A macromolecule membrane structure (2) comprises a membrane (3) with water-channeling integral membrane proteins (IMPS) (1) and is coated, on a first surface, with a silica layer (4). The silica layer (4) stabilizes the macromolecule membrane structure (2) and the water-channeling IMPS (1) while maintaining the water-channeling function of the water-channeling IMPs (1). As a consequence of this stabilization, the macromolecule membrane structure (2) may be used in a filtration device (5) for various filtration operations, including water purification.

The present invention relates to an anti-fouling, semi-permeable membrane comprising a porous support layer, a thin film composite (TFC) layer formed on a surface of the support layer, and a cross-linked polyvinyl alcohol (PVA) layer formed on top of the TFC layer, wherein the cross-linked PVA layer is the reaction product of PVA and a cross-linking agent, said cross-linking agent being a polybasic acid comprising three or more acid groups or precursors thereof. The obtained membrane shows a high water flux and a low roughness suitable for an effective membrane notable for feed solution having a tendency of fouling the membrane.

The present invention relates to an anti-fouling, semi-permeable membrane comprising a porous support layer, a thin film composite (TFC) layer formed on a surface of the support layer, and a cross-linked polyvinyl alcohol (PVA) layer formed on top of the TFC layer, wherein the cross-linked PVA layer is the reaction product of PVA and a cross-linking agent, said cross-linking agent being a polybasic acid comprising three or more acid groups or precursors thereof. The obtained membrane shows a high water flux and a low roughness suitable for an effective membrane notable for feed solution having a tendency of fouling the membrane.

In a method for controlling translocation of a target polymer molecule through a nanopore, a clamp is reversibly bound at a site along the target polymer molecule length; the clamp and the target polymer molecule are disposed in an ionic solution that is in fluidic communication with a nanopore having an aperture diameter less than an outer diameter of the clamp. A constant translocation force is applied across the nanopore to induce travel of the target polymer molecule into the nanopore such that the reversibly bound clamp abuts the nanopore. A voltage pulse is applied across the nanopore that advances the target polymer molecule into the nanopore by one nucleotide, without either of chemical fuel and biochemical fuel provided to the clamp. The voltage pulse is repeatedly applied to cause a plurality of nucleotides to translocate through the nanopore. An indication of each nucleotide can be acquired during nucleotide translocation.

In a method for controlling translocation of a target polymer molecule through a nanopore, a clamp is reversibly bound at a site along the target polymer molecule length; the clamp and the target polymer molecule are disposed in an ionic solution that is in fluidic communication with a nanopore having an aperture diameter less than an outer diameter of the clamp. A constant translocation force is applied across the nanopore to induce travel of the target polymer molecule into the nanopore such that the reversibly bound clamp abuts the nanopore. A voltage pulse is applied across the nanopore that advances the target polymer molecule into the nanopore by one nucleotide, without either of chemical fuel and biochemical fuel provided to the clamp. The voltage pulse is repeatedly applied to cause a plurality of nucleotides to translocate through the nanopore. An indication of each nucleotide can be acquired during nucleotide translocation.

Disclosed are apparatus and methods for blood and other biological fluid purification using a membrane with cell containing vascular channel systems and filtration channel systems. Also disclosed are methods of making the apparatus as well as methods of making membranes.

Disclosed are apparatus and methods for blood and other biological fluid purification using a membrane with cell containing vascular channel systems and filtration channel systems. Also disclosed are methods of making the apparatus as well as methods of making membranes.

In a method for controlling translocation of a target polymer molecule through a nanopore, a clamp is reversibly bound at a site along the target polymer molecule length; the clamp and the target polymer molecule are disposed in an ionic solution that is in fluidic communication with a nanopore having an aperture diameter less than an outer diameter of the clamp. A constant translocation force is applied across the nanopore to induce travel of the target polymer molecule into the nanopore such that the reversibly bound clamp abuts the nanopore. A voltage pulse is applied across the nanopore that advances the target polymer molecule into the nanopore by one nucleotide, without either of chemical fuel and biochemical fuel provided to the clamp. The voltage pulse is repeatedly applied to cause a plurality of nucleotides to translocate through the nanopore. An indication of each nucleotide can be acquired during nucleotide translocation.

In a method for controlling translocation of a target polymer molecule through a nanopore, a clamp is reversibly bound at a site along the target polymer molecule length; the clamp and the target polymer molecule are disposed in an ionic solution that is in fluidic communication with a nanopore having an aperture diameter less than an outer diameter of the clamp. A constant translocation force is applied across the nanopore to induce travel of the target polymer molecule into the nanopore such that the reversibly bound clamp abuts the nanopore. A voltage pulse is applied across the nanopore that advances the target polymer molecule into the nanopore by one nucleotide, without either of chemical fuel and biochemical fuel provided to the clamp. The voltage pulse is repeatedly applied to cause a plurality of nucleotides to translocate through the nanopore. An indication of each nucleotide can be acquired during nucleotide translocation.

The present disclosure provides devices and methods for filtering a fluid. An example device can include a first end configured to be joined to a first segment of a pipe. The first end can include a first opening for receiving the fluid. The device can also include a second end configured to be joined to a second segment of the pipe. The second end can include a second opening for transmitting the fluid. A filtering segment can be disposed between the first end and the second end. The filtering segment can include a plurality of fiber filters oriented substantially perpendicular to a direction of flow of the fluid in the pipe. A fiber filter of the plurality of fiber filters can include a mycomaterial and a carrier material configured to provide nutrients to the mycomaterial.