Disclosed herein are aspects and embodiments of an apparatus for providing gas slugs in a liquid. In one example, the apparatus comprises a housing defining a chamber, a bell tube including a wall and an open upper end disposed within the chamber, a riser tube including a wall, an open lower end, and a flared open upper end disposed within the bell tube, and a conduit defined between the wall of the bell tube and the wall of the riser tube and in fluid communication with an internal volume of the riser tube.

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.

Provided is a fresh water generation system which filters biotreated water produced by microbiologically treating waste water via a primary semi-permeable membrane (8), and then mixes concentrated water rejected by the filtration with seawater, thereby to filter the mixed water via a secondary semi-permeable membrane (14). This system enables frequencies of chemical cleaning and replacement of the secondary semi-permeable membrane to be reduced. The fresh water generation system comprises: a primary semi-permeable membrane apparatus (9) which separates microorganism treating waste water into permeated water and concentrated water; a secondary semi-permeable membrane apparatus (13) which filters mixed water prepared by mixing the concentrated water into seawater. In the fresh water generation system, the primary semi-permeable membrane (8) has an equal to or a higher microorganism adhesive property than the secondary semi-permeable membrane (14).

Provided is a fresh water generation system which filters biotreated water produced by microbiologically treating waste water via a primary semi-permeable membrane (8), and then mixes concentrated water rejected by the filtration with seawater, thereby to filter the mixed water via a secondary semi-permeable membrane (14). This system enables frequencies of chemical cleaning and replacement of the secondary semi-permeable membrane to be reduced. The fresh water generation system comprises: a primary semi-permeable membrane apparatus (9) which separates microorganism treating waste water into permeated water and concentrated water; a secondary semi-permeable membrane apparatus (13) which filters mixed water prepared by mixing the concentrated water into seawater. In the fresh water generation system, the primary semi-permeable membrane (8) has an equal to or a higher microorganism adhesive property than the secondary semi-permeable membrane (14).

The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.

A perfusion bioprocessing system (10) includes a bioreactor (12) and a recirculation flow path (14) provided with at least in one first feed flow control device (46) and at least one second feed control device (48). The perfusion bioprocessing system (10) further includes a first tangential flow filter (16) coupled to the bioreactor (12) via the recirculation flow path (14) and a second tangential flow filter (18) coupled to the bioreactor (12) via the recirculation flow path (14). The first tangential flow filter and the second tangential flow filter are coupled to a permeate flow path and a retentate flow path. Additionally, the perfusion bioprocessing system (10) includes a control unit (90) coupled to the at least one first feed flow control device (38) and the at least one second feed control device (40).

A cell culture bioreactor has perfusion membranes and gas transfer membranes or a gas phase in an extra-membrane space in contact with a film on the perfusion membranes. Gas transfer membranes may travel through the perfusion membranes or through the extra-membrane space. Examples with hollow fiber and flat sheet membranes are shown. One or more of the membranes optionally has a responsive surface, for example a thermo-responsive surface. In some examples, membranes are located in X-Y planes while the length of the reactor extends in a Z-direction.

Disclosed herein are systems, devices, and methods for flowing fluid for radially expanded particle distribution within a laminar flow. In some variations, a system for cultivating tissue may comprise a bioreactor comprising an inlet, a substrate arranged in the bioreactor, and a diffusion module configured to transfer fluid from the inlet to the substrate. The diffusion module may comprise a porous material having at least one tortuous conduit extending between a first surface of the porous material and a second surface of the porous material.

The present disclosure provides a cell separation apparatus for a bioreactor. The cell separation apparatus may be disposed outside the bioreactor and in fluid connection with the bioreactor, the cell separation apparatus may be in a shape of a box body, the cell separation apparatus may include a liquid buffer device including a first liquid cavity disposed in the box body; a filter device including a filter channel and a filter membrane disposed in the box body, the filter membrane may be disposed above the filter channel; and a first liquid channel may be configured in the box body to facilitate a fluid communication between the first liquid cavity and the filter channel. A power system for filtering and microfluidic channels are integrated in the cell separation apparatus that is of a box shape, thereby reducing the volume and production cost thereof.