter xylosoxidans GDUTAN5, was deposited on May 24, 2017 in the China Center for Type Culture Collection in Wuhan University (No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province) with a deposit number of CCTCC NO: M 2017285. This Achromobacter xylosoxidans GDUTAN5 was Gram-negative and rod-shaped, and the colony appeared to be round, light yellow, opaque and smooth, having a diameter of 1-2 mm. The Achromobacter xylosoxidans GDUTAN5 of the present invention can be applied to environmental restoration, degrading monomethylamine in the environment at a high degradation efficiency. When it degraded monomethylamine for 96 h at a substrate concentration of 5 mg/L, the degradation efficiency could reach 92.3%.

A methods and systems for desalination and decontamination for solid waste are disclosed, including a method comprising decontaminating a volume of contaminated, desalinated drill cuttings by removing at least a portion of contaminants associated with the contaminated, desalinated drill cuttings, the desalinated drill cuttings being drill cuttings that have been desalinated by a desalination unit.

A methods and systems for desalination and decontamination for solid waste are disclosed, including a method comprising decontaminating a volume of contaminated, desalinated drill cuttings by removing at least a portion of contaminants associated with the contaminated, desalinated drill cuttings, the desalinated drill cuttings being drill cuttings that have been desalinated by a desalination unit.

A method for biological hydrosynthesis, energy generation and storage and/or topsoil restoration comprising the steps of: undertaking a primary amendment of a site with a first catalyst and a second catalyst, wherein the first catalyst and the second catalyst are applied to at least a portion of the site such that a matrix of biological energy generation points are constructed on about 5% of the site by area; undertaking a secondary amendment of the site with the first catalyst and the second catalyst, wherein the first catalyst and the second catalyst are applied to at least a portion of the site such that a matrix of biological energy generation points are constructed on about 20% of the site by area, and undertaking a tertiary amendment of the site with the first catalyst and the second catalyst, wherein the first catalyst and the second catalyst are applied to at least a portion of the site such that a matrix of biological energy generation points are constructed on about 75% of the site by area, wherein the primary amendment, the secondary amendment and the tertiary amendment of the site are each conducted at least once.

A method for restoring a contaminated site comprising the steps of: undertaking a primary amendment of a contaminated site with a first catalyst and a second catalyst, wherein the first catalyst and the second catalyst are applied to at least a portion of the contaminated site such that a matrix of biological energy generation points are constructed on about 5% of the contaminated site by area; undertaking a secondary amendment of the contaminated site with the first catalyst and the second catalyst, wherein the first catalyst and the second catalyst are applied to at least a portion of the contaminated site such that a matrix of biological energy generation points are constructed on about 20% of the contaminated site by area, and undertaking a tertiary amendment of the contaminated site with the first catalyst and the second catalyst, wherein the first catalyst and the second catalyst are applied to at least a portion of the contaminated site such that a matrix of biological energy generation points are constructed on about 75% of the contaminated site by area, wherein the contaminated site is a contaminated site that has excessive acidity, alkalinity and/or sodic contamination, and wherein the primary amendment, the secondary amendment and the tertiary amendment of the contaminated site are each conducted at least once and wherein the outcome of those amendments is the improved capacity of the soil of the contaminated site to balance hydrogen compounds such that excessive acidity, alkalinity or sodic properties of the contaminated site are ameliorated.

Presented herein are Synechococcus strains engineered to express the bacterial ethylene-forming enzyme (EFE) that exhibit unstable ethylene production due to toxicity and genomic instability induced by accumulation of the EFE-byproduct guanidine. Co-expression of EFE and Sll1077 significantly enhanced genomic stability and enabled the resulting Synechococcus strain GD-EFE7942 to achieve sustained high-level ethylene production. The engineered strains and methods disclosed herein are useful for guanidine degradation pathways and for ethylene bioproduction in cyanobacteria.

The present invention relates to a process for the production and recovery of a bio-surfactant extract from Bacillus sp. MCC0156. Further, the present invention relates to a thermostable bio-surfactant extract consisting of 1-Pentanonacontene and 3-hydroxy-16-methylheptadecanoic acid with excellent emulsification and oil displacement activity for applications in agriculture and oil recovery.