Systems, instruments, methods, and compositions are described involving removing a portion of the epidermis within a donor site on a subject, and harvesting dermal plugs within the donor site. An injectable filler is formed by mincing the dermal plugs. The injectable filler is configured for injecting into a recipient site on the subject.

Described herein is conduit material that causes minimal inflammatory reaction, and serves as a structural guide for regenerating nerve tissue (e.g., axons). Thus, the invention is directed to methods of treating a nerve injury in an individual in need thereof. The methods employ an isolated, naturally occurring epineural sheath, and can be used, for example, to regenerate nerve tissue in an individual in need thereof. Also provided herein is a device for harvesting an epineural sheath.

Various implementations include a cutting tool for the horizontal incision of tendons. The tool comprises a stem extending along a longitudinal axis, having a distal end, a proximal end and a cutting head, located close to said distal end, having a base plate, having a longitudinal extension axis parallel to the longitudinal axis of the stem, and a blade extending on a plane parallel to the base plate. The blade has a plurality of cutting edges inclined with respect to the longitudinal axis of the cutting head according to different angles, so as to identify at least two vertices defined by two V-shaped profiles.

Various implementations include a tool for the subcutaneous cutting of tendons. The tool includes a shaft, arranged along a longitudinal axis, having a proximal end and a distal end, a grip, provided at the proximal end, comprising a fixed handle and a movable trigger, and a cutting head placed at the distal end, which comprises a tendon harvesting tip provided with a groove and a blade. The blade and the harvesting tip are movable relative to each other on a plane which contains the longitudinal axis of the shaft. The blade is inclined with respect to the longitudinal axis of said shaft.

The present disclosure relates to a tissue collection apparatus. The tissue collection apparatus comprises a housing defining an inlet and an outlet, a first filter disposed within the housing, a second filter disposed within the housing, the second filter configured to isolate tissue particles of a desired size that pass through the first filter under the application of an aspiration force applied through the housing. A method of harvesting tissue is also disclosed.

A graft collector designed to facilitate and offer greater precision in performing the cutting and “dissection” of an area of the donor tissue in grafting operations. The graft collector is set as an anatomical grip handle based on a support head and fixation of a removable curved or angled blade. The cutting blade is provided with a cutting edge on two opposite sides, being folded in two points at 90°. The constructive arrangement of the graft collector allows the fixation of the cutting blade and its loosening and release for replacement. The said graft collector is a precise and anatomical grip instrument, designed for the good manual performance of the professional when cutting and removing the tissue to be used as a graft.

A method for cell printing is disclosed. The method includes generating a receiver substrate, ablating or removing a portion of the receiver substrate via a first laser to expose a target layer, generating a donor substrate containing a back surface and a front surface, applying a coating of donor material to the front surface. The method further includes aligning the front surface of the donor substrate to be parallel to and facing the receiver substrate, wherein the donor material is disposed adjacent to the target layer, and irradiating the coating through the back surface of the donor substrate with one or more laser pulses produced by a second laser to transfer a portion of the donor material to the target layer. A system for cell printing is also disclosed.

A dissecting device for dissecting tissue surrounding a blood vessel in a living body. The dissecting device includes a grasping section that includes an insertion lumen. The dissecting device includes a dissecting member at the distal portion of the grasping section. The dissecting member is insertable into the living body along the blood vessel to dissect the tissue surrounding the blood vessel in the living body. The dissecting member includes a treating section and a protruding section. The treating section performs a predetermined treatment of a branch vessel branching from the blood vessel. The protruding section protrudes from the treating section in the thickness direction of the treating section. The protruding section includes a guide section configured to guide the branch vessel toward the treating section.

In one aspect, the present disclosure relates to a device for obtaining biological tissue. In some embodiments, the device can include a first row of a plurality of hollow tubes; a second row of a plurality of hollow tubes, adjacent the first row of hollow tubes; a third row of a plurality of hollow tubes, adjacent the second row of hollow tubes, the first, second and third rows forming an array of hollow tubes; wherein each hollow tube can include at least one point at the distal end of the hollow tube, the plurality of rows forming a two dimensional array of hollow tubes; wherein an inner diameter of the at least one tube is less than about 1 mm, the distal end of each of the hollow tubes is configured to be inserted into a biological tissue donor site to remove a portion of tissue therefrom when each of the hollow tubes is withdrawn from the donor site.

A cutting tool for the vertical incision of tendons comprising a shaft extending along a longitudinal axis, having a distal end and a proximal end, and a cutting head, located close to said distal end, having a plurality of blades. The cutting head lies on a plane parallel to the longitudinal axis of the shaft and is connected to the latter by a coupling inclined with respect to the longitudinal axis by an angle from 10° to 20°, preferably of 15°.