According to embodiments of the present disclosure, apparatuses, systems, and methods (e.g., an end-to-end manufacturing platform) for viral vectors are provided that include upstream production and downstream purification to the drug substance stage that is not impacted by the therapeutic vector payload.

A cord for supporting a biofilm has a plurality of yarns. At least one of the yarns comprises a plurality of hollow fiber gas transfer membranes. At least one of the yarns extends along the length of the cord generally in the shape of a spiral. Optionally, one or more of the yarns may comprise one or more reinforcing filaments. In some examples, a reinforcing yarn is wrapped around a core. A module may be made by potting a plurality of the cords in at least one header. A reactor may be made and operated by placing the module in a tank fed with water to be treated and supplying a gas to the module. In use, a biofilm covers the cords to form a membrane biofilm assembly.

A cord for supporting a biofilm has a plurality of yarns. At least one of the yarns comprises a plurality of hollow fiber gas transfer membranes. At least one of the yarns extends along the length of the cord generally in the shape of a spiral. Optionally, one or more of the yarns may comprise one or more reinforcing filaments. In some examples, a reinforcing yarn is wrapped around a core. A module may be made by potting a plurality of the cords in at least one header. A reactor may be made and operated by placing the module in a tank fed with water to be treated and supplying a gas to the module. In use, a biofilm covers the cords to form a membrane biofilm assembly.

The present disclosure provides extruded or spun, semi-permeable, porous hollow fibres, comprising covalent ester, thioester and/or amide crosslinked polypeptides as well as processes for their production. The hollow fibres may be produced from protein, protein extracts, and/or protein isolates derived from plants, animals, bacteria, algae, archaea, and/or fungi, and in certain embodiments are intended to be suitable for human and/or animal ingestion. In some embodiments, the hollow fibres may be designed to be used in the production of cartridges that are compatible with existing and/or novel bioreactor platforms, for harbouring cell cultures in cultured meat production.

An operation method of a hollow fiber membrane module is described, the operation method including: a filtration step of performing cross-flow filtration by supplying a feed to an outer surface side of a hollow fiber membrane, in which in the filtration step, a ratio of a viscosity .sub.f of the feed to a viscosity .sub.p of a filtrate satisfies a relation of .sub.f/.sub.p1.5, and a flow rate ratio of a flow rate v.sub.p of the filtrate to a flow rate v.sub.f of the feed satisfies a relation of 0.02v.sub.p/v.sub.f0.3.

A hollow-fiber membrane. The hollow-fiber membrane is made from a polymeric blend comprising an aromatic sulfone polymer and polyoxazoline, wherein the polymeric blend comprises from 27 wt. % to 30 wt. % aromatic sulfone polymer, based on the total weight of the polymeric blend; wherein the hollow-fiber membrane comprises an inner surface facing towards its lumen, an outer surface facing outwards and an intermediate wall having a wall thickness; wherein the hollow-fiber membrane is an integrally asymmetric, permeable hollow-fiber membrane.

The present disclosure relates to a process for manufacturing a sterilizing filter comprising a plurality of hollow fiber membranes having a large inner diameter.

Provided is a method for manufacturing a product, including a culture step of culturing cells in a culture solution in a tank having a capacity of 100 L or more, and a separation step of using a hollow fiber membrane filter comprising a plurality of hollow fiber membrane, each having a primary flow passage extending in a longitudinal direction, and a housing having a secondary flow passage outside the membranes, wherein the culture solution supplied from the tank is supplied to the primary flow passage and a permeated liquid containing the product permeate through the secondary flow passage to separate the cells and the product, and in the separation step, when a pressure difference between both ends of the primary flow passage during the feeding of the culture solution is P [Pa], and a viscosity of the culture solution is [Pa.Math.s], P/ is 4.910.sup.6 [1/s] or less.

Provided is a method for manufacturing a product, including a culture step of culturing cells, and a separation step of using a hollow fiber membrane filter which has a hollow fiber membrane having a primary flow passage extending and has a housing accommodating a plurality of the hollow fiber membranes, the hollow fiber membrane filter having a secondary flow passage, wherein the culture solution to the primary flow passage and a permeated liquid containing the product permeate through the secondary flow passage to separate the cells and the product, and in the separation step, when a pressure difference between both ends of the primary flow passage during the feeding of the culture solution is P [Pa], a membrane resistance of the hollow fiber membrane is r, and a flow passage resistance of the secondary flow passage is R, P/2(r+R) is 0.0005 mL/min or less.

Provided are a polyolefin filtration membrane and a preparation method therefor. The polyolefin filtration membrane includes a main body, both sides of the main body are provided with a first outer surface and a second outer surface, a non-directional tortuous pathway is formed in the main body, and a space between the first outer surface and the second outer surface is composed of continuous fibers. A PMI average pore size of the filtration membrane is 2-100 nm, the filtration membrane has an oxygen-to-carbon ratio in the range from 0.01 to 0.10. High-precision interception and removal of metal particles in a fluid to be filtered, micro-colloidal particles related to metal particles in photoresist, can be achieved through the cooperation between the interception effect of the PMI pore size on the metal particles and the adsorption effect of oxygen-containing functional groups of modified polyolefin molecular chains on the metal particles.