Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.

A vaporization device is provided having a plurality of pens each having a battery, a mouthpiece, and a ventilated end. A vaporization device is also provided which is disposable having a plurality of pens, each having a vaporization chamber containing a coil, a battery, a PCB, a sensor, and a tank containing e-liquid for vaporization in the pen vaporization chamber through the coil of the pen to create a vaporization product which is mixed in a vapor port of the device through which a plurality of vaporization products are delivered to a user through the mouthpiece of the device in a single vaporization event.

A bottle for holding liquids that is biodegradable and that is made from environmentally sustainable materials. The bottle includes a body, a shoulder, a neck portion, and a cap. According to one aspect, the neck portion of the bottle is made of bioplastic, and the shoulder and body of the bottle are made from thermoformed pulp. The bioplastic neck can mate with, and be adhered to, the shoulder, and the shoulder can mate with, and be adhered to, the pulp body.

A degradable containment includes an outer structural layer constructed of a pulp material, a central carrier layer constructed of a biopolymer, and an interior nanomaterial layer constructed of a nanomaterial.

A glass neck for attachment to a container of a material other than glass is described. The glass neck comprises a wall defining a passageway having a first end and a second end, container-attachment means being provided at the first end and the second end defining a glass rim.

Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.

A bottle for holding liquids that is biodegradable and that is made from environmentally sustainable materials. The bottle includes a body, a shoulder, a neck portion, and a cap. According to one aspect, the neck portion of the bottle is made of bioplastic, and the shoulder and body of the bottle are made from thermoformed pulp. The bioplastic neck can mate with, and be adhered to, the shoulder, and the shoulder can mate with, and be adhered to, the pulp body.

Molded pulp containers with fused junctions and methods of making of same are provided. The present invention provides a pulp container, comprising a molded pulp body, wherein the body comprising a bottom and at least one side wall forming a cavity, wherein the at least one side wall comprising a upper rim, a neck, wherein the neck comprising a upper closure part and a lower rim, a fused junction, wherein a part of the upper rim of the at least one sidewall of the body and a part of the lower rim of the neck are aligned and fused together. The fused junction may be formed by ultrasonic welding.

Collapsible indoor and outdoor containers having a collapsible wall section with shape-retaining characteristics are disclosed. In particular, collapsible coolers, fish bins, and wheeled vertical bins are provided. The collapsible wall section may include foldable tiers of a flexible material, each tier having at least one stable, relatively expanded position and at least one stable, relatively collapsed position; and an intervening, non-folding tier composed of a different, relatively stiff or rigid material. The coolers, fish bins, and wheeled vertical bins are each advantageously provided with a collapsible insert container that nests within and collapses together with a shell of the respective container, the respective wall folds of the shell and insert meshing together to result in a combined collapsed vertical dimension as small or nearly as small as that of the collapsed shell alone.