A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

A microfluidic assembly can include a first microchannel substrate defining one or more first microchannels, a second microchannel substrate defining one or more second microchannels. The assembly can further include a membrane positioned between the first and second microchannel substrates and comprising a first polymeric layer, a second polymeric layer, and one or more graphene layers disposed between the first and second polymeric layers. At least a portion of the first microchannels can overlap at least a portion of the second microchannels such that, when a first fluid is present in the first microchannels and a second fluid is present in the second microchannels, the first fluid and the second fluid contact opposite sides of the membrane.

A flow plate for a humidifier, having flow fields on both mutually opposite flat sides of the flow plate is described. The flow plate proposed here has a multiplicity of through-openings, wherein the through-openings are designed such that a perpendicular projection of the through openings onto a plane oriented parallel to the plane of the flat surface of the flow plate has a non-vanishing area, so that gas passing through the through-openings effects, at least in part, mixing of a gas guided in the flow fields perpendicular to the plane of the flat surface of the flow plate. The through-openings improve the power of the humidifier, thus permitting a lower number of flow plates and therefore a more lightweight and more compact design.

The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly.

Devices and methods are provided that permit efficient and selective separation of liquid biological specimens into at least two constituent components to facilitate subsequent quantitative and qualitative analysis on at least one analyte of interest in at least one of the components. The devices generally include one or more sample deposition regions supported on a base. Each sample deposition region includes a separation membrane for separating the liquid biological specimen into two different fractions. The first fraction is trapped by the separation membrane while the second fraction passes through the separation membrane and into a respective collection membrane. The separation and collection membranes are easily separable from the devices and can be utilized for further processing and analysis.

A piece of substrate material is formed under heat and pressure against a cavity into a shaped substrate sheet having one or more depressions. Two substrate sheets are bonded together to form a substrate wherein the one or more depressions form one or more interior channels. The substrate, if not formed with pre-coated substrate material, is coated with a dope and quenched to form a filtering membrane. A plurality of membranes may be placed side by side to form a bundle with permeating ends of the membrane, which are open to the one or more interior channels, separated by gaps or spacers. The bundle is connected to a header to produce a module. The module can be assembled into a cassette.

A composite membrane and moisture adjustment module using the same is disclosed. The composite membrane includes a moisture-permeable resin layer interposed between porous membranes that constitute a pair; and the mean thickness of the moisture-permeable resin layer is 5 μm or less.

A feeding head assembly and a chemical release system for eliminating scale conditions and maintaining a neutral pH in potable water is disclosed. The feeding head assembly may include a composite membrane system comprising at least one semi-permeable layer and at least one filter layer. A chemical release system may include a cartridge or vessel. The cartridge may include a hollow body with two opposing open ends. A time-release pouch may be located within the hollow body and holds a composition that mixes with potable water to eliminate scale conditions and maintain a neutral pH in the potable water. A feeding head assembly may be coupled within the hollow body between the time-release pouch and one open end of the hollow body.

A composite media includes a stack with 20 or more layers, each layer including a substrate with a first major surface and an opposing second major surface; and a fiber layer deposited onto the first major surface of the substrate and including polymeric fibers with a diameter of 100 nm to 1.5 μm. Each fiber layer independently has a thickness of 5 μm to 100 μm, and each layer independently has a pore size of 0.1 μm to 10 μm. A filter may include a housing and the composite media disposed within the housing. The filter may be a syringe filter.

A method of filtering a liquid feed is described, comprising passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers. A method of filtering a liquid feed is also described comprising passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods of the invention can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.