Methods of recovering cannabinoids from cell cultures include methods comprising steps of separating the cell culture at a temperature above the melting point of the cannabinoid to separate a light phase comprising liquid state cannabinoid from a heavy phase; and methods comprising treating the cell culture at a temperature below the melting point of the cannabinoid to separate a light phase from a heavy phase comprising solid state cannabinoid. Other methods include contacting the culture with a water-miscible solvent to form a water-miscible phase and an aqueous phase, separating the two phases and recovering the cannabinoid. Other methods include contacting the culture with a water-immiscible solvent to form a water-immiscible phase and an aqueous phase, separating the two phases, and recovering the cannabinoid. Other methods include washing the inner surface of a fermentation vessel with alkaline solution to recover cannabinoid attached to the vessel surface. Various methods make use of aqueous solvent systems comprising no organic solvent, aqueous solvent systems comprising added water-miscible organic solvent, and dual-phase aqueous/water-immiscible solvent systems.

The present invention relates to the field of perfumery, that is, the art or process of making a fragrant substance be it natural or synthetic.

The present invention discloses a method of obtaining a better reconstituted absolute NNA+ (Near Natural Absolute) comprising the steps of: a) mixing a synthetic absolute or raw reconstituted absolute with an alcohol; b) freezing the solution of step (a); c) filtering the solution of step (b) through a propylene cloth to separate out non-alcohol soluble substance and to obtain a crystal clear percolate; d) transferring the percolate obtained from step (c) to an alcohol extraction unit, where said solution is boiled in a glass vessel at high temperature and vacuum to separate the alcohol; e) allowing the solution of step (d) to stand for maturation of the reconstituted absolute NNA+.

The present disclosure discloses a 1-carboxy-2-hydroxy-3-iminopropane and an extraction method thereof, and belongs to the technical fields of food, health food and medicine. A method for extracting the 1-carboxy-2-hydroxy-3-iminopropane of the present disclosure includes the following steps: (1) adding a diaphragma juglandis fructus powder into an ethanol solution for extraction and filtration to obtain a supernatant, and conducting concentration on the supernatant under reduced pressure to obtain a walnut alcohol extract; (2) adding a filter residue of step (1) into water for extraction and filtration to obtain a supernatant, and conducting concentration on the supernatant under reduced pressure to obtain a walnut water extract; and (3) mixing the walnut alcohol extract and the water extract to obtain a diaphragma juglandis fructus mixed extract, sequentially conducting elution through an AB-8 type macroporous resin column, an MCI column and an ODS column, and conducting separation to obtain a precipitate, namely the 1-carboxy-2-hydroxy-3-iminopropane. In the present disclosure, the 1-carboxy-2-hydroxy-3-iminopropane in diaphragma juglandis fructus is found for the first time and has great sedation and hypnosis activity.

This invention relates to the extraction of psychoactive compounds from organisms for use in medicine. Extraction is carried out with a strong acid or strong base to either promote or inhibit dephosphorylation. The extract in the slurry form is standardized with added excipient so that when it is dried the powdered composition has a specified total psychoactive alkaloid concentration, with a known ratio of phosphorylated to dephosphorylated psychoactive alkaloids.

A method for extracting bioactive compounds from plant materials using supercritical glycerin and at least one inert co-solvent that lowers the effective supercritical point of the supercritical glycerin to its boiling point, and the extracts obtained from the method.

The present discloses a method and system for extracting and recovering selective bioactive components from Mitragyna speciosa plant biomass with carbon dioxide comprising one or more solutes in supercritical and/or subcritical state. The method and system disclosed herein comprise the steps; (i) contacting said Mitragyna speciosa plant biomass with carbon dioxide comprising one or more solutes in supercritical and/or subcritical state. (ii) further separating solution comprising said carbon dioxide, said solute(s) and said bioactive components from said plant biomass, (iii) further transitioning said solution comprising said carbon dioxide, said solute(s)s, and said bioactive components to solid phase, (iv) further removal of said carbon dioxide, and/or said solute(s) via sublimation and recovering said selective fraction of bioactive components consisting essentially of mitragyinien, paynanthine, speciogynine, and/or 7-Hydroxymitragynine.

This disclosure describes systems, methods, and devices for phytochemical extraction. One example extraction system includes two solvent columns, a material column, and a dewaxing column. The solvent columns store and provide solvent for stripping target chemicals from plant material in the material column. The solvent mixed with target chemicals passes into the dewaxing column, where the target chemicals are separated from waxes and lipids. Cooling is applied to elements of the system by way of an open-loop CO2 refrigeration method. Solvent is moved from the solvent columns to the material column by creating a pressure differential between the two solvent columns.

A method of controlled decarboxylation of active compounds at various stages of biomass extraction is provided. The method involves partially or completely decarboxylating the biomass during at least two stages of biomass extractions. The stages may include, for example, decarboxylating prior to contact with the solvent, decarboxylating the biomass during the extraction process while it is in the slurry with solvent in the extractor, or decarboxylating after extraction.

A method for purification and extraction of cannabinoids includes: providing a cannabis oil including phospholipids and cannabinoid acids; contacting the cannabis oil with a degumming solvent, wherein the degumming solvent and cannabis oil are substantially immiscible; and separating an aqueous phase including the degumming solvent and at least a portion of the phospholipids from an oil phase including the cannabis oil. The method may further include contacting the oil phase with an extraction solvent, where the extraction solvent and oil phase are substantially immiscible; and separating an aqueous phase including the extraction solvent and at least a portion of the cannabinoid acids from a second oil solvent phase including the oil phase and/or simply the liberated cannabinoids following acidification of the extraction solvent.

This invention relates to the extraction of psychoactive compounds from organisms for use in medicine. Extraction is carried out with a strong acid or strong base to either promote or inhibit dephosphorylation. The extract in the slurry form is standardized with added excipient so that when it is dried the powdered composition has a specified total psychoactive alkaloid concentration, with a known ratio of phosphorylated to dephosphorylated psychoactive alkaloids.