An object of the present invention is to provide a method for purifying efficiently and easily a .sup.226Ra-containing solution obtained when .sup.225Ac is produced from a .sup.226Ra target, a method for producing a .sup.226Ra target by using the purified .sup.226Ra-containing solution obtained by the above purification method, and a method for producing .sup.225Ac including these above methods. The method for purifying a .sup.226Ra-containing solution according to the present invention is characterized by including an adsorption step (R1) of allowing .sup.226Ra ions to adsorb onto a carrier having a function of selectively adsorbing divalent cations by bringing a .sup.226Ra-containing solution (a) into contact with the carrier under an alkaline condition; and an elution step (R2) of eluting the .sup.226Ra ions from the carrier under an acidic condition.

A method for obtaining a liquid from a porous solid phase is described. The method comprises forming a liquid seal at a first end of a porous solid phase to which a liquid is bound, wherein liquid of the liquid seal is immiscible with the liquid bound to the solid phase, and applying a pressure differential across the porous solid phase to cause the immiscible liquid to move through the porous solid phase towards a second end of the porous solid phase, thereby displacing the liquid bound to the porous solid phase towards the second end and releasing this liquid from the second end. Recovery of liquid from the solid phase using such methods is increased compared with corresponding methods in which no liquid seal is formed. In preferred embodiments, the liquid used to form the liquid seal is a mineral oil. The methods have particular application in nucleic acid extractions which utilize capture of nucleic acid to a solid phase. Kits and apparatus for performing the methods are also described.

In a preparative separation-purification system for passing a solution containing a target component through a trap column to capture the target component in the column, and for subsequently passing an eluting solvent through the column to elute the captured component and collect it in a container, a dilution passage is merged with a collection passage for sending an eluate from the outlet end of the trap column to the collection container, and a diluting liquid is intermittently introduced through the dilution passage into the collection passage. The diluting liquid lowers the concentration of the target component in the eluate and impedes the deposition of the target component. Thus, clogging of the passage due to the deposition of the target component eluted from the trap column is effectively prevented.

A process for purification of antibody or fusion protein by affinity chromatography wherein the elution is performed with high salt concentration which reduce turbidity in protein mixture during neutralization steps. The present invention provides an improved process of purifying antibodies through affinity chromatography using high salt-based elution.

A liquid chromatography monitoring system comprises a computer or electronic controller comprising computer-readable instructions operable to: (a) draw a fluid into a syringe pump; (b) configure a valve so as to fluidically couple the pump to either a fluidic pathway through a fluidic system or to a plug that prevents fluid flow; (c) cause the syringe pump to progressively compress the fluid therein or expel the fluid to the fluidic pathway, while measuring a pressure of the fluid; (d) determine a profile of the variation of the measured pressure; (e) compare the determined profile to an expected profile that depends upon the fluid; and (f) provide a notification of a sub-optimal operating condition or malfunction if the determined profile varies from the expected profile by greater than a predetermined tolerance.

Disclosed herein is a novel method to fabricate magnetic silica nanomembranes using thin polymer cores based on silica deposition and self-wrinkling induced by thermal shrinkage. These micro- and nano-scale structures have vastly enlarged the specific area of silica, thus the magnetic silica nanomembranes can be used for solid phase extraction of nucleic acids. The magnetic silica nanomembranes are suitable for nucleic acid purification and isolation and demonstrated better performance than commercial particles in terms of nucleic acid recovery yield and integrity. In addition, the magnetic silica nanomembranes may have high nucleic acid capacity due to significantly enlarged specific surface area of silica. Methods of use and devices comprising the magnetic silica nanomembranes are also provided herein.

An apparatus for chemical separations includes a first substantially rigid microfluidic substrate defining a first fluidic port; a second substantially rigid microfluidic substrate defining a second fluidic port; and a coupler disposed between the first and second substrates, the coupler defining a fluidic path in fluidic alignment with the ports of the first and second substrates. The coupler includes a material that is deformable relative to a material of the first substrate and a material of the second substrate. The substrates are clamped together to compress the coupler between the substrates and form a fluid-tight seal.

The invention relates to a method for purifying a first fatty acid, in particular a first polyunsaturated fatty acid, using an initial mixture further comprising at least one second fatty acid and a third fatty acid, with the method comprising at least: a first step of chromatographic separation in liquid phase, using the initial mixture, making it possible to recover on the one hand a first flow enriched with a first fatty acid and on the other hand a flow enriched with a second fatty acid; a second step of chromatographic separation in liquid phase, using the first flow enriched with a first fatty acid, making it possible to recover on the one hand a second flow enriched with a first fatty acid and on the other hand a flow enriched with a third fatty acid, with the second step of chromatographic separation being carried out in a static bed chromatographic separation unit.

Disclosed is a chromatographic method for separating and purifying a recombinant human fibronectin from a genetically engineered rice seed that expresses the human fibronectin. In the method, the genetically engineered rice seed is milled, mixed with an extraction buffer, and then filtered to obtain a crude extract comprising the recombinant human fibronectin; the crude extract comprising the recombinant human fibronectin is subjected to cation exchange chromatography, so as to perform primary separation and purification, thereby obtaining a primary product comprising the recombinant human fibronectin; and the primary product is subjected to anion exchange chromatography so as to perform final separation and purification to obtain the recombinant human fibronectin as a target substance. The method is low cost and easily utilized on an industrial scale. The obtained OsrhFn target substance has a SEC-HPLC purity greater than 95% with excellent bioactivity.

A method of recovering lithium from an aqueous source is described. Lithium is extracted from the aqueous source using a sorption/desorption process to form a lithium extract. Impurities are removed from the lithium extract to form a purified lithium extract, and the purified lithium extract is concentrated using a water removal process to form a lithium concentrate. The lithium concentrate is then converted to one or more of lithium carbonate and lithium hydroxide to form a converted stream. Various streams, including some lithium-containing streams, are recycled to the sorption/desorption process.