The present disclosure relates generally to alternating tangential flow (ATF) perfusion pumping methods, including novel methods for generating positive and negative pressure at the point of use, and more particularly, to apparatuses, systems and methods for use of the same.

A multiple-loop tangential flow filtration apparatus for concentrating fluids is described herein. The apparatus comprises a plurality of tube loops for receiving fluid therethrough, each tube loop comprising a respective filter, and a common feed pump for driving the fluid across each respective filter. The plurality of tube loops are coupled to the common pump via a common feed line.

The present disclosure provides a cell separation apparatus for a bioreactor. The cell separation apparatus may be disposed outside the bioreactor and in fluid connection with the bioreactor, the cell separation apparatus may be in a shape of a box body, the cell separation apparatus may include a liquid buffer device including a first liquid cavity disposed in the box body; a filter device including a filter channel and a filter membrane disposed in the box body, the filter membrane may be disposed above the filter channel; and a first liquid channel may be configured in the box body to facilitate a fluid communication between the first liquid cavity and the filter channel. A power system for filtering and microfluidic channels are integrated in the cell separation apparatus that is of a box shape, thereby reducing the volume and production cost thereof.

An arrangement for providing sterile water for injection purposes is described. A device for heating drinking water above the boiling point, a device for maintaining a chamber inner pressure which lies below the atmospheric pressure, and an electronic controller are provided, and the chamber is equipped with at least one membrane which is impermeable for liquids and a film or plate at a distance from the membrane, wherein steam which is permeated through the membrane is condensed on the film of plate. The membrane and the film or plate form a module, and the condensed water can be removed from the chamber via an outlet as sterile water for injection purposes.

A water filter unit for purifying water fed to the water filter unit. The unit includes a containment defined by a wall section, first and second end sections, wherein the wall section is attached to the first end section and the second end section; a water inlet arranged in the first or second end section through which the water is fed into the containment; a filter membrane arranged in the containment such that at least part of the water is fed through the filter membrane; and a first water outlet for filtered water from the membrane. The unit further includes an injector pump arranged to create an increased flow velocity trough the injector pump of the water fed to the filter membrane, and the injector pump is furthermore arranged to receive unfiltered water not fed through the membrane and recirculate the unfiltered water within the containment.

An apparatus and a method use permeate to flush a reverse osmosis filter membrane in the same flow direction as when fluid is being filtered through the membrane. The apparatus includes a housing having a membrane positioned therein. The membrane has an interior surface and an exterior surface. The housing has an inlet port located outward of the exterior surface of the membrane and first and second outlet ports. The first outlet port is located outward of the exterior surface of the membrane and the second outlet port is located inward of the interior surface of the membrane. A first pump is used to route pressurized, untreated fluid from a fluid source through the membrane to produce permeate. A second pump is used to route permeate to a desired function while also routing some permeate at low pressure to the housing to flush the membrane.

Various reverse osmosis (RO) filter systems are disclosed herein. The RO filter systems can include one or more RO filters connected to and in fluid communication with an end cap filter component and/or a fluid turbine component. Some RO filter systems can further include a filter component connected to and in fluid communication with the one or more RO filters and/or a fluid turbine component. Additional RO filter systems can further include a pre-filter component connected to the filter component.

A degasser for at least partially degassing a gas-containing liquid, in particular for a sample separation device, includes a liquid accommodation volume for accommodating the gas-containing liquid during degassing, a negative pressure chamber in which a negative pressure, compared to the liquid accommodation volume, is to be generated, a gas permeable membrane separating the liquid accommodation volume from the negative pressure chamber and arranged so that ultrasound forces at least part of gas of the gas-containing liquid to move through the membrane by a combination of the negative pressure and the ultrasound, and an ultrasound source including an electroactive material and configured for generating ultrasound for actuating the gas-containing liquid and/or the gas permeable membrane.

A large scale water desalination process for producing at least 100,000 m.sup.3/day of product water. Feed water is passed through a high pressure pump driven by at least one steam turbine capable of producing at least 1 MW of energy, the pressurized feed water passing through at least one reverse osmosis membrane to provide a residual brine stream and a product water. A start-up step slowly increases pressure in the membrane at a maximum rate of 12 psi (8.3 Newtons/cm.sup.2; 0.08 MPa) per second by rotation of the turbine driven high pressure pump at a maximum rate of 30 RPM to slowly increase pressure on the membrane to a predetermined operational pressure and controlling the operational pressure following the start-up step by rotation of the high pressure pump between 500 RPM and 5000 RPM dependent on the pressure applied by the steam turbine.

An apparatus for producing ultrapure water: first ultrafiltration membrane that is connected to point of use and that supplies ultrapure water to point of use; first concentrated water return line that returns concentrated water of first ultrafiltration membrane to an upstream side of first ultrafiltration membrane; pressure gauge that measures pressure at an outlet of first ultrafiltration membrane; and means for adjusting flow rate of the concentrated water (first valve). Means for adjusting the flow rate of the concentrated water can be operated such that when the flow rate of the concentrated water is changed, a change in the pressure at the outlet of first ultrafiltration membrane that is measured by pressure gauge is kept within a predetermined range.