This invention describes a design for a multiwell plate that contains integrated pumps that are used to stir each well of the plate. The device employs microfluidic logic technology to drive each peristaltic pump. This enables the plates to run autonomously, requiring only a static vacuum supply for power. The devices are entirely constructed out of low-cost polymers, with no electronics, and yet contains simple digital logic circuits to control the pumps. A stack of these plates may be run continuously in a standard cell culture incubator, allowing high-throughput culture of organoids.

A dosing and mixing arrangement includes a mixing tube having a constant diameter along its length. At least a first portion of the mixing tube includes a plurality of apertures. The arrangement also includes a swirl structure for causing exhaust flow to swirl outside of the first portion of the mixing tube in one direction along a flow path that extends at least 270 degrees around a central axis of the mixing tube. The arrangement is configured such that the exhaust enters an interior of the mixing tube through the apertures as the exhaust swirls along the flow path. The exhaust entering the interior of the mixing tube through the apertures has a tangential component that causes the exhaust to swirl around the central axis within the interior of the mixing tube. The arrangement also includes a doser for dispensing a reactant into the interior of the mixing tube.

The present invention provides a set of ozone water manufacturing device which can greatly reduce an unpleasant smell of ozone gas when ozone water is used for sterilizing hands and feet, and can save power consumption of a pump which operates for preventing stagnation of a water stream in an electrolytic bath. The ozone water manufacturing device includes an electrolytic bath and a mixer which atomizes air bubbles of the ozone gas in the ozone water flowing out of the electrolytic bath. Each of the electrode assemblies in an electrode unit within the electrolytic bath is inclined at the predetermined same angle in a vertical direction. The mixer includes a mixing case bottom and a mixing case main body. A vortex flow generating plate generates a violent water stream from the water stream within the mixing case main body.

An apparatus, system and method to reduce the contaminate concentration of effluent before discharge is provided utilizing discharge pipes, inlets and outlets. The apparatus comprises a central bore having an internal diameter suitable for a fluid flow, the fluid flow moves inside the central bore through the apparatus, and at least one outlet, the fluid flow exits the apparatus through the at least one outlet, a plurality of inlets for flowing additional fluid to the central bore, and the inlets mix the fluid flow with the additional fluid from the plurality of inlets. The apparatus can further mix the effluent though additional mixing devices and additional devices can be used to recapture energy such as, hydroelectric power from the fluid flow. A method reduces the effluent concentration by mixing for example, by creating a helical flow in the central bore.

A method of reconstituting a powdered cell culture media includes providing a mixing apparatus and providing a powdered cell culture media to the fluid chamber of the mixing apparatus, prior to introducing fluid to the chamber. The method further includes introducing fluid to the chamber through an influent port, wherein the influent port is tangentially oriented relative to an inner wall of the fluid chamber to thereby cause the fluid to follow the wall of the fluid chamber in a circular motion, creating a vortex flow in the fluid chamber by introducing fluid at a sufficient flow rate, and enhancing the vortex flow with a geometric fluid flow aid by further channeling the water around the wall of the fluid chamber, around the geometric flow aid. The method further includes continuing to introduce fluid to the chamber and collecting reconstituted fluid that exits the chamber through the effluent port.

The present invention relates to a mixing device (1), more in particular a mixing device (1) for the production of nanoparticles from the mixing of at least two fluids, wherein the mixing device (1) comprises a mixing chamber (2), two inlet conduits (3, 4) and an outlet (5).

A system for preparing a polymeric mixture includes: a containment device configured to distribute dry polymeric materials; a receiving chamber in fluid communication with the containment device; a wetting bowl; a dispersing channel; and a mixing chamber connected to the dispersing channel. A method of preparing a polymeric mixture includes distributing water and dry polymeric materials through the various components of the system and mixing the materials with the mechanical mixing device.

An automated method includes providing a dry ingredient to be reconstituted into a liquid bioprocess solution and controlling, by a processing circuit, all automated system including at least one mixing chamber, an array of tubing for fluid flow within the system, and a plurality of valves provided within the tubing, to automatically prepare the liquid bioprocess solution from the dry ingredient. Controlling the auto system may include performing a series of sequential mixing steps, the series of sequential mixing steps causing the preparation of the liquid bioprocess solution. The method mixing include taking one or more measurements during the preparation of the liquid bioprocess solution, wherein each step is triggered by at least one of a measurement decreasing below, equaling, or exceeding a measurement threshold. Each step may also include opening or closing, by the processing circuit, at least one of the plurality of valves.

A mixer for a plural component spray gun is presented. The mixer has a mixer body comprising a mixing chamber with an outlet. The mixer also has a first fluid component inlet, coupled to a first fluid conduit, configured to introduce a first fluid component into the mixing chamber. The mixer also has a second fluid component inlet, coupled to a second fluid conduit, configured to introduce a second fluid component into the mixing chamber. The first and second fluid component inlets are offset with respect to a centerline of the mixing chamber and positioned such that a first fluid flow from the first inlet is directed toward the outlet, and a second fluid flow from the second inlet is directed toward the outlet.

A mixing apparatus for reconstituting a powdered cell culture media. The apparatus includes at least one fluid chamber, an influent port at a lower portion of the fluid chamber. The apparatus further includes a geometric fluid flow aid positioned in the fluid chamber and an effluent port at the top of the fluid chamber. A powdered cell culture media is provided in the fluid to be mixed with a fluid provided by the influent port. The effluent port is configured to allow reconstituted media to exit the fluid chamber.