A protein purification process with virus inactivation or removal uses caprylic acid (octanoic acid) at acidic pH. The method comprises caprylic acid treatment as part of the chromatographic step so as to perform viral inactivation without a discontinuous process and without the requirement of attention by personnel, particularly when the pH adjustment of the eluate is performed automatically by eluting into buffer. The method of the invention further results in mycoplasmas being inactivated, and reduced impurities like Host Cell Protein (HCP).

A method for separating substantially pure rare earth metals and other metals from a mixed source, including putting a plurality of rare earth metals and other metals into solution to define a solution containing a plurality of respective metal ions, in at least one chromatographic column, selectively capturing ions of each respective metal with a respective ligand to define a plurality of respective discrete bands, and respectively eluting captured ions of respective metal from each respective band of the at least one chromatographic column to yield a plurality of purified solutions, each respective purified solution having a high concentration of a respective metal. The bands may either be stationary with respect to the columns, or may move through the columns.

A method for purifying a mixture in a multicolumn chromatography system. The method successively and cyclically collects a raffinate, injects the mixture to be separated, collects an extract, and injects eluent. The mixture to be separated contains fructose and has a dry matter mass concentration of 45 to 55%. The method is carried out at a temperature of 50 to 62° C.

The present disclosure provides methods for determining adsorption isotherms for complex mixtures. In at least one embodiment, a method for obtaining adsorption isotherms for liquid mixtures includes providing a column comprising an adsorbent. The method includes delivering a composition to the column, the composition comprising a multi-component feed and a solvent. The method includes collecting a sample from the column and introducing the sample to a two dimensional gas chromatograph to determine a time-series concentration of one or more components of the sample. The method includes integrating the time-series concentration of at least one of the one or more components to determine an isotherm of the at least one component. The method includes obtaining quantitative information of the at least one component, based on the isotherm of the at least one component.

A method of recovering a sugar by separating a fermentation inhibitor and the sugar from a sugar solution containing the fermentation inhibitor, the method including: bringing the sugar solution containing the fermentation inhibitor into contact with a basic anion exchange resin filled into a column, followed by separation of the fermentation inhibitor and the sugar by a difference in retention time therebetween through use of water as an eluent, and separate recovery of a fraction containing the fermentation inhibitor and a fraction containing the sugar, wherein the basic anion exchange resin is previously treated with a solution containing the fermentation inhibitor.

Provided herein are methods of separating host cell lipases from an anti-LAG3 antibody or antigen binding fragment in chromatographic processes and methods of improving polysorbate-80 stability in an anti-LAG3 antibody formulation by separating host cell lipases from the anti-LAG3 antibody or antigen binding fragment using chromatographic processes. Also provided are pharmaceutical compositions comprising an anti-LAG3 antibody or antigen binding fragment and less than 2 ppm of a host cell lipase.

The present disclosure relates to a method of synthesizing cyclosporine derivatives. The method includes: providing a precursor fluid of the cyclosporine derivative, an alkaline fluid and a ClCH.sub.2OCOCl solution; premixing the precursor fluid and the alkaline fluid to obtain a premixed solution; feeding the premixed solution into a first reaction chamber, reacting to prepare a first reaction liquid; feeding the first reaction liquid into a second reaction chamber, reacting the first reaction liquid with a CO.sub.2 fluid to prepare a second reaction liquid; and reacting the second reaction liquid with the ClCH.sub.2OCOCl solution.

A new column-based purification system and approach are described for rapid separation and purification of the alpha-emitting therapeutic radioisotope .sup.211At from dissolved cyclotron targets that provide highly reproducible product results with excellent .sup.211At species distributions and high antibody labeling yields compared with prior art manual extraction results of the prior art that can be expected to enable enhanced production of purified .sup.211At isotope products suitable for therapeutic medical applications such as treatment of cancer in human patients.

There is provided a method of operating a liquid chromatography arrangement, the liquid chromatography arrangement comprising: a solvent pump arranged to flow a liquid solvent over a liquid chromatography column; a restrictor arranged to restrict the liquid solvent from leaving the liquid chromatography column; and a liquid pump arranged to provide liquid flow between the liquid chromatography column and the restrictor, the method comprising: flowing the liquid solvent through the liquid chromatography column using the solvent pump; and controlling a liquid pressure within the liquid chromatography column by providing a liquid flow between the liquid chromatography column and the restrictor using the liquid pump.

A method for removing coloration from a drug substance solution of protein preparation, in particular, antibody preparation, a method for preparing drug substance solution of protein preparations including it, as a part thereof, and highly concentrated, colorless drug substance solutions thereof are disclosed. The method removes terminal glycation products causing the coloration in the drug substance solution of protein preparation, in particular, antibody preparation, by anion-exchange chromatography, making it possible to provide colorless drug substance solutions.