Fluid treatment assemblies comprising a plurality of adjustable tensioning rod assemblies comprising tensioning rods having a tensioning nut and a locking nut threadably attached to the tensioning rods, and methods of adjusting the tension on the assemblies, are provided.

Methods and systems are provided for a stem cell organ device including an array of alternating stem cell channels and fluid channels. In one example, a method may include loading a stem cell channel with stem cells and flowing blood through a fluid channel in order to allow an exchange of molecules between the stem cells and the blood.

The present invention relates to portable filtration units including filter components mated with retentate and permeate flow channels wherein particle containing solutions are introduced into the portable filtration units and contacted with the filter components for filtration thereby producing a retentate outflow and a permeate outflow for capture of desired end product.

Method that includes providing a phase-separation device having a porous membrane with a filter surface. The filter surface has a non-planar contour that forms a receiving cavity. The method also includes providing a liquid mixture into the receiving cavity of the porous membrane. The liquid mixture includes a polar liquid and a non-polar liquid that are immiscible with respect to each other. The filter surface along the receiving cavity has a surface energy that impedes flow of the polar liquid through the filter surface and permit flow of the non-polar liquid into the porous membrane. The method also includes permitting the non-polar liquid to flow into the porous membrane. The polar liquid forms a droplet within the receiving cavity as the non-polar liquid flows into the porous membrane.

Devices, methods, and kits directed towards collecting and preparing cells using a separable sample collection layer may be configured to collect or treat cells from a liquid sample with mechanisms for easy transfer of the cells prior to analysis or imaging. The separable sample collection layer may comprise a porous membrane that cells may be collected on, and one or more support layers comprising tape with one or more adhesive coatings and release liner. The devices, methods and kits may be configured with support layers comprising cutouts that form vertically or horizontally oriented microchannels for efficiently removing undesirable liquid. Following collection and/or treatment, cells collected onto the porous membrane may be adhered to another surface for further processing or analysis.

The present invention relates to a centrifugal force-based nanoparticle separation apparatus and method. Specifically, the present invention is based on having a low centrifugal force and a small size, and may thus separate nanovesicles unrelated to antibody specificity in a short time and without using an ultracentrifuge. Further, the present invention requires no additional professional personnel and enables accurate fluid measurement by integrating and automating all processes after sample injection, and may thus reduce the loss of nanovesicles.

Ultra-thin nanometer-sealer freestanding polymeric membranes and methods for producing ultra-thin nanometer-scale freestanding recast membranes and ultra-thin nanometer-scale freestanding cross-linked membranes with solid internal backbone are disclosed.

A microporous structure includes an array of nano wires and a coating about the nano wires of the array. The coating defines pores between the nano wires.

A microfluidic purification device for exosomes purification is disclosed that has high speed, high viability and efficiency plus re-cycling of cells for regrowth of exosomes. The microfluidic purification device contains coarse filtering, and/or medium filtering plus fine filter with the medium/fine filter made of MEMS semiconductor process. For high-speed operation, an ultrasound vibrator attached to input chamber/filter/output chamber assembly is also used that the vibration amplitude, duty cycle and duration can be controlled through controller. The MEMS filter is V-shaped and/or funnel shape made of silicon wafer by semiconductor process. For funnel shape MEMS filter, the exit hole size is between 0.2 ?m to 1 ?m suitable for exosomes filtering with high speed and high viability.

Microfluidic apparatuses including concentrators and buffer exchange regions that concentrate and exchange buffer. Also described are methods of passing a solution through a feed channel, filtering small molecules out of the feed channel by tangential flow filtration into a permeate channel adjacent to the first feed channel while maintaining a constant sheer rate relative to the membrane separating the feed channel from the permeate channel and exchanging buffer into the solution and concentrating the solution in a second region of the apparatus.