A lipid nanoparticles manufacturing chip includes a mixer unit for forming a mixed solution by mixing a first raw material containing an active ingredient and a second raw material containing a lipid, a dilution unit that is connected to the mixer unit and dilutes the mixed solution using a diluent solution to make a diluted mixed solution, and a concentration unit connected to the dilution unit and for obtaining a concentrated solution by concentrating lipid nanoparticles (LNP) from the diluted mixed solution.

The present disclosure relates to apparatuses for producing nanoparticles from a material in a working liquid and optionally to produce hydrogen, and to top-down methods for producing nanoparticles and optionally hydrogen using such apparatuses. A core of such an apparatus can include a first disc and a second disc including holes arranged in concentric rings and channel portions between those rings. The first disc and the second disc are arranged such that the channels of each disc face the rings of the other disc.

The present disclosure relates to systems and methods for producing nanoparticles of a material in a working liquid. The apparatus can include a core for accelerating the working liquid and the material, wherein the core includes: a first cylinder including: a radially outer surface and a radially inner surface; and a plurality of first through holes extending from the radially inner surface to the radially outer surface of the first cylinder; a second cylinder including: a radially outer surface and a radially inner surface; and a plurality of second through holes extending from the radially inner surface to the radially outer surface of the second cylinder; wherein the second cylinder radially surrounds the first cylinder; wherein each of the first through holes and the second through holes have a smaller cross-section at the radially inner surface than at the radially outer surface.

Methods for mixing fluids including etanercept include providing a tank having an impeller with a 10 inch blade diameter disposed therein, filling a tank with a liquid composition comprising etanercept and one or more buffers to a level within the tank to cover the impeller, and operating the impeller to mix the liquid composition to a homogeneous state while maintaining a maximum shear stress imparted on the mixture of fluids at 125,000 s.sup.1 or below.

A reactor system includes a reactor vessel configured to contain a process fluid, and a sparger assembly that operably coupled to the reactor vessel and configured to supply a mixture of a gas and a recirculated process fluid to the reactor vessel. The sparger assembly includes a plurality of sparger chambers. Each sparger chamber includes a process fluid conduit fluidly coupled to a process fluid return of the reactor vessel via a process fluid inlet, wherein the process fluid inlet has a first block and bleed valve assembly. Each sparger chamber includes a sparger conduit fluidly coupled to the process fluid conduit and a sparger disposed within the sparger conduit and fluidly coupled to a gas source via a gas inlet. Each sparger chamber also includes a process fluid-gas mixture outlet that fluidly couples the sparger conduit to a sparger outlet of the reactor vessel.