The present disclosure provides a cell separation apparatus for a bioreactor. The cell separation apparatus may be disposed outside the bioreactor and in fluid connection with the bioreactor, the cell separation apparatus may be in a shape of a box body, the cell separation apparatus may include a liquid buffer device including a first liquid cavity disposed in the box body; a filter device including a filter channel and a filter membrane disposed in the box body, the filter membrane may be disposed above the filter channel; and a first liquid channel may be configured in the box body to facilitate a fluid communication between the first liquid cavity and the filter channel. A power system for filtering and microfluidic channels are integrated in the cell separation apparatus that is of a box shape, thereby reducing the volume and production cost thereof.

A method for visualizing biofilm on a solid surface. The method comprises steps of:

(a) providing a solid surface which has been in contact with an aqueous medium and at least a portion of the solid surface potentially is covered with biofilm,
(b) maintaining the solid surface in a horizontal position and covering the solid surface with an aqueous dispersion of carbon particles,
(c) tilting the solid surface at an angle of at least 5° from horizontal to allow excess aqueous dispersion of carbon particles to flow from the surface,
(d) detecting any biofilm present on the solid surface by determining whether there are areas not covered by aqueous dispersion of carbon particles.

A water treatment device includes: a measure that repeats measurement of pressures on a primary side and a secondary side of a hollow fiber membrane module every minute time of three seconds or less in one or more steps of water filling, backwashing, pressure relieving, bubbling, and draining; and an estimater that estimates: abnormality of an internal state of the hollow fiber membrane module or of a peripheral device; a time required from a start to an end of the one or more steps; an effect of physical washing or chemical washing; or a future increase in an membrane differential pressure, based on a temporal transition of a measurement result of at least one of the pressures, or based on a difference in the temporal transition between a plurality of times of the step.

The present invention relates to a computer-readable recording medium having recorded thereon a clogging location determination program for a separation membrane module, in which, in order to determine a clogging location of a separation membrane module in a fresh water generation system for obtaining treated water by filtrating water to be treated by a separation membrane module having a separation membrane, a computer is caused to function as a clogging location determination means for determining a clogging location of the separation membrane module from a resistance in a lower part of the separation membrane module, a filtration resistance of a hollow-fiber membrane, and a resistance in an upper part of the separation membrane module.

A method for the detection and monitoring of surface fouling from the nanoscale as a predictive or real-time monitoring tool is provided. Also provided are devices for carrying out the method, and to uses of the method, in particular for optimising cleaning procedures of devices or components thereof fouled by flows of foulant.

Methods and devices for real-time detection of fouling chemistry are described herein. In one aspect, a method of detecting and characterizing fouling of a membrane used for separation in a fluid-based system can include illuminating the membrane with one or more light sources, collecting Raman spectroscopy data from the membrane, and based on the Raman data, determining at least one selected from the group consisting of: presence or absence of membrane fouling, severity of membrane fouling, and composition of the membrane fouling, where the Raman spectroscopy is selected from the group consisting of Coherent Anti-Stokes Raman Scattering (CARS), Stimulated Raman Scattering (SRS), and spontaneous Raman Scattering.

A system can include a filter assembly with a filter and a substance in the filter assembly, and at least one optical computing device having an integrated computational element which receives electromagnetic radiation from the substance. A method can include receiving electromagnetic radiation from a substance in a filter assembly, the electromagnetic radiation from the substance being received by at least one optical computing device having an integrated computational element, and the receiving being performed while a filter is positioned in the filter assembly. A detector may receive electromagnetic radiation from the integrated computational element and produce an output correlated to a characteristic of the substance. A mitigation technique may be selected, based on the detector output.

A predictive system for monitoring fouling of membranes of a desalination or water softening plant includes ultrafiltration (UF) membranes, reverse osmosis (RO) membranes, and/or nanofiltration (NF) membranes. In addition, the system includes one or more UF skids including a plurality of UF units. Each UF unit contains therein a plurality of UF membranes. Further, the system includes one or more RO/NF skids including one or more RO/NF arrays. Each of the one or more RO/NF arrays includes a plurality of RO units, with each RO unit containing therein a plurality of RO membranes, a plurality of NF units, with each NF unit containing therein a plurality of NF membranes, or a combination thereof. Still further, the system includes UF sensors and/or RO/NF sensors. The system also includes a controller comprising a processor in signal communication with the UF sensors and/or the RO/NF sensors.

Methods of detecting, quantifying and/or characterizing the fouling of a device from a combination of pressure and spectroscopic data are provided. The device can be any device containing components susceptible to fouling. Components can include membranes, pipes, or reactors. Suitable devices include membrane devices, heat exchangers, and chemical or bio-reactors. Membrane devices can include, for example, microfiltration devices, ultrafiltration devices, nanofiltration devices, reverse osmosis, forward osmosis, osmosis, reverse electrodialysis, electro-deionisation or membrane distillation devices. The methods can be applied to any type of membrane, including tubular, spiral, hollow fiber, flat sheet, and capillary membranes. The spectroscopic characterization can include measuring one or more of the absorption, fluorescence, or raman spectroscopic data of one or more foulants. The methods can allow for the early detection and/or characterization of fouling. The characterization can include determining the specific foulant(s) or type of foulant(s) present. The characterization of fouling can allow for the selection of an appropriate de-fouling method and timing.

A method for determining a fouling index ROFix in a filtration system includes supplying a liquid feed across a filtration membrane; measuring an initial filtrate flow rate Q.sub.0 of the liquid feed using a cross-flow filtration mode; measuring filtrate volumes V.sub.i at various corresponding times t.sub.i using the cross-flow filtration mode; fitting a straight line for the filtrate volumes V.sub.i and the corresponding times t.sub.i; and calculating a slope of the straight line. The slope or a parameter related to the slope is the ROFix index, and wherein the cross-flow filtration mode allows the liquid feed to exit the filtration system.