A working medium includes a first amine compound and a second amine compound. The first amine compound is a heterocyclic tertiary amine compound including a carbon atom, a nitrogen atom and a hydrogen atom, and in which a ratio (C/N ratio) of a carbon atom number to a nitrogen atom number included in one molecule is from 7 to 9. The second amine compound is a heterocyclic tertiary amine compound including a carbon atom, a nitrogen atom and a hydrogen atom. and in which a ratio (C/N ratio) of a carbon atom number to a nitrogen atom number included in one molecule is in a range of 5 or more to less than 7.

The present disclosure provides a system for refining long chain dicarboxylic acid, comprising: a first membrane filtration unit, for a first membrane filtration of a long chain dicarboxylic acid fermentation broth or a treated liquid therefrom; a first decolorization unit, for carrying out a first decolorization treatment to the filtrate obtained after the membrane filtration; a first acidification/crystallization unit, for carrying out a first acidification/crystallization of a filtrate obtained after the membrane filtration to give a solid-liquid mixture; a first separation unit, for a solid-liquid separation of the solid-liquid mixture; a drying unit, for drying the solid separated by the separation unit to give a first solid. By using the refining system according to the present disclosure, the purity of the obtained product is high, and the disadvantages such as poor quality of the product obtained by crystallization from a solvent and environment pollution caused by a solvent can be overcome.

The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.

The present invention discloses a microencapsulation method for improving stability of anthocyanin, a product therefrom and use thereof. A preparation method of anthocyanin microcapsules includes: (1) taking sodium alginate as a wall material, adding sodium alginate and calcium carbonate into water, and swelling for 1-2 h to obtain a wall material gel system; (2) taking anthocyanin prepared by a special process as a core material, and fully and uniformly mixing the wall material gel system with an anthocyanin solution to obtain a water phase; (3) mixing Span80 and vegetable oil to obtain an oil phase, mixing the water phase with the oil phase, and magnetically stirring for emulsifying to obtain a W/O emulsion; and (4) adjusting the pH of the W/O emulsion to be acidic, mixing the W/O emulsion with a salt buffer solution, standing for 1-2 h, and then separating the oil phase and the water phase.

The present invention relates in general to the field of water kiosks, and more specifically, to an emergency water filtration kiosk and method of delivering clean and safe water using the emergency water filtration kiosk. One aspect of the emergency water filtration kiosk and method of use may include a four-stage water filtration system to better purify, clean, and improve the taste of water. The emergency water filtration kiosk and method of use may further include a turn-key variable pump system that is configured to supply water to the emergency water filtration kiosk via three alternative power sources. The purpose of the invention is to provide a self-contained and rapidly deployable emergency response water filtration kiosk and method of use that delivers clean and safe drinking water to people in need after a natural disaster. An additional purpose of the invention is to provide an emergency water filtration kiosk and method of use that offers a wide variety of on-board integrated pumping solutions to supply water to the filtration kiosk under any power condition.

The present invention is related a method of purifying a heterologous protein from an egg white. In some embodiments, the disclosure is directed to a method of purifying a heterologous protein from an egg white comprising the heterologous protein, the method comprising, (a) adjusting the pH of the egg white to a pH of 5.8 to 6.5 to form a pH-adjusted egg white; (b) filtering the pH-adjusted egg white of (a) and collecting a first filtrate; (c) subjecting the first filtrate of (b) to a hydrophobic interaction chromatography matrix, and collecting a first eluate comprising the heterologous protein; (d) adjusting the pH of the first eluate of (c) to a pH of 5.0 to 5.6 to form a pH-adjusted eluate; (e) filtering the pH-adjusted eluate to obtain a second filtrate; (f) adjusting the pH of the second filtrate to a pH of 3.0 to 4.0 to form a pH-adjusted second filtrate; (g) neutralizing the pH-adjusted second filtrate of (f) to a pH of 5.0 to 8.0 to form a neutralized solution; (h) subjecting the neutralized solution to a cation exchange chromatography matrix and collecting a second eluate comprising the heterologous protein.

A system for generating sterile water is provided, including: a liquid holding container having thereon a container outlet; a filtration device including therein a low-pressure-differential pathogen filtration portion, wherein the filtration device has a filtration inlet and a filtration outlet; and a gas tube; wherein the container outlet is connected to the filtration inlet; wherein the filtration inlet has a gas hole with one end connected to the gas hole and the other end having a buoy; wherein the sterile water is substantially pathogen-free.

A method for preparing battery grade and high purity grade lithium hydroxide and lithium carbonate from high-impurity lithium sources includes steps for preparation of a refined lithium salt solution, preparation of battery grade lithium hydroxide, preparation of high purity grade lithium hydroxide, preparation of high purity grade lithium carbonate and preparation of battery grade lithium carbonate. The system to carry out the preparation includes a refined lithium salt solution preparation subsystem, a battery grade lithium hydroxide preparation subsystem, a high purity grade lithium hydroxide preparation subsystem, a high purity grade lithium carbonate preparation subsystem and a battery grade lithium carbonate preparation subsystem arranged in turn according to production sequence. A combination of physical and chemical treatment methods are used to treat the high-impurity lithium sources having variations in lithium contents, impurity categories, and impurity contents.

A device for purifying drinking water in multiple stages by combining orthogonal purification technologies in one module is described, where the device comprises a housing, a water inlet opening, a water outlet opening, a hollow cylinder which is filled with activated carbon, and a hollow cylinder with a semipermeable wall, wherein the hollow cylinder contains a chelating bactericidal gel or a chelating and bactericidal gel for removing heavy metals or bacteria or for removing heavy metals and bacteria.

The present invention is directed to soy protein products, very low in, or free of, beany flavour notes and useful for the fortification of food and beverage products and prepared without the use of salt in the process. The soy protein products of the present invention are obtained by extracting soy protein source with water to form an aqueous soy protein solution, at least partially separating the aqueous soy protein solution from residual soy protein source, adjusting the pH of the aqueous soy protein solution to a pH of about 1.5 to about 3.6 to solubilize at least a portion of the protein and form an acidified soy protein solution then separating the acidified soy protein solution from the acid insoluble solid material. The acidified soy protein solution may be dried following optional concentration and diafiltration to form a soy protein product, which may be an isolate. The acid insoluble solid material may be washed with acidified water and then dried to form another soy protein product. These products may be dried at the acidic pH at which they were prepared or may be adjusted in pH before drying.