The present invention relates to an evaporative membrane concentration device adapted to interface two liquid flow processes, such as two low or high resolution separation techniques or a low or high resolution separation technique and a liquid flow detection technique. For example, the two liquid flow processes may be a liquid chromatography technique and a liquid flow detection technique or a multi-dimensional separation technique, for example, two dimensional liquid chromatography (LCLC) or solvent extraction, such as liquid-liquid extraction or solid phase extraction, with a liquid chromatography technique (LLE or SPE-LC). Methods of using the device and separation and/or chromatographic methods using the device are also described.

The invention provides for methods and processes for producing, isolating, and purifying modified proteins. In particular, the invention provides for the production, isolation and purification of PEGylated recombinant methionyl human granulocyte colony stimulating factor used for therapeutic purposes.

An ion-exchange and ultrafiltration filter system having an exterior housing having an inlet and an outlet with an ultrafiltration membrane provided within the housing along a central axis about a central portion of the housing with an ion-exchange membrane provided within the housing between the ultrafiltration membrane and the housing. The ion-exchange and ultrafiltration filter system being capable of being configured so as to provide two-step filtration in a plurality of modes, either ion-exchange to ultrafiltration, or ultrafiltration to ion-exchange.

The present invention relates to a method for producing purified water comprising a step of passing water through an ultrafiltration means and a mixed bed ion exchanger comprising comprising beads having a pore size of 20-100 nm, wherein the ultrafiltration means is located upstream of said mixed bed ion exchanger, as well as to a module comprising an ultrafiltration means and a mixed bed ion exchanger and a water treatment system for producing ultrapure water comprising ultrafiltration means and a mixed bed ion exchanger.

The present invention relates to a method for producing purified water comprising a step of passing water through an ultrafiltration means and a mixed bed ion exchanger comprising comprising beads having a pore size of 20-100 nm, wherein the ultrafiltration means is located upstream of said mixed bed ion exchanger, as well as to a module comprising an ultrafiltration means and a mixed bed ion exchanger and a water treatment system for producing ultrapure water comprising ultrafiltration means and a mixed bed ion exchanger.

A process to remove transition metals from waste water. The process includes the steps of passing waste water to a first pH resin bed, monitoring the effluent from the first resin bed, and adjusting pH to greater than 4. The effluent is passed to a first stage liquid tank and to a first brackish water membrane to filter out complex metals. Rejected effluent from the first brackish water membrane is passed to a second stage liquid tank and thereafter to a second brackish water membrane. The permeate from the second brackish water membrane is passed back to the first stage liquid tank. The rejected effluent from the second brackish water membrane is heated and evaporated. The evaporated effluent is condensed so that metal crystals are gathered for disposal. The permeate through the first brackish membrane is passed to an EDTA resin bed to sequester metal ions. The pH of the discharge from the second pH resin bed is adjusted to between 7 and 11.

The ultrapure-water production system includes an auxiliary treatment apparatus and a dead-end filtration apparatus. The auxiliary treatment apparatus treats water such that the number of microparticles present in water treated by the auxiliary treatment apparatus is 800 to 1200 particles/mL. The dead-end filtration apparatus includes a filtration membrane that is a microfiltration membrane having pores formed in the surface of the membrane at an opening ratio of 50% to 90% with a size of 0.05 to 1 m, and having a thickness of 0.1 to 1 mm, or an ultrafiltration membrane having pores formed in the surface of the membrane with a size of 0.005 to 0.05 m, the number of the pores being 1E13 to 1E15 pores/m.sup.2, the ultrafiltration membrane having a thickness of 0.1 to 1 mm and a transmembrane pressure of 0.02 to 0.10 MPa at a permeation flux of 10 m.sup.3/m.sup.2/d.

An object of the present invention is to provide a chemical liquid purification method which makes it possible to obtain a chemical liquid having excellent defect inhibition performance. The chemical liquid purification method according to an embodiment of the present invention is a chemical liquid purification method including obtaining a chemical liquid by filtering a substance to be purified containing an organic solvent by using two or more kinds of filters having different pore sizes, in which a supply pressure P.sub.1 of the substance to be purified supplied to a filter F.sub.max having a maximum pore size X.sub.1 among the two or more kinds of filters and a supply pressure P.sub.2 of the substance to be purified supplied to a filter F.sub.min having a minimum pore size X.sub.2 among the two or more kinds of filters satisfy P.sub.1>P.sub.2.

The present invention provides a method for extracting -polylysine (-PL) and its hydrochloride salt from fermentation broth, which belongs to the field of bio-separation engineering. -PL and its hydrochloride salt are produced from fermentation broth through sequential solid-liquid separation, ultrafiltration, two-stage ion exchange, nanofiltration, evaporation concentration and drying techniques. Technologies of membrane filtration and two-stage ion exchange are applied to the preparation of -PL and its hydrochloride salt in the present invention, and the invention are characterized by reduced cost, improved automation, and increased product yield and purity, and the method of the present invention would be more suitable for industrial production.

The present invention provides a method for extracting -polylysine (-PL) and its hydrochloride salt from fermentation broth, which belongs to the field of bio-separation engineering. -PL and its hydrochloride salt are produced from fermentation broth through sequential solid-liquid separation, ultrafiltration, two-stage ion exchange, nanofiltration, evaporation concentration and drying techniques. Technologies of membrane filtration and two-stage ion exchange are applied to the preparation of -PL and its hydrochloride salt in the present invention, and the invention are characterized by reduced cost, improved automation, and increased product yield and purity, and the method of the present invention would be more suitable for industrial production.