Blood in a separation chamber is separated into a red blood cell layer, a plasma constituent, and a mononuclear cell-containing layer. A portion of the plasma constituent exits the chamber via a plasma outlet, while a first portion of the red blood cell layer exits via a red blood cell outlet. A second portion of the red blood cell layer exits the chamber via the red blood cell outlet and is collected. At least a portion of the collected red blood cell layer may then be conveyed to the chamber via the red blood cell outlet to convey at least a portion of the mononuclear cell-containing layer out of the chamber via the plasma outlet for collection. A second portion of the plasma constituent may be conveyed out of the chamber via the plasma outlet to more fully collect the mononuclear cell-containing layer without the use of collected plasma.

Methods and systems for treating brine to produce distilled water and dried NaCl are disclosed. The brine enters a crystallization plant and is heated. Once heated, the brine is circulated to an evaporator. The evaporator increases the concentration of NaCl in the brine to a point about the super saturation level. Once above the super saturation level, NaCl crystals are formed. The larger crystals are circulated to a centrifuge for drying and the smaller crystals are recirculated through the evaporator for continued growth. The NaCl crystals are dried in the centrifuge.

Provided herein is a kit for isolation of platelet-rich plasma, comprising a first isolation tube and a second isolation tube. The first isolation tube comprises a first opening and a second opening, and the second isolation tube comprises a first portion, a second portion, and a filter; wherein the first portion has a third opening, the filter is disposed between the first portion and the second portion, the second opening has a diameter in a range of 1 to 5 mm and the filter has a pore size in a range of 1.5 to 4 μm.

A method of separating sludge which involves adding an insoluble mineral colloidal suspension into an industrial sludge to destabilize the industrial sludge and separating destabilized components of the industrial sludge. The insoluble mineral colloidal suspension includes magnesium hydroxide. In an alternative embodiment dry finely divided magnesium hydroxide can be added and then dispersed into an industrial sludge. Conventional flocculants and/or coagulants can also be added. Conventional physical separation processes can be used to separate the destabilized industrial sludge.

A water purification system with a centrifugal system and a frictional heating system consists of a centrifugal unit, a cavitation unit, a cooling condenser, a vertical shaft, and a pitot tube. The centrifugal unit and the cavitation unit are mounted along the vertical shaft so that the rotational movement of the vertical shaft is transferred onto the centrifugal unit and the cavitation unit. Non-potable water is directed into the centrifugal unit to separate heavy solids. Less populated water from the centrifugal unit is transferred to the cavitation unit via the pitot tube. The cavitation unit uses friction to generate phase change in the volume of less populated water which is then directed to the cooling condenser to produce potable water.

The invention provides an apparatus for separating components of a fluid stream, the apparatus comprising: (a) a support structure on which is mounted a rotatable centrifugal separator chamber in which separation of the components of the fluid stream occurs; (b) a fluid inlet for introducing a pressurised source of the fluid stream to be separated into the centrifugal separator chamber; (c) a fluid outlet for collecting one or more separated components of the fluid stream; (d) a vortex-creating device which rotates in order to introduce a vortex to the fluid stream in the centrifugal separation chamber thereby to bring about centrifugal separation of the components of the fluid stream; and wherein the centrifugal separator chamber and vortex-creating device are configured to rotate independently of each other.The invention also provides a method for separating components of a suspension, the method comprising passing the suspension through an apparatus as described herein.

Disclosed is a bioprocessing system comprising apparatus (200) including a centrifugal separation housing (210) having a temperature controllable compartment (215) for removably accepting a separation chamber (50), the apparatus further comprising at least one mixing station (250) for supporting one or more fluid storage vessels (10, 20, 30, 40), the station including a temperature controllable area (252) for increasing or decreasing the temperature of the contents of the or each supported vessel. The system further includes a disposable fluidic arrangement (100) including a centrifugal separation chamber (50) removably mountable within the compartment (215) and having one or more ports (52) allowing fluid ingress into, or egress out of the chamber, via the one or more ports in use, said ports being in fluid communication with one or more of said fluid storage vessels via fluid conduits (12, 22, 32, 42) and via one or more valve arrangement.

A device that allows for either fat graft preparation or cell fraction harvest is disclosed. The device includes a first centrifuge tube configured to receive and process a biological substance, the first centrifuge tube comprising an upper cylindrical portion and a lower conical portion, a sterile tissue inlet fitting, at least one sterile processing fluid inlet fitting, a sterile suction fitting, and at least one sterile extraction port connected to a first extraction tube. The first centrifuge tube further includes an internal space including a screen being positioned therein, the screen being configured to divide the internal space in half, and a filter positioned therein, the filter being positioned below the screen in the lower conical portion of the first centrifuge tube. The device may further include a second centrifuge tube configured to receive and further process the biological substance from the first centrifuge tube. The second centrifuge tube has at least one sterile fitting, wherein the second centrifuge tube is releasably connected via the at least one sterile fitting to one of the at least one sterile extraction ports of the first centrifuge tube.

The invention in at least one embodiment includes a system for treating water having an intake module, a vortex module, a disk-pack module, and a motor module. In at least one embodiment, a water system includes vortex housing having an interior wall defining a vortex chamber in fluid communication with the expansion chamber of said disk-pack turbine and inlets into the vortex chamber, and the vortex chamber having an upper section with a bowl or modified concave hyperbolic shape into which fluid is received from said inlets and a lower section with a conical or funnel shape with a steep vertical angle of change that opens into expansion chamber. In at least one further embodiment, the disk-pack module includes a disk-pack turbine having a plurality of disks having at least one waveform present on at least one of the disks.

A method is provided for preventing or reducing the formation of monochloropropanediols (MCPDs) or monochloropropanediol esters (MCPDEs) in triacylglyceride oil, comprising the steps: (a) concentrating insoluble components in liquid triacylglyceride oil by (i) applying a 5 centrifugational force on the triacylglyceride oil whilst maintaining the triacylglyceride oil above its melting temperature; and/or (ii) allowing the insoluble components to settle by gravitational force whilst maintaining the triacylglyceride oil above its melting temperature; (b) separating the triacylglyceride oil from the insoluble components; (c) optionally applying additional refining steps and (d) applying heat treatment to the triacylglyceride oil. A purified 10 triacylglyceride oil obtainable by the method of the invention is also provided.