Disclosed is a method for managing the operation of an apparatus for injecting oxygen into a purification basin. The oxygen notably being used by the biomass present in the purification basin to consume the pollution present in an effluent feedstock contained in the basin. The method comprises varying a rotational speed of the shaft by using a frequency variator, wherein an applied variation in speed is between plus 15% and minus 15% of the nominal speed of the shaft.

A method of pumping fluid out of a bioprocess vessel includes providing a bioprocess vessel defining an interior volume and having a bottom surface containing an aperture or port therein for the passage of fluid and a pump head integrally formed with or bonded to the bottom surface of the bioprocess vessel or secured to the port, wherein the pump head further comprises an inlet and an outlet with the inlet in fluid communication with the interior volume, the pump head further including a plurality of diaphragms, each diaphragm associated with a check-valve, wherein sequential actuation of the plurality of diaphragms pumps fluid from the interior volume of the bioprocess vessel into the inlet and out of the outlet. The plurality of diaphragms are actuated with a motor secured to the pump head. In other embodiments, the pump head is secured to the bioprocess vessel with fasteners.

A method of pumping fluid out of a bioprocess vessel includes providing a bioprocess vessel defining an interior volume and having a bottom surface containing an aperture or port therein for the passage of fluid and a pump head integrally formed with or bonded to the bottom surface of the bioprocess vessel or secured to the port, wherein the pump head further comprises an inlet and an outlet with the inlet in fluid communication with the interior volume, the pump head further including a plurality of diaphragms, each diaphragm associated with a check-valve, wherein sequential actuation of the plurality of diaphragms pumps fluid from the interior volume of the bioprocess vessel into the inlet and out of the outlet. The plurality of diaphragms are actuated with a motor secured to the pump head. In other embodiments, the pump head is secured to the bioprocess vessel with fasteners.

A laundry machine according to an embodiment of the present invention, comprises: a cabinet; a tub rotatably disposed within the cabinet; and a detergent supply device provided in the cabinet for supplying detergent to the tub, wherein the detergent supply device comprises: a housing fixedly mounted to the cabinet and connected to a water supply device; a drawer withdrawably mounted to the housing, and including a receiving portion recessed to accommodate detergent and a rotating boss extending upward from a bottom of the receiving portion; a mixing member rotatably disposed in the receiving portion to mix the detergent inside the receiving portion; and a flow path member that is connected to the housing at a position above the drawer and forms a flow path for supplying water from the water supply device to the drawer, wherein the mixing member includes: a rotation axis, and a plurality of blades extending radially from an outer surface of the rotation axis, wherein the flow path member includes at least one discharge path extending toward the plurality of blades to spray washing water, with a nozzle formed at a front lower end thereof, and wherein, in a state where the rotation axis is inserted into the rotating boss, a distance (H2) between an upper surface of the rotating boss and a lower end of the rotation axis is greater than a distance (H1) between an upper end of the blade and a front lower end of the discharge path.

A laundry machine according to an embodiment of the present invention, comprises: a cabinet; a tub rotatably disposed within the cabinet; and a detergent supply device provided in the cabinet for supplying detergent to the tub, wherein the detergent supply device comprises: a housing fixedly mounted to the cabinet and connected to a water supply device; a drawer withdrawably mounted to the housing, and including a receiving portion recessed to accommodate detergent and a rotating boss extending upward from a bottom of the receiving portion; a mixing member rotatably disposed in the receiving portion to mix the detergent inside the receiving portion; and a flow path member that is connected to the housing at a position above the drawer and forms a flow path for supplying water from the water supply device to the drawer, wherein the mixing member includes: a rotation axis, and a plurality of blades extending radially from an outer surface of the rotation axis, wherein the flow path member includes at least one discharge path extending toward the plurality of blades to spray washing water, with a nozzle formed at a front lower end thereof, and wherein, in a state where the rotation axis is inserted into the rotating boss, a distance (H2) between an upper surface of the rotating boss and a lower end of the rotation axis is greater than a distance (H1) between an upper end of the blade and a front lower end of the discharge path.

Methods for mixing fluids including etanercept include providing a tank having an impeller with a 10 inch blade diameter disposed therein, filling a tank with a liquid composition comprising etanercept and one or more buffers to a level within the tank to cover the impeller, and operating the impeller to mix the liquid composition to a homogeneous state while maintaining a maximum shear stress imparted on the mixture of fluids at 125,000 s.sup.1 or below.

Methods for mixing fluids including etanercept include providing a tank having an impeller with a 10 inch blade diameter disposed therein, filling a tank with a liquid composition comprising etanercept and one or more buffers to a level within the tank to cover the impeller, and operating the impeller to mix the liquid composition to a homogeneous state while maintaining a maximum shear stress imparted on the mixture of fluids at 125,000 s.sup.1 or below.