Disclosed are instruments and methods for acquiring co-registered orthogonal fluorescence and photoacoustic volumetric projections of an interrogated object. In an embodiment, an instrument includes an imaging tank filled with a liquid coupling medium. An object positioning mechanism is configured to position the interrogated object inside the coupling medium and to rotate the interrogated object. An optical excitation unit that is fixed with respect to the tank is configured to induce both fluorescence and photoacoustic responses inside the interrogated object using the same optical excitation spectrum and the same irradiation pattern at the surface of the interrogated object. An array of unfocused photoacoustic transducers is fixed with respect to the tank, and each element of the array is configured to detect photoacoustic signals generated inside the interrogated object. The array is arranged such that a plane connecting the rotation axis and a central portion of the array cuts through the interrogated object separating the two sides irradiated by the optical excitation unit. An optical detector that is fixed with respect to the tank is configured to register planar projections of sources of fluorescence generated inside the object. A data acquisition unit is configured to synchronize acquisition of photoacoustic data, acquisition of fluorescence data, optical excitation, and rotational position of the interrogated object.

Various systems, devices, NO.sub.2 absorbents, NO.sub.2 scavengers and NO.sub.2 recuperator for generating nitric oxide are disclosed herein. According to one embodiment, an apparatus for converting nitrogen dioxide to nitric oxide can include a receptacle including an inlet, an outlet, a surface-active material coated with an aqueous solution of ascorbic acid and an absorbent wherein the inlet is configured to receive a gas flow and fluidly communicate the gas flow to the outlet through the surface-active material and the absorbent such that nitrogen dioxide in the gas flow is converted to nitric oxide.

Various systems, devices, NO.sub.2 absorbents, NO.sub.2 scavengers and NO.sub.2 recuperator for generating nitric oxide are disclosed herein. According to one embodiment, an apparatus for converting nitrogen dioxide to nitric oxide can include a receptacle including an inlet, an outlet, a surface-active material coated with an aqueous solution of ascorbic acid and an absorbent wherein the inlet is configured to receive a gas flow and fluidly communicate the gas flow to the outlet through the surface-active material and the absorbent such that nitrogen dioxide in the gas flow is converted to nitric oxide.

It includes the steps of (1) preparing step; (2) anesthetizing step; (3) electrodes installing step; and (4) focal onset seizures inducing step. First, a live animal is anesthetized. The electrodes are installed on a working zone of its brain. A first chemical liquid is injected beneath the cerebral cortex of this animal. At two different times, a first electric stimulation and a second electric stimulation are activated by the controller respectively so as to generate a first and a second focal onset seizure accordingly. Thus, it can generate focal onset seizures. It is easy to change the electric stimulation position, numbers and type. In addition, the survival rate of a larger-sized animal after anesthesia is higher.

It includes the steps of (1) preparing step; (2) anesthetizing step; (3) electrodes installing step; and (4) focal onset seizures inducing step. First, a live animal is anesthetized. The electrodes are installed on a working zone of its brain. A first chemical liquid is injected beneath the cerebral cortex of this animal. At two different times, a first electric stimulation and a second electric stimulation are activated by the controller respectively so as to generate a first and a second focal onset seizure accordingly. Thus, it can generate focal onset seizures. It is easy to change the electric stimulation position, numbers and type. In addition, the survival rate of a larger-sized animal after anesthesia is higher.

A system for preventing animal suffocation because of a bag is disclosed. The system includes an attachment for the bag having a first panel and a second panel. A hinge element attaches the first panel with the second panel. The first panel is configured to attach to a first outward facing portion of the bag and the second panel and is configured to attach to a second outward facing portion of the bag. A one-way air flow valve is integral with at least one of the first panel and the second panel and is configured to allow air to flow from an outside environment into the bag only when an inward force acts on the valve and pulls air from outside the bag inward inside the bag.

An imaging bed for use with test subjects that keeps the subject sedated and warm throughout the imaging process. In embodiments, the bed uses radiant heating or cooling by pushing a heated or chilled fluid through the bed to control the temperature of the test subject during the imaging process. The imaging bed can also incorporate an integrated anesthesia channel. In embodiments, an exhaust channel removes the cool air, as well as the unused anesthesia from the imaging bed though an exhaust port. Various embodiments include a docking mechanism for easy connection of anesthesia to the bed, as well as an adapter for fitting smaller or multiple subjects simultaneously within the imaging bed.

An imaging bed for use with test subjects that keeps the subject sedated and warm throughout the imaging process. In embodiments, the bed uses radiant heating or cooling by pushing a heated or chilled fluid through the bed to control the temperature of the test subject during the imaging process. The imaging bed can also incorporate an integrated anesthesia channel. In embodiments, an exhaust channel removes the cool air, as well as the unused anesthesia from the imaging bed though an exhaust port. Various embodiments include a docking mechanism for easy connection of anesthesia to the bed, as well as an adapter for fitting smaller or multiple subjects simultaneously within the imaging bed.

A versatile subject bed for use with one or more test subjects that is configured to keep the subjects sedated throughout either a surgical procedure or imaging process. In embodiments, the subject bed can include interchangeable manifolds allowing the platform to hold up to four subjects at the same time. The interchangeable manifolds can include configurations designed to provide for a single subject, two subjects or four subjects. The four-subject manifold can include an elevated platform that holds two subject side by side and allows the subjects to be stacked during imaging or other procedures. The subject bed can also incorporate an integrated anesthesia channel. Various embodiments include a docking mechanism for easy connection of anesthesia to the platform, as well as an adapter for fitting smaller or multiple subjects simultaneously within the subject bed.

A versatile subject bed for use with one or more test subjects that is configured to keep the subjects sedated throughout either a surgical procedure or imaging process. In embodiments, the subject bed can include interchangeable manifolds allowing the platform to hold up to four subjects at the same time. The interchangeable manifolds can include configurations designed to provide for a single subject, two subjects or four subjects. The four-subject manifold can include an elevated platform that holds two subject side by side and allows the subjects to be stacked during imaging or other procedures. The subject bed can also incorporate an integrated anesthesia channel. Various embodiments include a docking mechanism for easy connection of anesthesia to the platform, as well as an adapter for fitting smaller or multiple subjects simultaneously within the subject bed.