The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.

A monolithic separation membrane structure (100) comprises a base material layer (211) and a first filtration layer (212). The first filtration layer (212) contains an aggregate material having a principal component of alumina and an inorganic binder having a principal component of titania. The thickness of the first filtration layer (212) is less than 150 micrometers.

A monolithic separation membrane structure (100) comprises a base material layer (211) and a first filtration layer (212). The first filtration layer (212) contains an aggregate material having a principal component of alumina and an inorganic binder having a principal component of titania. The thickness of the first filtration layer (212) is less than 150 micrometers.

A gas separation membrane module includes a seal between a higher pressure gas and a lower pressure gas. The seal includes a compressible sealing member in between sealing surfaces. At least one of the sealing surfaces has corrosion-resistant cladding provided over either low alloy steel or high alloy steel.

The cladding reduce the possibility of a seal failure due to corrosion of low alloy or high alloy steel exposed to acid gases or condensed moisture containing acid gases dissolved therein while at the same not requiring that all surfaces of the membrane module exposed to acid gases be provided with cladding.

A gas separation membrane module includes a seal between a higher pressure gas and a lower pressure gas. The seal includes a compressible sealing member in between sealing surfaces. At least one of the sealing surfaces has corrosion-resistant cladding provided over either low alloy steel or high alloy steel. The cladding reduce the possibility of a seal failure due to corrosion of low alloy or high alloy steel exposed to acid gases or condensed moisture containing acid gases dissolved therein while at the same not requiring that all surfaces of the membrane module exposed to acid gases be provided with cladding.

The disclosed invention provides a hierarchically textured cement-based membrane exhibiting orthogonal wettability, specifically, superhydrophilic and underwater superoleophobic characteristics. In one embodiment, in situ formation of ettringite needles accompanied by embedding of solid impermeable structures such as cubes, cuboids, prisms, pyramids, platonic solids, torus, cone, cylinder, spheres or mixtures thereof, such as glass spheres, imbues micron- and nanoscale texturation to mesh membranes, such as stainless steel mesh membranes and provides for the separation of silt and oil from produced water at high flux rates (1600 L/h.Math.m2). Oil concentration can be reduced as low as 1 ppb with an overall separation efficiency of 99.7% in single-pass filtration.

The disclosed invention provides a hierarchically textured cement-based membrane exhibiting orthogonal wettability, specifically, superhydrophilic and underwater superoleophobic characteristics. In one embodiment, in situ formation of ettringite needles accompanied by embedding of solid impermeable structures such as cubes, cuboids, prisms, pyramids, platonic solids, torus, cone, cylinder, spheres or mixtures thereof, such as glass spheres, imbues micron- and nanoscale texturation to mesh membranes, such as stainless steel mesh membranes and provides for the separation of silt and oil from produced water at high flux rates (1600 L/h.Math.m2). Oil concentration can be reduced as low as 1 ppb with an overall separation efficiency of 99.7% in single-pass filtration.

A biofluid card assembly for isolating extracellular vesicles present in biofluids includes a biofluid card having a plurality of layers; and a fastener, the biofluid card includes a top lid with at least one opening configured to receive at least one biofluid sample including principal components and extracellular vesicles present therein, a biofluid separation membrane assembly disposed adjacent the top lid and configured to separate and direct the extracellular vesicles, at least one extracellular vesicle capture membrane having at least one extracellular vesicle capture agent formed thereon or embedded therein, each disposed below and adjacent the biofluid separation membrane assembly and configured to receive the extracellular vesicles, wherein each of the at least one extracellular vesicle capture agent includes a binding agent configured to bind to the extracellular vesicle having predetermined binding sites, and a bottom lid disposed adjacent the at least one extracellular vesicle capture membrane.

A recyclable waterproofing membrane has a bituminous binder with a recycled bituminous binder (RBB), optionally a fresh polymer and optionally fresh bitumen and a reinforcement. The bituminous binder has a rejuvenator selected from the group consisting of bio-based oils and/or mineral oils, such as plant-based, pine-oil based or vegetable-oil-based. The recycled bituminous binder is obtained by grinding and melting waterproofing membranes with bituminous binder, polymers and a reinforcement.

A recyclable waterproofing membrane has a bituminous binder with a recycled bituminous binder (RBB), optionally a fresh polymer and optionally fresh bitumen and a reinforcement. The bituminous binder has a rejuvenator selected from the group consisting of bio-based oils and/or mineral oils, such as plant-based, pine-oil based or vegetable-oil-based. The recycled bituminous binder is obtained by grinding and melting waterproofing membranes with bituminous binder, polymers and a reinforcement.