A connection mechanism for a suction device for vacuum membrane filtration applications, includes a support for receiving a membrane filter or a filtration base, a cavity formed below the membrane filter or the filtration base, a suction duct which opens centrally into the cavity, and a ventilation duct which opens laterally into the cavity. The connection mechanism further includes a closing element which can be moved into several switching positions and which can block or unblock both the suction duct and the ventilation duct depending on the switching positions.

Devices and methods herein remove water from human or animal biological waste fluids using one or more forward osmosis filters. The devices allow for the volume of liquid or semi-liquid waste, including potentially infectious liquid waste, to be filtered to reduce potential exposure of healthcare staff to infectious liquid waste. On a hospital, healthcare staff, or individual patient basis, removing water and concentrating the waste can reduce challenges in management and disposal of the waste. Devices herein use forward osmosis to manage and filter, using one or more suitably sized filter(s), biological fluid exudate from wounds. The devices can be constructed to transport water present in the exudate away from a wound. The wound treatment devices herein not only allow for fluid from wounds to be filtered but also provide structures that can protect wounds from external contaminants, including bacteria and viruses. The wound treatment devices can be incorporated into negative pressure wound therapy systems, if desired.

The present technology provides micro fabricated filtration devices, methods of making such devices, and uses for microfabricated filtration devices. The devices may allow diffusion to occur between two fluids with improved transport resistance characteristics as compared to conventional filtration devices. The devices may include a compound structure that includes a porous membrane overlying a support structure. The support structure may define a cavity and a plurality of recesses formed in a way that can allow modified convective flow of a first fluid to provide improved diffusive transport between the first fluid and a second fluid through the membrane.

Embodiments of the present disclosure relate generally to dead-end filtration systems and, more particularly, to pulse-modulated periodic backflush systems and methods for clearing fouling layers in dead-end filtration systems. In some embodiments, a controller may control the flow of fluid in the system from cycling from a forward flow to a reverse flow. In some embodiments, the controller may cycle from forward to reverse flow based on a volumetric flow ratio. Embodiments of the present disclosure describe optimal volumetric flow ratios for optimizing the break of cake in a dead-end filtration system. Embodiments of the present disclosure describe optimal volumetric flow ratios for optimizing recovery percentages of targeted particles.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present disclosure is directed to cross-flow membrane emulsification devices. The devices disclosed herein can have a continuous phase plate, a dispersed phase plate, an outlet, and a chamber. The chamber is located between the continuous phase plate and the dispersed phase plate and is bisected by a membrane with a plurality of pores. The chamber can include at least one channel on a first side of the membrane formed from at least one groove in the continuous phase plate and the membrane. In addition, the chamber can also include a cavity on a second side of the membrane formed in the dispersed phase plate.

A filtration filter that includes a filter section having a plurality of through-holes passing through a first main surface and constructed such that objects to be filtered contained in a liquid are captured and a second main surface opposite the first main surface; and a frame section surrounding an outer periphery of the filter section, wherein a first film thickness at a center of the filter section is larger than a second film thickness of the filter section at a position closer to the frame section than the center of the filter section.

Filtration head (1) for a filter system, comprising base (2) including filter support (3) for removable filter element (4) and a fluid path from one side of filter element (4) on filter support (3), through filter element (4), and to drainage outlet (5) of base (2), and holder device (6) for removably attaching funnel (7) to base (2) to form a sample fluid reservoir on one side of filter element (4), wherein holder device (6) comprises ring-shaped element (8) configured to extend about bottom peripheral flange (7a) surrounding a lower end of funnel (7) and to selectively apply a biasing force on a radially outward protruding portion (7b) of bottom peripheral flange (7a) of funnel (7) to press funnel (7) against base (2). The filtration head allows the use of cardboard funnels without risk of liquid leakage between the funnel and the base.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

This invention describes a method and a device for efficient isolation of extracellular vesicles from animal and human biological fluids, as well as from culture fluid using equipment of standard diagnostic laboratories, that is, without the use of ultracentrifugation. These method and device can be applied for the diagnosis of various human diseases, as well as for therapeutic purposes, if the purified vesicles are used as an agent for drug delivery to the cells of the body. The device for the purification of extracellular vesicles contains at least two membrane filters: the first filter containing a membrane with pore sizes in the range from 400 to 600 nm, connected to the second filter containing a membrane with pores in the range from 95 to 200 nm. At the same time, the membranes of these filters are made of materials that practically do not bind biological polymers.