The present invention discloses a strain of Pseudomonas aeruginosa with monomethylamine degradability and the application thereof. This strain, named Pseudomonas aeruginosa GDUTAN1, was deposited on May 24, 2017 in the China Center for Type Culture Collection in Wuhan University, Wuhan City, Hubei Province with a deposit number of CCTCC NO.: M 2017283. This Pseudomonas aeruginosa GDUTAN1 was Gram-negative and rod-like, and round, green and opaque in the colony morphology, having a diameter of 1-2 mm. The Pseudomonas aeruginosa GDUTAN1 of the present invention can be applied to environmental remediation, degrading monomethylamine in the environment at a high degradation efficiency. When it degrades monomethylamine for 96 h at a substrate concentration of 50-140 mg/L, the degradation efficiency can reach more than 99%.

A method and a system for in-situ remediation of recalcitrant organic and inorganic contaminants in an environmental medium are disclosed. Dissolved gases from water and an oil are removed to form degassed water and a degassed oil. The degassed water and the degassed oil are mixed to form a surfactant-free oil-in water emulsion. The surfactant-free oil-in-water emulsion is injected into the environmental medium, thereby producing anaerobic conditions to cause indigenous anerobic bacteria to biodegrade residual concentrations of the contaminants in the environmental medium.

A method and a system for in-situ remediation of recalcitrant organic and inorganic contaminants in an environmental medium are disclosed. Dissolved gases from water and an oil are removed to form degassed water and a degassed oil. The degassed water and the degassed oil are mixed to form a surfactant-free oil-in water emulsion. The surfactant-free oil-in-water emulsion is injected into the environmental medium, thereby producing anaerobic conditions to cause indigenous anerobic bacteria to biodegrade residual concentrations of the contaminants in the environmental medium.

Provided is a method for disposing of contaminated deposit soil and recycled reclamation soil using the same and, more specifically, a method for disposing of contaminated dredged soil, the method comprising the steps of: seeding a mixed strain NIX51 (KACC81038BP) in the contaminated dredged soil to primarily dispose of contaminated materials in a bioreactor; and washing the degraded soil, which has been primarily disposed of, with a washing solution containing at least one selected from the group consisting of citric acid, oxalate, carbonic acid (H.sub.2CO.sub.3), and nitric acid, to secondarily dispose of heavy metals.

Provided is a method for disposing of contaminated deposit soil and recycled reclamation soil using the same and, more specifically, a method for disposing of contaminated dredged soil, the method comprising the steps of: seeding a mixed strain NIX51 (KACC81038BP) in the contaminated dredged soil to primarily dispose of contaminated materials in a bioreactor; and washing the degraded soil, which has been primarily disposed of, with a washing solution containing at least one selected from the group consisting of citric acid, oxalate, carbonic acid (H.sub.2CO.sub.3), and nitric acid, to secondarily dispose of heavy metals.

A permeable reactive barrier having two or more layers of a geotextile fabric inoculated with a bioremediation microbe is provided. The permeable reactive barrier further includes two or more layers of coarse-grained geological material separating the two or more layers of geotextile fabric such that any pair of adjacent layers of geotextile fabric is separated by a layer of coarse-grained geological material. The permeable reactive barrier includes a perforated metal casing surrounding and containing the layers of coarse-grained geological materials and geotextile fabric.

A permeable reactive barrier having two or more layers of a geotextile fabric inoculated with a bioremediation microbe is provided. The permeable reactive barrier further includes two or more layers of coarse-grained geological material separating the two or more layers of geotextile fabric such that any pair of adjacent layers of geotextile fabric is separated by a layer of coarse-grained geological material. The permeable reactive barrier includes a perforated metal casing surrounding and containing the layers of coarse-grained geological materials and geotextile fabric.

The present invention provides for obtaining microemulsion formulations without the presence of an oil phase for the removal of oil from solid surfaces, specifically in sandstone rocks. Oil impregnation on solid surfaces is a major challenge due to the need to purify environments affected by environmental disasters and accidents related to the oil and gas industry. Thus, the study and development of technologies capable of preventing or remedying the effects that oil contamination on solid surfaces can cause is justified, Several technologies present themselves as promising candidates for the remediation of contaminated surfaces, but many of them can have high execution costs, as in the case of classic microemulsions, or generation of secondary residues. The process of the present invention does not present these disadvantages, consisting of obtaining microemulsion systems containing nonionic surfactants of different degrees of ethoxylation (lauryl alcohol with 10, 8 and 6 ethoxylation bonds), short-chain alcohol (1-butanol) and distilled water for the extraction of heavy crude oil impregnated in sandstone rocks. The formulations presented are capable of using low active matter compositions, unlike classical microemulsions which, in most cases, require a high amount of surfactant.

The present invention provides for obtaining microemulsion formulations without the presence of an oil phase for the removal of oil from solid surfaces, specifically in sandstone rocks. Oil impregnation on solid surfaces is a major challenge due to the need to purify environments affected by environmental disasters and accidents related to the oil and gas industry. Thus, the study and development of technologies capable of preventing or remedying the effects that oil contamination on solid surfaces can cause is justified, Several technologies present themselves as promising candidates for the remediation of contaminated surfaces, but many of them can have high execution costs, as in the case of classic microemulsions, or generation of secondary residues. The process of the present invention does not present these disadvantages, consisting of obtaining microemulsion systems containing nonionic surfactants of different degrees of ethoxylation (lauryl alcohol with 10, 8 and 6 ethoxylation bonds), short-chain alcohol (1-butanol) and distilled water for the extraction of heavy crude oil impregnated in sandstone rocks. The formulations presented are capable of using low active matter compositions, unlike classical microemulsions which, in most cases, require a high amount of surfactant.

The present application is related to a method for controlling heavy metal scouring pollution in mines by using a humified product, which comprises steps of: (1) carrying out mixed fermentation on sludge and hyperthermophiles to breed polar humified groups, and then carrying out mixed fermentation on the fermented material and a native material of humus precursors to induce massive production of humus until the humus content reaches a required standard; and (2) mixing and maintaining the humified product of the sludge reaching the standard with mine soil polluted by heavy metals to remedy the polluted mine.