Protein separation can be provided based on a High Performance Countercurrent Membrane Purification (HPCMP), which can exploit highly selective diffusive transport across thin walls of a hollow fiber membrane for separation of proteins. Embodiments of an HPCMP system can separate mixtures of multiple proteins (e.g. separate BSA and Mb) or other biological material and provide high yields (e.g. achieving greater than 98% yield of both proteins with purification factors greater than 100-fold, etc.). Embodiments of a HPCMP system can be configured for high resolution separations in the preparation of biopharmaceuticals and natural protein products. Other embodiments can be utilized in other protein separation environments or biological material separation environments.

The present disclosure provides a filtration media comprising a combination of (i) a hollow fiber membrane and (ii) adsorption media. The disclosure further provides a device, the device comprising a filtration media, which filtration media includes a combination of (i) a hollow fiber membrane and (ii) adsorption media.

Parallel plate devices for hemofiltration or hemodialysis are provided. A parallel plate device includes a parallel plate assembly having an aligned stack of stackable plate subunits, each stackable plate subunit having a through channel for blood, where the blood channels are opened up at opposite ends of the parallel plate assembly. The parallel plate assembly is configured to form filtrate/dialysate channels interleaved with the blood channels, adjacent channels being separated by a silicon nanoporous filtration membrane. A blood conduit adaptor is attached to the parallel plate assembly at each of the ends, and is configured to distribute blood to or collect blood from the blood channels. Also provided are systems and methods for using the parallel plate devices.

A method for producing human collagen structures with controlled characteristics, having the following stages: a) Tissue conditioning b) Pre-treatment; b1) final washings with distilled water; c) extraction by enzymatic hydrolysis by subjecting the tissue to a solution of acetic acid with pepsin; d) precipitation, where the resulting collagen solution is brought to a high concentration by adding sodium chloride and where the fibers recovered from the sieve are solubilized again in an acetic acid solution; e) dynamic dialysis to purify the solution from the excess of salt present in the collagen solution; f) lyophilization at 40 C. and a vacuum pressure of 0.04 mbar (4 Pa); g) molding or second lyophilization, where a concentration is chosen and collagen is solubilized in an acetic acid solution; once solubilized, collagen is placed in molds to generate the desired structure and once again, the solution is lyophilized; h) crosslinking, where the collagen pieces are subjected to a formaldehyde vapor atmosphere in a crosslinking apparatus and i) pressing, where the collagen structure is subjected to a mechanical force of 400-5000 N compacting to a value of 0.01-10 mm and increasing its fibrillar density.

The present disclosure relates to semipermeable membranes which are suitable for blood purification, e.g. by hemodialysis, which have an increased ability to remove larger molecules while at the same time effectively retaining albumin. The membranes are characterized by a molecular retention onset (MWRO) of between 9.0 kD and 14.5 kD and a molecular weight cut-off (MWCO) of between 55 kD and 130 kD as determined by dextran sieving curves and can be prepared by industrially feasible processes excluding a treatment with salt before drying. The invention therefore also relates to a process for the production of the membranes and to their use in medical applications.

A membrane for the purification of blood, or a dialysis membrane, in hollow-fiber membrane or flat membrane geometry, made of a composite assembled from at least a base membrane based on at least one polysulfone or a polyphenylsulfone with at least one pore-forming hydrophilic additive and at least one functional layer arranged on the base membrane, whereby the functional layer is formed from at least one polymeric polycationic bonding agent and at least one polymeric polyanion, whereby the base membrane is made of a material which is selected from: a polysulfone [PSU], a sulfonated polysulfone [SPSU], a polyethersulfone [PES], a sulfonated polyethersulfone [SPES], a polyphenylsulfone [PPSU], a sulfonated polyphenylsulfone [SPPSU]; and mixtures of these.

Dialysis devices include a frame defined by a plurality of sidewalls that are impermeable to a sample being dialyzed, a pair of dialysis membranes that are each associated with an opposing face of the plurality of sidewalls such that the plurality of sidewalls and the pair of dialysis membranes define a sample chamber, an outer shell surrounding at least a portion of the pair of dialysis membranes, and a cap selectively associated with the sample chamber. The cap can be selectively associated with the sample chamber via an attachment mechanism that is configured to provide aural and/or haptic feedback when the cap forms a tight association with the sample chamber. The cap can be a sensor cap having one or more probes for measuring at least one property of fluid inside and/or outside the sample chamber and a transmitter for transmitting data captured at the probe(s) to a destination device.

A method for performing a peritoneal dialysis therapy includes performing a plurality of peritoneal dialysis cycles for a patient and tracking an amount of dialysis fluid provided by at least one dialysis fluid pump during the plurality of peritoneal dialysis cycles. The method also includes determining an amount of ultrafiltrate (UF) removed from the patient based on the amount of dialysis fluid provided by the at least one dialysis fluid pump. The method further includes updating a UF trend using previous amounts of UF removed from the patient and the amount of UF removed from the patient during the most recent dialysis treatment and generating an alert if the UF trend changes by more than a preset percentage.

The present disclosure relates to semipermeable membranes based on acrylonitrile copolymers capable of adsorbing bacteria from fluids, methods of producing such membranes, and devices comprising such membranes.

A disposable cartridge for use in a hemodialysis machine has a blood flow path for carrying a volume of blood to be treated in a dialyser and a dialysate flow path, isolated from the blood flow path, for delivering a flow of dialysate solution through the dialyser. The cartridge is received in an engine section of the machine. The engine section has first and second platens which close when the cartridge is inserted to retain the cartridge. Actuators and sensors arranged on the second platen control operation of the cartridge.