The present disclosure provides, inter alia, compositions, methods, and kits for improving the success rate of in vitro fertilization in small ruminants using microbiome profiles. According to some aspects, the present disclosure provides a method and kit for pairing female donor subjects with female recipient subjects, and for selecting female donor subjects. According to other aspects, the present disclosure provides a method of generating a microbiome database.

Technologies for cryopreserving ungulate embryos for implantation into recipient females are described.

Technologies for cryopreserving ungulate embryos for implantation into recipient females are described.

Technologies for cryopreserving ungulate embryos for implantation into recipient females are described.

Technologies for cryopreserving ungulate embryos for implantation into recipient females are described.

Methods and compositions directed to altering a population of sRNAs in a sperm using vesicles isolated from an epididymosome are provided. Methods and compositions directed to altering a population of sRNAs in an oocyte using vesicles isolated from an epididymosome are also provided. Methods for altering an sRNA population in a sperm or an oocyte can be used to prevent, or reduce the severity of, a disease, disorder, or condition that would otherwise be inherited by progeny. For example, certain epigenetic inherited conditions due to paternal effects, such as certain metabolic and stress disorders and conditions, can be ameliorated in progeny using sperm or oocytes having an altered sRNA population.

Methods and compositions directed to altering a population of sRNAs in a sperm using vesicles isolated from an epididymosome are provided. Methods and compositions directed to altering a population of sRNAs in an oocyte using vesicles isolated from an epididymosome are also provided. Methods for altering an sRNA population in a sperm or an oocyte can be used to prevent, or reduce the severity of, a disease, disorder, or condition that would otherwise be inherited by progeny. For example, certain epigenetic inherited conditions due to paternal effects, such as certain metabolic and stress disorders and conditions, can be ameliorated in progeny using sperm or oocytes having an altered sRNA population.

Methods for improving the functionality and/or fertility of sperm, for example, by enhancing motility and/or extending the lifespan of sperm by subjecting the isolated sperm to a starvation protocol and/or ionophore are provided. Such methods may be used in, for example, artificial insemination to reduce the number of sperm needed for insemination and to improve conception rates.

Methods for improving the functionality and/or fertility of sperm, for example, by enhancing motility and/or extending the lifespan of sperm by subjecting the isolated sperm to a starvation protocol and/or ionophore are provided. Such methods may be used in, for example, artificial insemination to reduce the number of sperm needed for insemination and to improve conception rates.

The present invention relates to dispensing instruments and methods for introducing treatment material and fluid-like material with an embryo into a uterus. More specifically, the present invention relates to apparatuses for delivering a fertilized egg or embryo into a maternal uterine endometrium in humans or any other mammalian species and associated computer programs for controlling said apparatuses.