Disclosed herein are spacers having baffle designs and perforations for efficiently and effectively separating one or more membrane layers a membrane filtration system. The spacer includes a body formed at least in part by baffles that are interconnected, and the baffles define boundaries of openings or apertures through a thickness direction of the body of the spacer. Alternatively or additionally, passages or perforations may be present in the spacer layer or baffles for fluid flow there through, with the passages and baffles having a numerous different shapes and sizes.

A self-contained filtration assembly with a self-contained filtration cassette therein. The cassette may be formed from one or more layers membranes and spacers (spacers, screens, or combinations thereof) all having the same general shape. The cassette may have a feed inlet path and a retentate outlet path extending through the layers of the cassette within the perimeter of the cassette, and a permeate outlet path between the cassette exterior and the interior of the cassette housing. The housing may have a generally circular or elliptical cross-section. A clamping assembly may hold together the components of the housing, and may be elongated in a direction along the flow path of the feedstream through the cassette. Various port-defining zones defined in the layers of the cassette may be sealed in a manner facilitating assembly of the cassette as well as isolation of the flow paths therein.

Disclosed are apparatus and methods for blood and other biological fluid purification using a membrane with cell containing vascular channel systems and filtration channel systems. Also disclosed are methods of making the apparatus as well as methods of making membranes.

A device including a housing having a first side, a second side opposed to the first side, and a wall disposed between the first side and the second side. The wall forms at least a partially enclosed space inside the housing. The device also includes a membrane mounted to the wall inside the housing. The device also includes a drop pipe disposed inside the housing and through or around the membrane, the drop pipe having a proximal end and a distal end, the distal end being closer to the second side than the proximal end. The device also includes a diffuser connected to the distal end of the drop pipe.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

An induced symbiotic osmosis system and method for treating produced fluids from a hydraulic fracturing process or system for symbiotic fluids fractionation, salinity power generation, brines and salts solution reverse osmosis. The system includes a reverse osmosis membrane assembly to create potable water from produced water. The membrane assembly includes a hollow fiber or flat sheet membrane and headers to transfer desalinated water therefrom. The system can include an electro coagulation system, an ultra or nano filtration system, and a desalter to treat the produced water or brine. A heat exchanger can be positioned between adjacent reverse osmosis membrane assemblies. An osmotic power generation unit can create electrical power by receiving and utilizing produce water and brine water from a brine storage. The system reduces the release of global warming contributing gases associated with hydraulic fracturing, while producing potable water and power.

The present disclosure is directed to affinity chromatography devices that include a fibrillated heat treated polymer membrane that contains therein inorganic particles that separate a targeted molecule from an aqueous mixture containing the targeted molecule. The targeted molecule includes proteins, antibodies, viral vectors, nucleic acids, and combinations thereof. The inorganic particles may be spherical or irregular in shape. A blend or combination of various sizes and/or shapes of inorganic particles may be utilized. An affinity ligand may be bonded to the inorganic particles and/or to the fibrillated polymer membrane. The affinity chromatography device may be repeatedly used and may be cleaned between uses. In some embodiments, the affinity chromatography devices separate nucleic acids (e.g., mRNA) and viral vectors (e.g., adeno-associated virus) from the aqueous mixture. Manifolds containing multiple affinity chromatography devices in a parallel configuration and multiple manifolds in a parallel configuration are also disclosed.

The invention relates to a modular flow system having a plurality of frame elements (101, 102) configured to be combined together to form a stack for forming afunctional member such as in particular a membrane distillation stage, a vapor generator, a condenser, a heat exchanger, a filter and/or a pervaporation stage, wherein the frame elements (101, 102) each include: an outer frame (39) and an inner frame (43), the inner frame (43) encasing a central inner region (40) and being surrounded by the outer frame (39), passage openings (13 to 16) and vapor and/or liquid channels (17, 18) arranged between the outer frame (39) and the inner frame (43), wherein at least one of the vapor and/or liquid channels (17, 18) comprises at least one internal strut member (48) extending between the inner frame (43) and the outer frame (39).

A filtration cassette including a multilaminate array of sheets including filter sheets alternating with permeate sheet members and retentate sheet members, and a cross-flow filter device comprising a multiplicity of stacked filtration cassettes of such type. The improvements associated with the filtration cassettes described herein include, but are not limited to, at least one of: reinforced inlet(s) providing for longevity and improved cleanability of the cassettes; spacer permeate screens to limit throughput restriction; stainless steel or otherwise stiffened permeate sheets to prevent movement and increase flux; and stainless steel permeate sheets that can be used with ultrasonic transmission to minimize fouling of the filter sheets and extend cleaning cycles. Advantageously, the filtration cassettes are more resistant to higher temperatures than the filtration cassettes of the prior art.

An apparatus for treating a biological fluid, comprising a plurality of filtration devices. In some embodiments, each of the plurality of filtration devices comprising at least one inlet and at least one outlet; and a first insert plate and a second insert plate opposite the first insert plate, wherein the plurality of filtration devices is disposed therebetween. In some embodiments, each filtration device has a vent port. A manifold for each of the inlets, outlets, and vent ports is optionally fluidly connected. In another embodiment, hose barb connectors or the like may be protected from damage and/or exposure by being recessed in the device or positioned at a periphery of the device. Embodiments include apparatus and methods to achieve dripless connect/disconnect and to reduce the number of sterile-to-sterile connections.