There is described the processing seawater in a subsea facility on the seabed in various methods and apparatus. In various examples, the facility is coupled to at least one well, is configured to provide the well with water to be injected into at least one formation of the well, and comprises filter elements arranged in housings, the filter elements being configured for ultrafiltration or microfiltration. In such examples, treated seawater in at least one of the housings is filtered using at least one filter element, producing thereby filtered water, and at least one filter element in at least one other of the filter housings is cleaned by backwashing performed using at least some of the produced filtered water.

A method for rapid isolation of a biological compound (e.g. protein) from an aqueous sample is described herein. The method uses a porous hydrophobic membrane that has an average pore size significantly greater than the size of the biological compound. The method permits the biological compound to attach to the membrane while the aqueous solvent rapidly moves through the membrane under the application of a vacuum. The biological compound that is attached to the membrane can be washed, optionally digested, and eluted for analysis.

The present invention relates to a method for recovering a rare metal salt, the method including: an acid treatment step of obtaining a rare metal-containing acidic aqueous solution by bringing a material including a monovalent rare metal and a polyvalent rare metal into contact with an acidic aqueous solution; a separation step of obtaining permeated water including the monovalent rare metal and non-permeated water including the polyvalent rare metal from the rare metal-containing acidic aqueous solution by using a nanofiltration membrane satisfying the condition (1); and a concentration step of obtaining non-permeated water having a higher concentration of the monovalent rare metal and permeated water having a lower concentration of the monovalent rare metal than that of the permeated water in the separation step, by using a reverse osmosis membrane.

A portable system for producing a formulation comprising lipid nanoparticle (LNP)-encapsulated RNA includes: a first sub-system comprising multiple drug substance formulation modules, the first sub-system comprising: a transcription module for forming an RNA solution via in vitro transcription; and a second sub-system operatively downstream of the first sub-system comprising multiple drug product formation modules, the second sub-system comprising: an LNP formulation module for producing a first RNA-LNP preparation from the RNA solution, wherein each of the transcription module and the LNP formulation module is contained within a separate standard shipping container.

A method for exosome separation and extraction by stacked centrifugal filtration. It is used in molecular biology and clinical examination and comprises an exosome separation and extraction kit consisting of the stacked centrifugal filtration device, an incubation buffer and a protease K. The sample to be tested is incubated at room temperature using the incubation buffer and an appropriate amount of protease K, followed by centrifugation in a centrifuge matching the stacked centrifugal filtration device. After mixing thoroughly, the retained liquid in the ultrafiltration tube is collected to obtain the exosomes. The method needs no large experimental equipments except for a centrifuge, which has a low cost and which is convenient and fast, with short operation time and the possibility of carrying out parallel operations with a large number of samples. The high purity exosomes obtained by the method can meet the demand of large-scale clinical applications.

The present disclosure relates generally to the manufacturing of gene therapy products, and specifically to methods of purifying an enveloped virus from a cell culture fluid, comprising an endonuclease and/or anion exchange chromatography.

Systems and methods for filtering materials from biologic fluids are discussed. Embodiments may be used to filter cerebrospinal fluid (CSF) from a human or animal subject. In an example, CSF is separated into a permeate and retentate using a tangential flow filter. The retentate is filtered again and then returned to the subject with the permeate. During operation of the system, various parameters may be modified, such as flow rate and waste rate.

The present invention relates to a separation of viruses of an essentially spherical shape from viruses with a rod-like shape that are comprised in a sample, wherein the sample comprising the viruses is subjected to filtration.

A method of filtering a liquid feed is described, comprising passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers. A method of filtering a liquid feed is also described comprising passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods of the invention can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

A mobile device for biological treatment of bioreactor-type wastewater with a submerged membrane enabling treatment of greywater and blackwater has an inlet duct for effluent to be treated and an outlet duct for treated and filtered water connected to a permeate pump. The device includes a container, the interior volume of which has a parallelepiped appearance with two large vertical lateral sides, and a membrane filter having an assembly of parallel, planar filtration membranes also with a vertical appearance. The membranes are connected to a downstream collector collecting the filtered water and connected to the outlet duct. The permeate pump ensures a transmembrane flow less than the subcritical flow. At least one diffuser of fine air bubbles is located at the base of each column. Each diffuser is connected to a regulating solenoid valve and to pump, ensuring therein an airflow greater than or equal to 10 Nm.sup.3/h per diffuser.