The present invention relates to separation of desired target products from biological, plant, and waste-type material, wherein the desired target products include renewable fuels such as ethanol, biobutanol, and biodiesel, wherein the separation is conducted with a cross-flow filtration system having the ability to separate desired products from both non-viscous and viscous medium.

The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly.

A method of filtering a liquid feed is described, comprising passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers. A method of filtering a liquid feed is also described comprising passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods of the invention can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

A humidifier having a multiplicity of metallic flow plates and a multiplicity of water transfer membranes, in particular for humidifying process gas for an electrochemical system or for HVAC applications is described. The humidifier has a construction in which the metallic flow plates and the water transfer membranes are arranged such that in each case at most one of the single-layer flow plates is arranged between adjacent water transfer membranes of the stack; and at least one of the water transfer membranes is in each case accommodated in a sealing manner, in particular clamped in a sealing manner, between the mutually facing sealing lines of adjacent flow plates of the stack. The humidifier has lower production costs, greater efficiency and lower weight.

A method of operating an immersed microporous membrane module includes a step of monitoring membrane performance to sense the onset of sludging in the module. Differences in permeability between permeation in backwashes, or trends in permeability during backwashing and permeability during permeation, or both, are monitored. Solid deposits formed during the onset of sludging may be removed with an in situ de-sludging process. For example, the deposits may be removed by stopping permeation while aerating the module, optionally at an increased rate. At other times, the module is optionally aerated while permeate is withdrawn at an aeration rate correlated to flux. The method may be used in particular with a membrane module having parallel textured flat sheet membranes suspended between a pair of vertically oriented headers. An aerator may be made from an open bottomed channel having an array of holes rising and concentrated towards the center of the channel.

Microstructure separation filters are provided herein, as well as chromatography and other separation devices. An exemplary filter device includes a microstructure filter has a plurality of layers of alternating sacrificial and/or structural material which have been etched to create inlet channels and outlet channels. Adjacent ones of the inlet channels and the outlet channels are spaced apart from one another by cross channels that filter a fluid from the inlet channels to the outlet channels. The cross channels include filter features formed by etching away of a portion of the layers. The device also includes a housing configured to receive the microstructure filter.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

A modular structure for a mitigating evaporative fuel emissions, such as for an automobile, is described. The structure may include a plurality of frames and membranes for flowing fuel vapor and reducing the emission of hydrocarbon therefrom. The structure may include flow guides that provide a meandering flow path for both the fuel vapor and a permeate. A flow guide providing parallel flow paths is also described.

The present disclosure provides for devices, systems and methods for fractionation and concentration of particles from a fluid sample. This includes a cartridge containing staged filters having porous surface in series of decreasing pore size for capture of particles from a fluid sample; and a permeate pressure source in fluid communication with the cartridge; wherein the particles are eluted from the porous surfaces and dispensed in a reduced fluid volume.

A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.