The present invention provides an automated system and method to isolate nucleated blood cells from whole blood or bone marrow. A disc mounted to a centrifuge system with spinning rotor is used to manipulate cells by channeling fluids while subjected to high gravitational field. The disc embodies at least two axisymmetric processing stations connected by a circular channel. Each station contains multiple chambers connected by fluidic channels to controllably transfer fluids. First stage separation allows for the isolation of the buffy coat layer while the second stage separation utilizes gradient density fluids to isolate the targeted nucleated cells from the buffy coat layer in the spinning disc.

A single stage clarifier mixing assembly includes a housing having a circular sidewall and a center axis, a mixing section in the housing, a clarifier section overlying and axially aligned with the mixing section along the center axis within the housing, an inlet delivering an inlet stream to the mixing section and an agitator. The agitator is adapted for mixing the inlet stream in the mixing chamber.

An oil and grit separator system having twin vortex which uses gravity providing a twin vortex effect to remove sediment and oil from contaminated water. The system is designed to control storm water runoff and reduce the annual suspended solids loading of the granulometric particles. The conception and configuration of the system has a double action vortex. It ensures an optimal treatment level in either light or heavy rain and avoids re-suspension of particles. It also removes, separates and stores floating oil without any possible re-suspension of the matter.

A separator for separating wellbore emulsions into liquid and gaseous components has helical emulsion preheat coils encircling a single-cylinder, dual chamber firetube disposed inside a horizontal separator vessel. In use, emulsion enters the preheat coils before entering the separator vessel. The flow of emulsion through the helical coils promotes initial separation of the emulsion by means of heat transfer and centrifugal flow. Resultant centripetal force separates lighter gaseous and liquid particles toward the inside of the helical coils, while heavier emulsion fractions condense toward the outside of the helical coils. The use of helical preheat coils and a single-cylinder, dual-chamber firetube eliminate or minimize abrupt changes in emulsion flow direction that are characteristic of prior art separators, resulting in reduced wear in both the coils and the firetube.

An apparatus (1) and method for treating contaminated waste water (2). The apparatus comprises a heater (30), a feed apparatus (11, 12, 13, 14), for supplying contaminated waste water (2) to the heater (30) and a centrifuge (50, 60) downstream of the heater (30). A heat exchanger (20) is also provided which has a first channel (22) and a second channel (24). The first channel (22) is connected in a flow path extending between the feed apparatus (11, 12, 13, 14) and the centrifuge (50, 60); and a waste water outlet (9) from the main centrifuge (60) is fluidly coupled to an inlet of the second channel (24) of the heat exchanger (20).

A grease recovery unit, particularly for use in a food service establishment, for separating oily substances from water in waste food has an inlet 2 connected via a valve 26 to a strainer 24, where solids are collected. The valve 26 is interlinked with the strainer 24 so that the valve closes the inlet 2 when the strainer 24 is removed. Oily substances from the food waste is separated from water by at least one hydrocyclone 30. Water that is separated is passed out of outlet 4 and the oily substances are passed into a collector 3. Fatty substances in the oily substances are held in a fluid state by a silicon heater pad 60.

An agitator is capable of more efficiently applying a shear force to a treatment target fluid through the action of an intermittent jet flow. The agitator includes a rotor which includes blade, and a screen concentric therewith, the rotation of at least the rotor causing a treatment target fluid to pass through slits in the screen to become an intermittent get stream, which is discharged from inside the screen to the outside thereof, wherein the agitator is characterized in that: the screen has a cylindrical shape with a circular cross section; openings of the slits provided in an inner surface of the screen constitute flow intake openings; openings of the slits provided in an outer surface of the screen constitute flow discharge openings; spaces between the flow intake openings and the flow discharge openings constitute slit space; and the circumferential-directional width (So) of the flow discharge openings and the circumferential-directional width (Si) of the flow intake openings are greater than the circumferential-directional width (Sm) of the slit spaces.

This disclosure describes methods to separate solids from liquids in a production facility. A process separates components in the process stream by using a mechanical device to separate the solids from the liquids based on a density difference. The process produces the liquids and solids, which may be further processed to create valuable animal feed products.

The present disclosure provides a method of separating hematopoietic stem cells from umbilical cord blood, including the following steps: a hydroxyethyl starch solution is added into cord blood and mixed uniformly, then centrifuged to get an upper liquid layer and a lower erythrocyte layer; the liquid in the upper layer and the erythrocytes in the lower layer are centrifuged respectively; an upper plasma layer and a basal cell layer are obtained after the centrifugation of the upper liquid layer, the cells in the basal layer are resuspended; a superficial buffy-coat layer is obtained after the centrifugation of the erythrocytes, and the superficial buffy-coat layer is metered with the plasma in the upper layer, and then centrifuged to get a lower cell layer, which is precipitated to remove erythrocytes and then resuspended; the above resuspended cells are added into freezing medium, mixed uniformly and then stored in a liquid nitrogen tank.

A mechanical separator for separating a fluid sample into first and second phases within a collection container is disclosed. The mechanical separator may have a separator body having a through-hole defined therein, with the through-hole adapted for allowing fluid to pass therethrough. The separator body includes a float, having a first density, and a ballast, having a second density greater than the first density. A portion of the float is connected to a portion of the ballast. Optionally, the float may include a first extended tab adjacent a first opening of the through-hole and a second extended tab adjacent the second opening of the through-hole. In certain configurations, the separator body also includes an extended tab band disposed about an outer surface of the float. The separator body may also include an engagement band circumferentially disposed about at least a portion of the separator body.