The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

A method of manufacturing a base pipe for use in horizontal well completion, the method includes wrapping an inner shroud around a base pipe, the inner shroud is disconnected from the base pipe and forms seams along each edge of the inner shroud; fusing the seams together; allowing the inner shroud to cool, the inner shroud shrinks around the base pipe to form a friction lock between the base pipe and the inner shroud; laying a filter medium onto an outer shroud, wherein the filter medium is disconnected from the outer shroud, thereby creating a mesh filter medium/outer shroud sheet; wrapping filter medium/outer shroud sheet around the inner shroud, the filter medium is positioned between the inner shroud and outer shroud, thereby creating a second set of seams; and fusing the second set of seams together, thereby creating a second friction lock between the outer shroud and the inner shroud.

The present disclosure provides a water floating object treatment device for sewage treatment, including water pump, motor, round box, butt joint hole, liquid inlet pipe, electric adjusting valve, pneumatic controller, round frame, slurry pump, round tube, three-blade long plate, fan-shaped scraping plate, fine screw rod, guide sliding rod, wire brush bristle, assembling hole, fine-hole filter plate, strip hole, round plate, air cylinder, rubber round block, fan-shaped hole, threaded hole, and sliding hole. The present disclosure is reasonable in design, and function of filtering water in a treatment tank is realized. By replacing and installing the fine-hole filter plates with different sizes of holes, different degrees of filter effects are achieved. Meanwhile, the filtered water can be injected into corresponding treatment tank.

Support and drainage materials, filter including the materials, and methods of use are disclosed.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present disclosure provides a water floating object treatment device for sewage treatment, including water pump, motor, round box, butt joint hole, liquid inlet pipe, electric adjusting valve, pneumatic controller, round frame, slurry pump, round tube, three-blade long plate, fan-shaped scraping plate, fine screw rod, guide sliding rod, wire brush bristle, assembling hole, fine-hole filter plate, strip hole, round plate, air cylinder, rubber round block, fan-shaped hole, threaded hole, and sliding hole. The present disclosure is reasonable in design, and function of filtering water in a treatment tank is realized. By replacing and installing the fine-hole filter plates with different sizes of holes, different degrees of filter effects are achieved. Meanwhile, the filtered water can be injected into corresponding treatment tank.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

A filter arrangement includes: a chamber including a chamber inlet for unfiltered liquid, a chamber outlet for filtered liquid and a wall arranged to keep the unfiltered liquid and the filtered liquid separated, a filter element having a longitudinal extension arranged inside of the chamber, wherein the filter element includes a semi-permeable filtration wall including a folded filter arranged in a zig-zag configuration between an inner perforated cylinder and an outer perforated cylinder and an internally located back-washing arrangement. The back-washing arrangement includes: an elongated and hollow body having a longitudinal extension, which is substantially parallel to the longitudinal extension of the filter element, a mechanism configured to drive the body in a rotational and/or an axial movement, a plurality of nozzles arranged in fluid communication with a cavity inside of the body, the plurality of nozzles projecting laterally from the body such that a distal end of each of the plurality of nozzles is arranged in close proximity to the inner perforated cylinder and an outlet arranged in fluid communication with the cavity of the elongated and hollow body and wherein the plurality of nozzles are arranged parallel to each other along a straight line on the body such that they all project in the same direction.

A polymer melt candle filter having a support which comprises a hollow cylindrical body defined by a first end, a second end, and a cylinder wall having a first end portion and a second end portion. The first end portion includes a screw thread on its outside surface and optionally a hex fitting on its inside surface for receiving a hex wrench. The second end portion includes a plurality of holes in the cylinder wall. The area of the openings of the holes on the outside surface of the cylinder wall may be larger than the area of the openings of the corresponding holes on the inside surface of the cylinder wall. The shape of the openings of the holes on the outside surface may be non-circular. A polymer melt filter plate assembly which comprises (a) a support plate comprising a plurality of threaded holes; (b) at least one filter which is screwed into at least one of the threaded holes via the screw thread on the outside surface of the first end portion of the filter support; (c) a tie rod with (i) a first end extending through a hole in a cone insert and a cap located at the second end of the filter support, and (ii) a second end extending through a threaded hole in the support plate; and (d) a cross bar which is attached to the second end of the tie rod for holding the support plate inside a shaft of an apparatus for filtering a polymer melt.