A bioreactor having a filter unit and a method for treating a cell broth. The filter unit has a supply channel (2), a first filter medium (4), a retentate channel (1), a second filter medium (5) and a permeate channel (3), arranged so that the first filter medium delimits the supply channel and the retentate channel from one another; and the second filter medium delimits the retentate channel and the permeate channel from one another. The supply channel is connected to an inlet for a supply medium; the retentate channel is connected to an inlet for a cell broth and to an outlet for the cell broth; the permeate channel is connected to an outlet for a permeate; and the interior of the bioreactor is connected to the inlet for the cell broth and to the outlet for the cell broth.

A purification system for a colloidal dispersion containing an unwanted dissolved substance in the dispersing liquid phase is described which includes a back-pressure regulating feature, disposed in a first conduit and downstream of a semi-permeable filter element. This is used to force a portion of the unpurified dispersing liquid across the filter element and into a second conduit. The second conduit is associated with an adsorptive element that serves to remove the unwanted dissolved substance from the filtered portion of the dispersing liquid. A mixing chamber is disposed downstream of the back-pressure regulating feature whereby the purified dispersing liquid portion from the second conduit rejoins the bulk flow of the colloidal dispersion passing through the first conduit. In a preferred embodiment, hollow fibers are used which serve as the back-pressure regulating feature, the adsorptive element, and the mixing chamber before being discharged at the outlet of the device.

Aspects of the present disclosure relate to filtration systems and methods for production of biologically-produced products, which may include pharmaceutical and/or protein products. Certain biomanufacturing systems described herein comprise a bioreactor (e.g., a perfusion bioreactor, a chemostat) and a filter probe comprising a filter bundle comprising a plurality of hollow fibers (e.g., longitudinally aligned hollow fibers). According to some embodiments, a center-to-center distance between any two hollow fibers within the fiber bundle at one or more points along a length of the fiber bundle is relatively large (e.g., greater than or equal to an average outer diameter of the hollow fibers of the fiber bundle, greater than or equal to 1.1 times a minimum diameter of the two hollow fibers). In some embodiments, the hollow fibers within the fiber bundle are arranged in an array (e.g., a hexagonal, linear, annular, or square array).

In one aspect, a modular filter assembly for filtering fluid that flows in a flow direction therethrough is disclosed. The assembly includes a first housing section having a first filter element; a second housing section having a second filter element; and a connector disposed between the first and second housing sections. A housing includes the first housing section, the connector, and the second housing section. The flow direction is through the first and second filter elements in series.

A membrane apparatus including a housing, a forward osmosis membrane dividing an internal space of the housing into an inlet region and a mixing region, and a pervaporation membrane dividing the internal space of the housing into the mixing region and a discharge region. The forward osmosis membrane separates a preliminary filtration liquid from an inlet liquid and provides the separated preliminary filtration liquid to the mixing region, the preliminary filtration liquid is mixed with a forward osmosis draw solution to make a mixed solution, the pervaporation membrane separates a final filtration liquid from the mixed solution and provides the separated final filtration liquid to the discharge region, the final filtration liquid is vaporized in the discharge region to make vapor, and an amount of the vapor is adjusted by at least one of a temperature of the mixed solution and a degree of vacuum of the discharge region.

A microfluidic tissue dissociation and filtration device simultaneously filters large tissue fragments and dissociates smaller aggregates into single cells, thereby improving single cell yield and purity. The device includes an inlet coupled to a first microfluidic channel at an upstream location and a first outlet at a downstream location. A first filter membrane is interposed between the first microfluidic channel and a second microfluidic channel, wherein the second microfluidic channel is in fluidic communication with the first microfluidic channel via the first filter membrane. The first filter membrane operates under a tangential flow format. A second outlet is coupled to a downstream location of the second microfluidic channel and includes a second filter membrane interposed between the second outlet and the second microfluidic channel. The dual membrane device increased single cell numbers by at least 3-fold for different tissue types.

The disclosure provides a hollow fiber liquid filter device provided with exhaust fiber membranes. The hollow fiber liquid filter device comprises a shell, a liquid inlet end, a liquid outlet end, a melt-blown fiber filter element, a hollow fiber liquid filtering part and a hollow fiber gas filtering part, wherein the liquid inlet end and the liquid outlet end are respectively connected to the upper end and the lower end of the shell, the melt-blown fiber filter element is arranged on the inner side of the shell, the hollow fiber liquid filtering part and the hollow fiber gas filtering part are respectively arranged on the inner side of the melt-blown fiber filter element, a liquid inlet is formed in the liquid inlet end, and ,a liquid outlet is formed in the liquid outlet end.

A filtering device is for obtaining a chemical liquid by purifying a liquid to be purified, and the filtering device has an inlet portion, an outlet portion, a filter A, at least one filter B different from the filter A, and a flow path which includes the filter A and the filter B arranged in series and extends from the inlet portion to the outlet portion, in which the filter A is a porous membrane containing a polyimide-based resin.

A fiber bundle cartridge for a fiber membrane degassing system includes an inner sleeve including one or more perforations and a fiber bundle positioned radially outward from the inner sleeve. The fiber bundle has an annular shape defining a central bundle axis. The perforations of the inner sleeve define a plurality of inlets and/or outlets facing radially with respect to the central bundle axis. A fiber membrane degassing system includes a housing defining a cylindrical volume having at least one inlet and at least one outlet. The system includes at least one fiber within the cylindrical volume, where fluid flowing through the cylindrical volume from the at least one inlet to the at least one outlet flows perpendicular to a longitudinal dimension of the fiber. A method of degassing a liquid includes directing a liquid volume through a fiber bundle in a direction radial to a longitudinally extending bundle axis.

This invention describes a method and a device for efficient isolation of extracellular vesicles from animal and human biological fluids, as well as from culture fluid using equipment of standard diagnostic laboratories, that is, without the use of ultracentrifugation. These method and device can be applied for the diagnosis of various human diseases, as well as for therapeutic purposes, if the purified vesicles are used as an agent for drug delivery to the cells of the body. The device for the purification of extracellular vesicles contains at least two membrane filters: the first filter containing a membrane with pore sizes in the range from 400 to 600 nm, connected to the second filter containing a membrane with pores in the range from 95 to 200 nm. At the same time, the membranes of these filters are made of materials that practically do not bind biological polymers.